Sinus Augmentation Using BMP-2 in a Bovine Hydroxyapatite/Collagen Carrier in Dogs

Abstract

AIM: The objective of this study was to determine the efficacy of bone morphogenetic protein 2 (BMP-2) in a bovine hydroxyapatite/collagen (BHC) carrier to augment bone formation in a canine nasal sinus model. METHODS: Eight mongrel dogs, approximately 12 months old and 30 kg in weight were used. Following preparation of bilateral sinus access windows, BHC alone (control) or loaded with E. coli-derived BMP-2 at 0.1 mg/ml was implanted in four animals, and BHC loaded with E. coli-derived BMP-2 at 0.5 and 1.5 mg/ml was implanted in four animals. The animals were euthanized at 20 weeks when block sections were obtained for micro-computed tomography and histometric analyses. RESULTS: Total augmented volumes did not differ significantly between groups. Histometric analysis showed significantly enhanced bone formation for the BMP-2 groups compared with control. CONCLUSION: BMP-2 in a BHC carrier, even at the low 0.1-mg/ml concentration, induces osteogenic activity, enhancing local bone formation in a canine sinus model.