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Broth Microdilution Methods Using Β-Lactamase Inhibitors for the Identification of Klebsiella pneumoniae Carbapenemases and Metallo-Β-Lactamases in Gram-Negative Bacilli

Authors
 Seok Hoon Jeong  ;  Wonkeun Song  ;  Il Kwon Bae  ;  Han-Sung Kim  ;  Jae-Seok Kim  ;  Min-Jeong Park  ;  Dong Hoon Shin 
Citation
 ANNALS OF CLINICAL AND LABORATORY SCIENCE, Vol.44(1) : 49-55, 2014 
Journal Title
 ANNALS OF CLINICAL AND LABORATORY SCIENCE 
ISSN
 0091-7370 
Issue Date
2014
MeSH
Bacillus/drug effects ; Bacillus/enzymology* ; Bacterial Proteins/metabolism* ; Humans ; Klebsiella pneumoniae/drug effects ; Klebsiella pneumoniae/enzymology* ; Microbial Sensitivity Tests/methods* ; Sensitivity and Specificity ; beta-Lactamase Inhibitors/pharmacology* ; beta-Lactamases/metabolism*
Abstract
This study was designed to evaluate the performance of the broth microdilution (BMD) test in detecting the production of Klebsiella pneumoniae carbapenemases (KPCs) and metallo-β-lactamases (MBLs) in clinical isolates of gram-negative bacilli by using various β-lactamase inhibitors. A carbapenemase detection test, comprised of Mueller-Hinton broth containing serial twofold dilutions of imipenem or meropenem with and without aminophenylboronic acid (APB), phenylboronic acid (PB), cloxacillin (CLX), dipicolinic acid (DPA), or EDTA, was evaluated against 31 Klebsiella pneumoniae strains with KPC, 21 MBL-producers (3 Enterobacteriaceae and 18 non-fermenters), and 26 carbapenemase-non-producing Enterobacteriaceae strains. A threefold or greater decrease in the MIC of imipenem or meropenem with β-lactamase inhibitors, when compared with the imipenem or meropenem alone, was considered a positive result. Imipenem with and without PB had the most comparable sensitivity (93.5%) and specificity (93.6%) for the detection of K. pneumoniae with KPC enzymes if the additional criterion of a negative CLX result was included. Both DPA and EDTA had excellent sensitivity (imipenem with and without DPA vs. EDTA, 90.5% vs. 95.2%) and specificity (imipenem with and without DPA vs. EDTA, 98.2% vs. 100%) for the detection of MBL-producing gram-negative bacilli. The comparative study showed that, by using imipenem with and without PB, CLX, and EDTA, the BMD test was effective in detecting KPC and MBL enzymes. The BMD test could be applied for routine use in commercially available semiautomated systems for the detection of KPCs and MBLs in gram-negative bacilli.
Full Text
http://www.annclinlabsci.org/content/44/1/49.long
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
Yonsei Authors
Bae, Il Kwon(배일권)
Jeong, Seok Hoon(정석훈) ORCID logo https://orcid.org/0000-0001-9290-897X
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/98394
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