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Improved Detection of Mycobacterium leprae by One-tube Nested Polymerase Chain Reaction
DC Field | Value | Language |
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dc.contributor.author | 조상래 | - |
dc.date.accessioned | 2014-12-21T16:46:43Z | - |
dc.date.available | 2014-12-21T16:46:43Z | - |
dc.date.issued | 2007 | - |
dc.identifier.issn | 1738-3226 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/96333 | - |
dc.description.abstract | One-tube nested polymerase chain reaction (PCR) was evaluated for its efficacy in detecting Mycobacterium leprae in biopsy samples from leprosy patients. Primers were derived from the M. leprae-specific element (RLEP) sequences which yield a 230 bp fragment. The specificity and the sensitivity of the one-tube nested PCR were compared with those of single PCR for detecting M. leprae. The results showed that the one-tube nested PCR was about 100 times more sensitive than that of the single indicating the one-tube nested primer sets developed in this study can be an effective screening tool for the detection of M. leprae in clinical diagnostic laboratories. | - |
dc.description.statementOfResponsibility | open | - |
dc.format | application/pdf | - |
dc.relation.isPartOf | Journal of Experimental & Biomedcal Science (대한의생명과학회지) | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | Improved Detection of Mycobacterium leprae by One-tube Nested Polymerase Chain Reaction | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Microbiology (미생물학) | - |
dc.contributor.googleauthor | Hye-Young Wang | - |
dc.contributor.googleauthor | Joo-Hwan Whang | - |
dc.contributor.googleauthor | Sang-Nae Cho | - |
dc.contributor.googleauthor | Hyeyoung Lee | - |
dc.contributor.googleauthor | Hyeeun Bang | - |
dc.contributor.googleauthor | Jang-Eun Cho | - |
dc.contributor.googleauthor | Jong-Pill Kim | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A03824 | - |
dc.relation.journalcode | J01408 | - |
dc.contributor.alternativeName | Cho, Sang Nae | - |
dc.contributor.affiliatedAuthor | Cho, Sang Nae | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 13 | - |
dc.citation.number | 4 | - |
dc.citation.startPage | 319 | - |
dc.citation.endPage | 324 | - |
dc.identifier.bibliographicCitation | Journal of Experimental & Biomedcal Science (대한의생명과학회지), Vol.13(4) : 319-324, 2007 | - |
dc.identifier.rimsid | 37018 | - |
dc.type.rims | ART | - |
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