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Enhanced Neural Cell Adhesion and Neurite Outgrowth on Graphene-Based Biomimetic Substrates

DC FieldValueLanguage
dc.contributor.author박종철-
dc.contributor.author이미희-
dc.date.accessioned2015-01-06T16:25:07Z-
dc.date.available2015-01-06T16:25:07Z-
dc.date.issued2014-
dc.identifier.issn2314-6133-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/98068-
dc.description.abstractNeural cell adhesion and neurite outgrowth were examined on graphene-based biomimetic substrates. The biocompatibility of carbon nanomaterials such as graphene and carbon nanotubes (CNTs), that is, single-walled and multiwalled CNTs, against pheochromocytoma-derived PC-12 neural cells was also evaluated by quantifying metabolic activity (with WST-8 assay), intracellular oxidative stress (with ROS assay), and membrane integrity (with LDH assay). Graphene films were grown by using chemical vapor deposition and were then coated onto glass coverslips by using the scooping method. Graphene sheets were patterned on SiO2/Si substrates by using photolithography and were then covered with serum for a neural cell culture. Both types of CNTs induced significant dose-dependent decreases in the viability of PC-12 cells, whereas graphene exerted adverse effects on the neural cells just at over 62.5 ppm. This result implies that graphene and CNTs, even though they were the same carbon-based nanomaterials, show differential influences on neural cells. Furthermore, graphene-coated or graphene-patterned substrates were shown to substantially enhance the adhesion and neurite outgrowth of PC-12 cells. These results suggest that graphene-based substrates as biomimetic cues have good biocompatibility as well as a unique surface property that can enhance the neural cells, which would open up enormous opportunities in neural regeneration and nanomedicine.-
dc.description.statementOfResponsibilityopen-
dc.formatapplication/pdf-
dc.relation.isPartOfBioMed Research International-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.titleEnhanced Neural Cell Adhesion and Neurite Outgrowth on Graphene-Based Biomimetic Substrates-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Medical Engineering (의학공학)-
dc.contributor.googleauthorSuck Won Hong-
dc.contributor.googleauthorJong Ho Lee-
dc.contributor.googleauthorSeok Hee Kang-
dc.contributor.googleauthorEun Young Hwang-
dc.contributor.googleauthorYu-Shik Hwang-
dc.contributor.googleauthorMi Hee Lee-
dc.contributor.googleauthorDong-Wook Han-
dc.contributor.googleauthorJong-Chul Park-
dc.identifier.doi10.1155/2014/212149-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA01662-
dc.contributor.localIdA02777-
dc.relation.journalcodeJ00315-
dc.contributor.alternativeNamePark, Jong Chul-
dc.contributor.alternativeNameLee, Mi Hee-
dc.contributor.affiliatedAuthorPark, Jong Chul-
dc.contributor.affiliatedAuthorLee, Mi Hee-
dc.citation.volume2014-
dc.citation.startPage212149-
dc.identifier.bibliographicCitationBioMed Research International, Vol.2014 : 212149, 2014-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Medical Engineering (의학공학교실) > 1. Journal Papers

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