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PRMT3: New Binding Molecule to RhoGDI-α During Mycophenolic Acid-induced β-cell Death

DC FieldValueLanguage
dc.contributor.author김명수-
dc.contributor.author김범석-
dc.contributor.author김유선-
dc.contributor.author조유리-
dc.contributor.author주동진-
dc.contributor.author허규하-
dc.date.accessioned2015-01-06T16:45:22Z-
dc.date.available2015-01-06T16:45:22Z-
dc.date.issued2014-
dc.identifier.issn0041-1345-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/98706-
dc.description.abstractMycophenolic acid (MPA)-induced beta cell toxicity limits islet graft survival. However, the signal transduction mechanisms underlying MPA-induced β-cell toxicity have not been fully elucidated. Previously, we showed that MPA-induced pancreatic β-cell apoptosis proceeds via RhoGDI-α down-regulation linked to Rac1 activation. In the present study, we investigated factors affecting RhoGDI-α during MPA-induced β-cell apoptosis. The presence of RhoGDI-α-related protein was determined with the use of yeast 2-hybrid (Y2H) analysis. Y2H screening of RhoGDI-α was performed in yeast PBN204 strain containing 3 reporters (URA3, lacZ, and ADE2) under the control of different GAL promoters. INS-1E cells (an insulin-secreting pancreatic β-cell line) were treated with MPA for 12, 24, and 36 hours. Eighty-three real positives were obtained by Y2H analysis, and of these, arginine N-methyltransferase 3 (PRMT3) protein interacted with RhoGDI-α in INS-1E cells. PRMT3 gene expressions and its protein levels were significantly decreased during MPA-induced apoptosis. In summary, PRMT3 and RhoGDI-α were found to interact in INS-1E cells. Furthermore, MPA was found to regulate this interaction in INS-1E cells by down-regulating the gene expression of PRMT3. These findings suggest that control of the interaction between PRMT3 and RhoGDI-α could be used to prevent MPA-induced β-cell death.-
dc.description.statementOfResponsibilityopen-
dc.format.extent1229~1232-
dc.relation.isPartOfTRANSPLANTATION PROCEEDINGS-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAnimals-
dc.subject.MESHApoptosis/drug effects*-
dc.subject.MESHCell Line, Tumor-
dc.subject.MESHGene Expression Regulation-
dc.subject.MESHImmunosuppressive Agents/toxicity*-
dc.subject.MESHInsulin-Secreting Cells/drug effects*-
dc.subject.MESHInsulin-Secreting Cells/enzymology-
dc.subject.MESHInsulin-Secreting Cells/pathology-
dc.subject.MESHMycophenolic Acid/toxicity*-
dc.subject.MESHProtein Binding-
dc.subject.MESHProtein-Arginine N-Methyltransferases/genetics-
dc.subject.MESHProtein-Arginine N-Methyltransferases/metabolism*-
dc.subject.MESHRats-
dc.subject.MESHTime Factors-
dc.subject.MESHTwo-Hybrid System Techniques-
dc.subject.MESHrho Guanine Nucleotide Dissociation Inhibitor alpha/genetics-
dc.subject.MESHrho Guanine Nucleotide Dissociation Inhibitor alpha/metabolism*-
dc.titlePRMT3: New Binding Molecule to RhoGDI-α During Mycophenolic Acid-induced β-cell Death-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Surgery (외과학)-
dc.contributor.googleauthorK.H. Huh-
dc.contributor.googleauthorY. Cho-
dc.contributor.googleauthorB.S. Kim-
dc.contributor.googleauthorD.J. Joo-
dc.contributor.googleauthorM.S. Kim-
dc.contributor.googleauthorY.S. Kim-
dc.identifier.doi10.1016/j.transproceed.2013.12.016-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA00488-
dc.contributor.localIdA00785-
dc.contributor.localIdA03872-
dc.contributor.localIdA03948-
dc.contributor.localIdA04344-
dc.contributor.localIdA00424-
dc.relation.journalcodeJ02755-
dc.identifier.eissn1873-2623-
dc.identifier.pmid24815167-
dc.identifier.urlhttp://www.sciencedirect.com/science/article/pii/S0041134513014127-
dc.contributor.alternativeNameKim, Myoung Soo-
dc.contributor.alternativeNameKim, Beom Seok-
dc.contributor.alternativeNameKim, Yu Seun-
dc.contributor.alternativeNameCho, Yu Ri-
dc.contributor.alternativeNameJoo, Dong Jin-
dc.contributor.alternativeNameHuh, Kyu Ha-
dc.contributor.affiliatedAuthorKim, Beom Seok-
dc.contributor.affiliatedAuthorKim, Yu Seun-
dc.contributor.affiliatedAuthorCho, Yu Ri-
dc.contributor.affiliatedAuthorJoo, Dong Jin-
dc.contributor.affiliatedAuthorHuh, Kyu Ha-
dc.contributor.affiliatedAuthorKim, Myoung Soo-
dc.rights.accessRightsfree-
dc.citation.volume46-
dc.citation.number4-
dc.citation.startPage1229-
dc.citation.endPage1232-
dc.identifier.bibliographicCitationTRANSPLANTATION PROCEEDINGS, Vol.46(4) : 1229-1232, 2014-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Surgery (외과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers

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