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In vitro Expansion of Mesenchymal Stem Cells using 3D Matrix Derived from Cardiac Fibroblast

Authors
 Woo Chul Chang  ;  So Yeon Lim  ;  Ki Chul Hwang  ;  Nam Sik Chung  ;  Yang Soo Jang  ;  Byung Wook Song  ;  Sun Ju Lee  ;  Hee Sang Song 
Citation
 TISSUE ENGINEERING AND REGENERATIVE MEDICINE, Vol.4(3) : 370-375, 2007 
Journal Title
 TISSUE ENGINEERING AND REGENERATIVE MEDICINE 
ISSN
 1738-2696 
Issue Date
2007
Abstract
Mesenchymal stem cells (MSC), nonhematopoietic progenitor cells are capable of differentiating into multiple lineage of the mesenchyme such as bone, cartilage, tendon, fat, heart, muscle, and brain, in vitro and in vivo. Although autologous MSC have some advantageous factors, MSC have very poor replicative capacity. Previous studies indicated that growth factors but also cell seeding density made an effect on expansion of MSCs. In this study, we examined the growth rate and adhesion of MSC in tissue culture coated with a basement membrane-like extracellular matrix. When self-renewal of MSC were compared between 2-D and 3-D, the growth rate of MSCs on 3-D matrix was highest among plastic, Fibronectin- and 3-D matrix plate but also attachment of MSCs on 3-D matrix was 6-fold greater than that on plastic. MSCs plated into 3-D matrix showed colocalization of α5 integrin and αV integrin. This study may reflect that the extracellular matrix had greater effect on the expansion of MSCs because this distinctive in 3-D matrix adhesion differ in localization and function from classically described in vitro adhesion.
Files in This Item:
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Appears in Collections:
1. College of Medicine (의과대학) > BioMedical Science Institute (의생명과학부) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
Yonsei Authors
Lee, Sun Ju(이선주)
Jang, Yang Soo(장양수) ORCID logo https://orcid.org/0000-0002-2169-3112
Chung, Nam Sik(정남식)
Hwang, Ki Chul(황기철)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/97404
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