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Molecular cloning and characterization of a major egg antigen in Paragonimus westermani and its use in ELISA for the immunodiagnosis of paragonimiasis

Authors
 Jae Sik Lee  ;  Jongweon Lee  ;  Tai-Soon Yong  ;  Sun Hyun Kim 
Citation
 PARASITOLOGY RESEARCH, Vol.100(4) : 677-681, 2007 
Journal Title
PARASITOLOGY RESEARCH
ISSN
 0932-0113 
Issue Date
2007
Abstract
A recombinant protein of a Paragonimus westermani egg antigen was produced and tested as an antigen for the serologic diagnosis of P. westermani infection. The P. westermani egg antigen gene contains a single open reading frame of 966 base pairs encoding 322 amino acids from 5′ methionine to the 3′ stop codon. The predicted amino acid sequence of this egg antigen was 40, 38, and was 35% identical to heat shock proteins from Schistosoma japonicum, Schistosoma mansoni, and Taenia saginata. The distribution this antigen was investigated in adult worms by indirect immunofluorescence assay, and found to be distributed in eggs and uteri. The specificity and sensitivity of the recombinant antigen were assessed by enzyme-linked immunosorbent assay (ELISA) using sera from patients infected with different parasites, which included 41 patients with paragonimiasis, and negative controls. The diagnostic positive and negative predictive absorbance value was 0.24 and the sensitivity of ELISA using the recombinant antigen was 90.2%, and its specificity 100%. Our results suggest that the developed recombinant major egg antigen-based ELISA offers a highly sensitive and specific assay for the diagnosis of paragonimiasis.
Full Text
http://link.springer.com/article/10.1007%2Fs00436-006-0324-7
DOI
10.1007/s00436-006-0324-7
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Environmental Medical Biology (환경의생물학교실) > 1. Journal Papers
Yonsei Authors
Yong, Tai Soon(용태순) ORCID logo https://orcid.org/0000-0002-3445-0769
Lee, Jae Sik(이재식)
Lee, Jong Weon(이종원)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/97265
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