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인간 BMP-2-생리기능 복합 바이오활성 세라믹스를 이용한 조골 세포 분화 유도

Other Titles
 Osteoinduction using by Human BMP-2-physio-functional Bioactive Ceramics 
Authors
 김향  ;  문성환  ;  이환모  ;  박민  ;  김현민  ;  이정욱 
Citation
 Journal of the Korean Orthopaedic Association (대한정형외과학회지), Vol.42(3) : 386-394, 2007 
Journal Title
 Journal of the Korean Orthopaedic Association (대한정형외과학회지) 
ISSN
 1226-2102 
Issue Date
2007
Keywords
Calcium phosphate-rhBMP-2 composite ; Surface coating technique ; Osteoinductivity
Abstract
Purpose: To assess the properties and the osteogenic potency of the calcium phosphate-recombinant human morphogenetic protein-2 (CaP-rhBMP-2 composite) on glass-ceramics. Materials and Methods: Bioactive glass-ceramics, as a scaffold, and a calcium phosphate (CaP) solution (pH7.4) were prepared. Recombinant human bone morphogenetic protein-2 (rhBMP-2) was purified from CHO-K1 cells by transfecting the cells with BMP-2 cDNA. The glass-ceramics were soaked for 3 days at room temperature in saline, a CaP only solution, and a CaP solution containing rhBMP-2. Scanning electron microscopy (SEM), Fourier transform infrared reflection spectroscopy (FT-IR), thin film X-ray diffraction (TF-XRD) and immunofluorescent staining (IF) of the anti-human BMP-2 tot composite-coated scaffold were performed to verify the characterization of the scaffold surface. In addition, RT-PCR of osteogenic marker gene and SEM photography were performed after adhering the mouse preosteoblast MC3T3-E1 cells in order to assess the osteoinductivity. Results: CaP-rhBMP-2 composite was coated on the surface of glass-ceramics, as confirmed by SEM, FT-IR, TF-XRD spectrum, and IF. The CaP-rhBMP-2 composite on the glass-ceramic showed a globular shape covered with fine spikes while the CaP on the glass-ceramic showed a fine spike structure on the flat glass surface. The expression of collagen type Ⅰ and alkaline phosphatase mRNAs had increased 4 hours after cell seeding. In addition, the level of osteocalcin mRNA ex-pression had increased significantly by 3 days in the CaP-rhBMP-2 composite compared with the control and CaP group. The SEM photographs showed more active filopodia formation in the CaP-rhBMP-2 composite than the other groups. There was extensive newly synthesized extracellular matrix around the osteoblasts and CaP-rhBMP-2 composite nodule. Conclusion: The application of Cap-rhBMP-2 composite-surface coating technique on bioactive glass-ceramic is a powerful tool for osteoinduction.
Files in This Item:
T200700906.pdf Download
DOI
10.4055/jkoa.2007.42.3.386
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Orthopedic Surgery (정형외과학교실) > 1. Journal Papers
Yonsei Authors
Moon, Seong Hwan(문성환)
Lee, Hwan Mo(이환모) ORCID logo https://orcid.org/0000-0002-5405-3832
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/96645
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