Cited 48 times in
Genetic variation in the cysteine biosynthesis pathway causes sensitivity to pharmacological compounds
DC Field | Value | Language |
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dc.contributor.author | 김현석 | - |
dc.date.accessioned | 2014-12-21T17:17:34Z | - |
dc.date.available | 2014-12-21T17:17:34Z | - |
dc.date.issued | 2007 | - |
dc.identifier.issn | 0027-8424 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/97314 | - |
dc.description.abstract | Complex traits are the product of multiple genes with effects that depend on both the genetic and environmental background. Although this complexity makes a comprehensive genetic analysis difficult, identification of even a single gene provides insight into the biochemical and/or signaling pathway underlying a trait. However, it is unknown whether multiple pathways, and consequently multiple genes, must be identified to adequately understand a trait's molecular basis. Using crosses between three natural isolates of Saccharomyces cerevisiae, we mapped sensitivity to a number of pharmacologically active compounds to a single nonsynonymous polymorphism in cystathione-β-synthase (CYS4), which is required for the first committed step in the cysteine biosynthesis pathway. Drug sensitivity is mediated by a deficiency in cysteine and consequently glutathione production, because drug sensitivity is abrogated by cysteine or glutathione supplementation. Within a diverse panel of 60 natural yeast isolates, the drug-sensitive CYS4 allele is rare, and glutathione supplementation failed to alleviate drug-dependent growth defects in two other drug-sensitive strains. These results implicate the cysteine/glutathione biosynthesis pathway as a significant, but not the sole contributor to pharmacological variation in yeast | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 19387~19391 | - |
dc.relation.isPartOf | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | Genetic variation in the cysteine biosynthesis pathway causes sensitivity to pharmacological compounds | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Medical Research Center (임상의학연구센터) | - |
dc.contributor.googleauthor | Hyun Seok Kim | - |
dc.contributor.googleauthor | Justin C. Fay | - |
dc.identifier.doi | 10.1073/pnas.0708194104 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A01112 | - |
dc.relation.journalcode | J02550 | - |
dc.identifier.eissn | 1091-6490 | - |
dc.identifier.url | http://www.pnas.org/content/104/49/19387.short | - |
dc.contributor.alternativeName | Kim, Hyon Suk | - |
dc.contributor.affiliatedAuthor | Kim, Hyon Suk | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 104 | - |
dc.citation.number | 49 | - |
dc.citation.startPage | 19387 | - |
dc.citation.endPage | 19391 | - |
dc.identifier.bibliographicCitation | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, Vol.104(49) : 19387-19391, 2007 | - |
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