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Diagnosis of diabetes mellitus using sialic acid expression of erythrocyte and a microfluidic resistive temperature detector (micro-RTD).

Authors
 Chenjie Yi  ;  Jung-Hyun Lee  ;  Bong Seop Kwak  ;  Ming Xian Lin  ;  Hyun Ok Kim  ;  Hyo-Il Jung 
Citation
 Sensors and Actuators B-Chemical, Vol.191(1) : 305-312, 2014 
Journal Title
 Sensors and Actuators B-Chemical 
ISSN
 0925-4005 
Issue Date
2014
Abstract
Sialic acid (SA, N-acetylneuraminic acid), a negatively charged monosaccharide, is expressed less frequently in diabetes patients than in normal people. A simple method for differentiating abnormal erythrocytes from normal erythrocytes based on SA-expression can be a useful diagnostic tool for diabetes. This study developed such a system by first modifying a sensor surface with SA recognition molecules to capture SA-expressing erythrocytes. Second, the sensor surface was thoroughly washed to remove abnormal erythrocytes while the normal erythrocytes remained. The remaining cells from the patient's and normal samples were heated with a 532 nm wavelength laser and temperature changes were measured using a resistive temperature detector (RTD). Hemoglobin molecules in the erythrocytes absorb a specific wavelength of photons (532 nm), which is reflected as a temperature change. In total, 17 samples from diabetes patients and 9 samples from normal patients were applied to the study system. The average temperature difference (0.32 °C) between diabetics and non-diabetics was significant, indicating that the study system can be utilized for diagnosis of diabetes mellitus. Moreover, a microfluidic channel using PDMS (poly-dimethylsiloxane) was fabricated and integrated onto the RTD to precisely deliver the cells. The process described above was repeated and almost identical results were obtained, suggesting that the microfluidic RTD has promising clinical applications.
Full Text
http://www.sciencedirect.com/science/article/pii/S0925400513011878
DOI
10.1016/j.snb.2013.10.004
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
Yonsei Authors
김현옥(Kim, Hyun Ok) ORCID logo https://orcid.org/0000-0002-4964-1963
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URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/97930
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