Cited 29 times in
Molecular cloning and the allergenic characterization of tropomyosin from Tyrophagus putrescentiae
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 용태순 | - |
dc.contributor.author | 이인용 | - |
dc.contributor.author | 이종원 | - |
dc.contributor.author | 이한일 | - |
dc.contributor.author | 이해석 | - |
dc.contributor.author | 정경용 | - |
dc.contributor.author | 홍천수 | - |
dc.date.accessioned | 2014-12-21T17:17:55Z | - |
dc.date.available | 2014-12-21T17:17:55Z | - |
dc.date.issued | 2007 | - |
dc.identifier.issn | 0929-8665 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/97325 | - |
dc.description.abstract | Storage mites have been recognized as a cause of asthma and rhinitis. Studies from several countries have shown that the IgE-mediated allergy to storage mites is of considerable importance, especially in rural populations. This study aimed to identify and characterize new allergens from Tyrophagus putrescentiae. A partial cDNA sequence encoding tropomyosin was isolated from the cDNA library by immunoscreening using anti-mouse IgG1 sera raised against T. putrescentiae whole body extract. The deduced amino acid sequence shares 64-94% identity with previously known allergenic tropomyosins. Its recombinant protein was produced by using a pET 28b expression system and purified by affinity chromatography using Ni-NTA agarose. The IgE reactivities of tropomyosins from T. putrescentiae and Dermatophagoides farinae were compared by enzyme linked immunosorbent assay (ELISA). Recombinant Tyr p 10 showed 12.5% (5/40) IgE-binding reactivity, whereas recombinant Der f 10 showed 25% (10/40) IgE-binding reactivity against the same sera from storage mite-sensitized and house dust mite-sensitized subjects. Both recombinant Tyr p 10 and Der f 10 showed little inhibition of IgE binding to T. putrescentiae crude extract by ELISA. Tropomyosin seems to contribute only a small portion of the cross-reactivity with house dust mites. | - |
dc.description.statementOfResponsibility | open | - |
dc.relation.isPartOf | PROTEIN AND PEPTIDE LETTERS | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | Molecular cloning and the allergenic characterization of tropomyosin from Tyrophagus putrescentiae | - |
dc.type | Article | - |
dc.contributor.college | Researcher Institutes (부설 연구소) | - |
dc.contributor.department | Institute of Allergy (알레르기연구소) | - |
dc.contributor.googleauthor | Kyoung Yong Jeong | - |
dc.contributor.googleauthor | Haeseok Lee | - |
dc.contributor.googleauthor | Tai-Soon Yong | - |
dc.contributor.googleauthor | Chein-Soo Hong | - |
dc.contributor.googleauthor | Han-Il Ree | - |
dc.contributor.googleauthor | In-Yong Lee | - |
dc.contributor.googleauthor | Jongweon Lee | - |
dc.contributor.googleauthor | Jae Sik Lee | - |
dc.identifier.doi | 10.2174/092986607780782777 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A02424 | - |
dc.contributor.localId | A03059 | - |
dc.contributor.localId | A03077 | - |
dc.contributor.localId | A03144 | - |
dc.contributor.localId | A03279 | - |
dc.contributor.localId | A03281 | - |
dc.contributor.localId | A03572 | - |
dc.contributor.localId | A04448-1 | - |
dc.relation.journalcode | J02562 | - |
dc.identifier.eissn | 1875-5305 | - |
dc.identifier.url | http://www.eurekaselect.com/78274/article | - |
dc.contributor.alternativeName | Yong, Tai Soon | - |
dc.contributor.alternativeName | Lee, In Yong | - |
dc.contributor.alternativeName | Lee, Jae Sik | - |
dc.contributor.alternativeName | Lee, Jong Weon | - |
dc.contributor.alternativeName | Ree, Han Il | - |
dc.contributor.alternativeName | Lee, Hae Seok | - |
dc.contributor.alternativeName | Jeong, Kyoung Yong | - |
dc.contributor.alternativeName | Hong, Chein Soo | - |
dc.contributor.affiliatedAuthor | Yong, Tai Soon | - |
dc.contributor.affiliatedAuthor | Lee, In Yong | - |
dc.contributor.affiliatedAuthor | Lee, Jong Weon | - |
dc.contributor.affiliatedAuthor | Ree, Han Il | - |
dc.contributor.affiliatedAuthor | Lee, Hae Seok | - |
dc.contributor.affiliatedAuthor | Jeong, Kyoung Yong | - |
dc.contributor.affiliatedAuthor | Hong, Chein Soo | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 14 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 431 | - |
dc.citation.endPage | 436 | - |
dc.identifier.bibliographicCitation | PROTEIN AND PEPTIDE LETTERS, Vol.14(5) : 431-436, 2007 | - |
dc.identifier.rimsid | 50440 | - |
dc.type.rims | ART | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.