4 562

Cited 32 times in

Spinal AMPA receptor inhibition attenuates mechanical allodynia and neuronal hyperexcitability following spinal cord injury in rats

Authors
 Young Seob Gwak  ;  Jonghoon Kang  ;  Claire E. Hulsebosch  ;  Joong Woo Leem 
Citation
 JOURNAL OF NEUROSCIENCE RESEARCH, Vol.85(11) : 2352-2359, 2007 
Journal Title
JOURNAL OF NEUROSCIENCE RESEARCH
ISSN
 0360-4012 
Issue Date
2007
MeSH
Animals ; Axotomy ; Excitatory Amino Acid Antagonists/administration & dosage* ; Hindlimb/innervation ; Injections, Spinal ; Male ; Neuralgia/drug therapy* ; Neuralgia/etiology ; Neuralgia/physiopathology ; Physical Stimulation ; Posterior Horn Cells/drug effects ; Posterior Horn Cells/metabolism* ; Quinoxalines/administration & dosage* ; Rats ; Rats, Sprague-Dawley ; Receptors, AMPA/antagonists & inhibitors* ; Receptors, AMPA/drug effects ; Recovery of Function/drug effects ; Spinal Cord Injuries/complications ; Spinal Cord Injuries/physiopathology*
Abstract
In this study, we examined whether a competitive AMPA receptor antagonist, NBQX, attenuates mechanical allodynia and hyperexcitability of spinal neurons in remote, caudal regions in persistent central neuropathic pain following spinal cord injury in rats. Spinal cord injury was produced by unilateral T13 transverse spinal hemisection, from dorsal to ventral, in male Sprague Dawley rats (200–250 g). Mechanical thresholds were measured behaviorally, and the excitability of wide-dynamic-range (WDR) dorsal horn neurons in the lumbar cord (L4–L5) was measured to assess central neuropathicpain. On postoperation day (POD) 28 after spinalhemisection, mechanical thresholds were significantly decreased in both injured (ipsilateral) and noninjured (contralateral) hindpaws compared with preinjury and sham control, respectively (P < 0.05). Intrathecal administration of NBQX (0.25, 0.5, 1 mM) significantly reversed the decreased mechanical thresholds in both hindpaws, dose dependently (P < 0.05). The excitability of WDR neurons was significantly enhanced on both sides of the lumbar dorsal horn 28 days following spinal hemisection (P < 0.05). The hyperexcitability of WDR neurons was attenuated by topical administration of NBQX (0.125, 0.25, 0.5, 1 mM), dose dependently (P < 0.05). Regression analysis indicated that at least three molecules of NBQX bond per receptor complex, and are needed to achieve inhibition of WDR hyperexcitability. In conclusion, our study demonstrates that the AMPA receptor plays an important role in behaviors related to the maintenance of central neuropathic pain below the level of spinal cord injury.
Full Text
http://onlinelibrary.wiley.com/doi/10.1002/jnr.21379/abstract
DOI
10.1002/jnr.21379
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Physiology (생리학교실) > 1. Journal Papers
Yonsei Authors
Gwak, Young Seob(곽영섭)
Leem, Joong Woo(임중우) ORCID logo https://orcid.org/0000-0002-1605-2230
Jang, Jun Ho(장준호)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/96489
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse

Links