Cited 4 times in
PRMT3: New Binding Molecule to RhoGDI-α During Mycophenolic Acid-induced β-cell Death
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 김명수 | - |
dc.contributor.author | 김범석 | - |
dc.contributor.author | 김유선 | - |
dc.contributor.author | 조유리 | - |
dc.contributor.author | 주동진 | - |
dc.contributor.author | 허규하 | - |
dc.date.accessioned | 2015-01-06T16:45:22Z | - |
dc.date.available | 2015-01-06T16:45:22Z | - |
dc.date.issued | 2014 | - |
dc.identifier.issn | 0041-1345 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/98706 | - |
dc.description.abstract | Mycophenolic acid (MPA)-induced beta cell toxicity limits islet graft survival. However, the signal transduction mechanisms underlying MPA-induced β-cell toxicity have not been fully elucidated. Previously, we showed that MPA-induced pancreatic β-cell apoptosis proceeds via RhoGDI-α down-regulation linked to Rac1 activation. In the present study, we investigated factors affecting RhoGDI-α during MPA-induced β-cell apoptosis. The presence of RhoGDI-α-related protein was determined with the use of yeast 2-hybrid (Y2H) analysis. Y2H screening of RhoGDI-α was performed in yeast PBN204 strain containing 3 reporters (URA3, lacZ, and ADE2) under the control of different GAL promoters. INS-1E cells (an insulin-secreting pancreatic β-cell line) were treated with MPA for 12, 24, and 36 hours. Eighty-three real positives were obtained by Y2H analysis, and of these, arginine N-methyltransferase 3 (PRMT3) protein interacted with RhoGDI-α in INS-1E cells. PRMT3 gene expressions and its protein levels were significantly decreased during MPA-induced apoptosis. In summary, PRMT3 and RhoGDI-α were found to interact in INS-1E cells. Furthermore, MPA was found to regulate this interaction in INS-1E cells by down-regulating the gene expression of PRMT3. These findings suggest that control of the interaction between PRMT3 and RhoGDI-α could be used to prevent MPA-induced β-cell death. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 1229~1232 | - |
dc.relation.isPartOf | TRANSPLANTATION PROCEEDINGS | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Apoptosis/drug effects* | - |
dc.subject.MESH | Cell Line, Tumor | - |
dc.subject.MESH | Gene Expression Regulation | - |
dc.subject.MESH | Immunosuppressive Agents/toxicity* | - |
dc.subject.MESH | Insulin-Secreting Cells/drug effects* | - |
dc.subject.MESH | Insulin-Secreting Cells/enzymology | - |
dc.subject.MESH | Insulin-Secreting Cells/pathology | - |
dc.subject.MESH | Mycophenolic Acid/toxicity* | - |
dc.subject.MESH | Protein Binding | - |
dc.subject.MESH | Protein-Arginine N-Methyltransferases/genetics | - |
dc.subject.MESH | Protein-Arginine N-Methyltransferases/metabolism* | - |
dc.subject.MESH | Rats | - |
dc.subject.MESH | Time Factors | - |
dc.subject.MESH | Two-Hybrid System Techniques | - |
dc.subject.MESH | rho Guanine Nucleotide Dissociation Inhibitor alpha/genetics | - |
dc.subject.MESH | rho Guanine Nucleotide Dissociation Inhibitor alpha/metabolism* | - |
dc.title | PRMT3: New Binding Molecule to RhoGDI-α During Mycophenolic Acid-induced β-cell Death | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Surgery (외과학) | - |
dc.contributor.googleauthor | K.H. Huh | - |
dc.contributor.googleauthor | Y. Cho | - |
dc.contributor.googleauthor | B.S. Kim | - |
dc.contributor.googleauthor | D.J. Joo | - |
dc.contributor.googleauthor | M.S. Kim | - |
dc.contributor.googleauthor | Y.S. Kim | - |
dc.identifier.doi | 10.1016/j.transproceed.2013.12.016 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A00488 | - |
dc.contributor.localId | A00785 | - |
dc.contributor.localId | A03872 | - |
dc.contributor.localId | A03948 | - |
dc.contributor.localId | A04344 | - |
dc.contributor.localId | A00424 | - |
dc.relation.journalcode | J02755 | - |
dc.identifier.eissn | 1873-2623 | - |
dc.identifier.pmid | 24815167 | - |
dc.identifier.url | http://www.sciencedirect.com/science/article/pii/S0041134513014127 | - |
dc.contributor.alternativeName | Kim, Myoung Soo | - |
dc.contributor.alternativeName | Kim, Beom Seok | - |
dc.contributor.alternativeName | Kim, Yu Seun | - |
dc.contributor.alternativeName | Cho, Yu Ri | - |
dc.contributor.alternativeName | Joo, Dong Jin | - |
dc.contributor.alternativeName | Huh, Kyu Ha | - |
dc.contributor.affiliatedAuthor | Kim, Beom Seok | - |
dc.contributor.affiliatedAuthor | Kim, Yu Seun | - |
dc.contributor.affiliatedAuthor | Cho, Yu Ri | - |
dc.contributor.affiliatedAuthor | Joo, Dong Jin | - |
dc.contributor.affiliatedAuthor | Huh, Kyu Ha | - |
dc.contributor.affiliatedAuthor | Kim, Myoung Soo | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 46 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 1229 | - |
dc.citation.endPage | 1232 | - |
dc.identifier.bibliographicCitation | TRANSPLANTATION PROCEEDINGS, Vol.46(4) : 1229-1232, 2014 | - |
dc.identifier.rimsid | 38552 | - |
dc.type.rims | ART | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.