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Rapid upregulation and clearance of distinct circulating microRNAs after prolonged aerobic exercise

Authors
 Aaron L. Baggish  ;  Joseph Park  ;  Pil-Ki Min  ;  Stephanie Isaacs  ;  Beth A. Parker  ;  Paul D. Thompson  ;  Chris Troyanos  ;  Pierre D'Hemecourt  ;  Sophia Dyer  ;  Marissa Thiel  ;  Andrew Hale  ;  Stephen Y. Chan 
Citation
 JOURNAL OF APPLIED PHYSIOLOGY , Vol.116(5) : 522-531, 2014 
Journal Title
 JOURNAL OF APPLIED PHYSIOLOGY 
ISSN
 0021-8987 
Issue Date
2014
MeSH
Adult ; Biomarkers ; C-Reactive Protein/metabolism ; Creatine Kinase/blood ; Exercise/physiology* ; Humans ; Male ; MicroRNAs/metabolism* ; Middle Aged ; Muscle, Skeletal/metabolism ; Myocardium/pathology ; Natriuretic Peptide, Brain/metabolism ; Peptide Fragments/metabolism ; Physical Endurance/physiology ; Real-Time Polymerase Chain Reaction ; Rest/physiology ; Running/physiology ; Stress, Physiological ; Troponin/metabolism ; Up-Regulation/physiology ; Young Adult
Keywords
cardiorespiratory fitness biomarker ; cardiovascular biomarker ; circulating microRNA ; exercise physiology ; prolonged aerobic exercise
Abstract
Short nonprotein coding RNA molecules, known as microRNAs (miRNAs), are intracellular mediators of adaptive processes, including muscle hypertrophy, contractile force generation, and inflammation. During basal conditions and tissue injury, miRNAs are released into the bloodstream as “circulating” miRNAs (c-miRNAs). To date, the impact of extended-duration, submaximal aerobic exercise on plasma concentrations of c-miRNAs remains incompletely characterized. We hypothesized that specific c-miRNAs are differentially upregulated following prolonged aerobic exercise. To test this hypothesis, we measured concentrations of c-miRNAs enriched in muscle (miR-1, miR-133a, miR-499–5p), cardiac tissue (miR-208a), and the vascular endothelium (miR-126), as well as those important in inflammation (miR-146a) in healthy male marathon runners (N = 21) at rest, immediately after a marathon (42-km foot race), and 24 h after the race. In addition, we compared c-miRNA profiles to those of conventional protein biomarkers reflective of skeletal muscle damage, cardiac stress and necrosis, and systemic inflammation. Candidate c-miRNAs increased immediately after the marathon and declined to prerace levels or lower after 24 h of race completion. However, the magnitude of change for each c-miRNA differed, even when originating from the same tissue type. In contrast, traditional biomarkers increased after exercise but remained elevated 24 h postexercise. Thus c-miRNAs respond differentially to prolonged exercise, suggesting the existence of specific mechanisms of c-miRNA release and clearance not fully explained by generalized cellular injury. Furthermore, c-miRNA expression patterns differ in a temporal fashion from corollary conventional tissue-specific biomarkers, emphasizing the potential of c-miRNAs as unique, real-time markers of exercise-induced tissue adaptation.
Full Text
http://jap.physiology.org/content/116/5/522
DOI
10.1152/japplphysiol.01141.2013
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
Yonsei Authors
Min, Pil Ki(민필기) ORCID logo https://orcid.org/0000-0001-7033-7651
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/98287
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