MPSS profiling of embryonic gonad and primordial germ cells in chicken
Authors
Heebal Kim ; Tae Sub Park ; Jae Yong Han ; Jeong Mook Lim ; Yeonkyung Kang ; Jungrye Nam ; Tae Min Kim ; Sang Su Shin ; Mi A Kim ; Kyung Je Park ; Sang Hyun Park ; Seon Duk Lee ; Jin Gyoung Jung ; Ji Hye Shin ; Jin Nam Kim ; Sunjin Moon ; Woon Kyu Lee
The massively parallel signature sequencing (MPSS) provides a greater depth of coverage than expressed sequence tag scan or microarray and provides a comprehensive expression profile. We used the MPSS technology to uncover gene expression profiling in the early embryonic gonads and primordial germ cells (PGCs) in the chicken. Total numbers of sequenced signatures were 1,012,533 and 995,676 for the PGCs and gonad, respectively. Using a noise distribution model, we found that 1.67% of all signatures are expressed at a higher level in PCGs and 2.81% of all signatures are expressed at a higher level in the gonad. The MPSS data are presented via an interactive web interface available at http://snugenome.snu.ac.kr/MPSS. The MPSS data have been submitted to the Gene Expression Omnibus of the National Center for Biotechnology Information (accession number GSM137300 and GSM137301 for PGCs and gonad, respectively).