Cited 59 times in
(-)-Epigallocatechin gallate induces apoptosis, via caspase activation, in osteoclasts differentiated from RAW 264.7 cells
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 김종관 | - |
dc.contributor.author | 김창성 | - |
dc.contributor.author | 조규성 | - |
dc.contributor.author | 채중규 | - |
dc.contributor.author | 최성호 | - |
dc.date.accessioned | 2014-12-21T16:52:26Z | - |
dc.date.available | 2014-12-21T16:52:26Z | - |
dc.date.issued | 2007 | - |
dc.identifier.issn | 0022-3484 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/96513 | - |
dc.description.abstract | BACKGROUND AND OBJECTIVE: Alveolar bone resorption is a characteristic feature of periodontal diseases and involves removal of both the mineral and the organic constituents of the bone matrix, a process mainly carried out by multinucleated osteoclast cells. (-)-Epigallocatechin gallate, the main constituent of green tea polyphenols, has been reported to induce the apoptotic cell death of osteoclasts and to modulate caspase activation in various tumor cells. In the present study, we investigated the inhibitory effect of (-)-epigallocatechin gallate on osteoclast survival and examined if (-)-epigallocatechin gallate mediates osteoclast apoptosis via caspase activation. MATERIAL AND METHODS: The effect of (-)-epigallocatechin gallate on osteoclast survival was examined by tartrate-resistant acid phosphatase (TRAP) staining in osteoclasts differentiated from RAW 264.7 cells. In addition, we evaluated the apoptosis of osteoclasts by (-)-epigallocatechin gallate using a DNA-fragmentation assay. Involvement of caspase in (-)-epigallocatechin gallate-mediated osteoclast apoptosis was evaluated by treatment with a general caspase inhibitor, Z-VAD-FMK. Moreover, the effect of (-)-epigallocatechin gallate on the activation of caspase-3 was assessed by a colorimetric activity assay and western blotting. RESULTS: (-)-Epigallocatechin gallate significantly inhibited, in a dose-dependent manner, the survival of osteoclasts differentiated from RAW 264.7 cells and induced the apoptosis of osteoclasts. Treatment with (-)-epigallocatechin gallate resulted in DNA fragmentation and induced the activation of caspase-3 in RAW 264.7 cell-derived osteoclasts. Additional treatment with Z-VAD-FMK suppressed these effects of (-)-epigallocatechin gallate. CONCLUSION: From these findings, we could suggest that (-)-epigallocatechin gallate might prevent alveolar bone resorption by inhibiting osteoclast survival through the caspase-mediated apoptosis. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 212~218 | - |
dc.relation.isPartOf | JOURNAL OF PERIODONTAL RESEARCH | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Acid Phosphatase/analysis | - |
dc.subject.MESH | Alveolar Process/drug effects | - |
dc.subject.MESH | Amino Acid Chloromethyl Ketones/pharmacology | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Apoptosis/physiology* | - |
dc.subject.MESH | Bone Resorption/drug therapy* | - |
dc.subject.MESH | Caspase Inhibitors | - |
dc.subject.MESH | Caspases/drug effects | - |
dc.subject.MESH | Caspases/metabolism* | - |
dc.subject.MESH | Catechin/analogs & derivatives* | - |
dc.subject.MESH | Catechin/pharmacology | - |
dc.subject.MESH | Cell Line | - |
dc.subject.MESH | Cell Survival/drug effects | - |
dc.subject.MESH | DNA Fragmentation | - |
dc.subject.MESH | Enzyme Activation | - |
dc.subject.MESH | Enzyme Inhibitors/pharmacology | - |
dc.subject.MESH | Isoenzymes/analysis | - |
dc.subject.MESH | Mice | - |
dc.subject.MESH | Osteoclasts/drug effects* | - |
dc.subject.MESH | Osteoclasts/physiology | - |
dc.subject.MESH | RANK Ligand/metabolism | - |
dc.subject.MESH | Tartrate-Resistant Acid Phosphatase | - |
dc.title | (-)-Epigallocatechin gallate induces apoptosis, via caspase activation, in osteoclasts differentiated from RAW 264.7 cells | - |
dc.type | Article | - |
dc.contributor.college | College of Dentistry (치과대학) | - |
dc.contributor.department | Dept. of Periodontology (치주과학) | - |
dc.contributor.googleauthor | J.-H. Yun | - |
dc.contributor.googleauthor | C.-S. Kim | - |
dc.contributor.googleauthor | S.-H. Choi | - |
dc.contributor.googleauthor | C.-K. Kim | - |
dc.contributor.googleauthor | J.-K. Chai | - |
dc.contributor.googleauthor | K.-S. Cho | - |
dc.identifier.doi | 10.1111/j.1600-0765.2006.00935.x | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A01041 | - |
dc.contributor.localId | A03810 | - |
dc.contributor.localId | A04024 | - |
dc.contributor.localId | A04081 | - |
dc.contributor.localId | A00914 | - |
dc.relation.journalcode | J01696 | - |
dc.identifier.eissn | 1600-0765 | - |
dc.identifier.pmid | 17451540 | - |
dc.identifier.url | http://onlinelibrary.wiley.com/doi/10.1111/j.1600-0765.2006.00935.x/abstract | - |
dc.contributor.alternativeName | Kim, Chong Kwan | - |
dc.contributor.alternativeName | Kim, Chang Sung | - |
dc.contributor.alternativeName | Cho, Kyoo Sung | - |
dc.contributor.alternativeName | Chai, Jung Kyu | - |
dc.contributor.alternativeName | Choi, Seong Ho | - |
dc.contributor.affiliatedAuthor | Kim, Chang Sung | - |
dc.contributor.affiliatedAuthor | Cho, Kyoo Sung | - |
dc.contributor.affiliatedAuthor | Chai, Jung Kyu | - |
dc.contributor.affiliatedAuthor | Choi, Seong Ho | - |
dc.contributor.affiliatedAuthor | Kim, Chong Kwan | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 42 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 212 | - |
dc.citation.endPage | 218 | - |
dc.identifier.bibliographicCitation | JOURNAL OF PERIODONTAL RESEARCH, Vol.42(3) : 212-218, 2007 | - |
dc.identifier.rimsid | 36129 | - |
dc.type.rims | ART | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.