Human leukocyte antigen-G ; Interleukin-10 ; Cervical carcinoma
Abstract
OBJECTIVE:
To determine human leukocyte antigen (HLA)-G and interleukin (IL)-10 expression and the relationship between HLA-G expression and clinicopathologic features in patients with cervical cancer.
METHOD:
Tissue samples were obtained from 40 patients with cervical cancer and 15 control patients with a normal cervix. Quantitative real-time RT-PCR for mRNA and western blot analysis for protein expression were used.
RESULT:
Both HLA-G and IL-10 mRNA expression in cervical cancer tissues was significantly greater than normal controls (P<0.001, P=0.005). Protein expression of HLA-G and IL-10 in the cancer group was also significantly greater than in the controls (P<0.001, P=0.021). There was an inverse relationship between FIGO stage and HLA-G mRNA expression (P=0.046).
CONCLUSION:
HLA-G and IL-10 might play an important role in cancer progression of the cervix. High HLA-G mRNA expression may be related to early carcinogenesis since it was associated with early-stage cervical cancer.