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Molecular cloning and characterization of a cytosolic heat shock protein 70 from Naegleria fowleri

Authors
 Kyoung-Ju Song  ;  Kyung-Hui Song  ;  Ho-Joon Shin  ;  Kyung-Il Im  ;  Jhang-Ho Pak  ;  Sun Park  ;  Daeho Kwon  ;  Jong-Hyun Kim  ;  Byoung-Kuk Na 
Citation
 PARASITOLOGY RESEARCH, Vol.100(5) : 1083-1089, 2007 
Journal Title
PARASITOLOGY RESEARCH
ISSN
 0932-0113 
Issue Date
2007
Abstract
A gene encoding a cytosolic heat shock protein 70 from pathogenic Naegleria fowleri (Nf-cHSP70) was identified. The Nf-cHSP70 was 2,062 bp in length with an open reading frame of 1,980 bp encoding 659 amino acid residues. The deduced amino acid sequence of the gene shared high sequence identities with HSP70s from other parasitic organisms and mammals. The characteristic domains, including N-terminal ATPase domain, calmodulin-binding domain, and EE(D)VD motif, found in HSP70s were also well conserved in this gene. The recombinant Nf-cHSP70 protein showed strong antigenicity against the sera from mice experimentally infected with N. fowleri. Immunofluorescence assay showed that Nf-cHSP70 localized in cytosol of the parasite. The results from semi-quantitative RT-PCR and Western blot analyses demonstrated the expression levels of gene transcripts, and its products were significantly increased at high temperature (42 degrees C). The definitive biological roles of Nf-cHSP70 are not clear, but it may protect the parasite under environmental changes especially high temperature
Full Text
http://link.springer.com/article/10.1007%2Fs00436-006-0404-8
DOI
10.1007/s00436-006-0404-8
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Environmental Medical Biology (환경의생물학교실) > 1. Journal Papers
Yonsei Authors
Im, Kyung Il(임경일)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/97266
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