Cited 46 times in
Multi-layered culture of primary human conjunctival epithelial cells producing MUC5AC
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 이형근 | - |
dc.contributor.author | 서경률 | - |
dc.contributor.author | 윤주헌 | - |
dc.date.accessioned | 2014-12-21T16:37:00Z | - |
dc.date.available | 2014-12-21T16:37:00Z | - |
dc.date.issued | 2007 | - |
dc.identifier.issn | 0014-4835 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/96024 | - |
dc.description.abstract | The purpose of our study was to establish a system for culturing normal human conjunctival epithelial (NHCE) cells under serum-free culture conditions without compromising their ability to differentiate into a mucous epithelium. To this end, small pieces of normal conjunctiva were biopsied from patients undergoing cataract surgery. Obtained NHCE cells were cultured in bronchial epithelial growth medium (BEGM) under serum free culture conditions and passage 3 cells were air-lifted. Cultured NHCE cells displayed typical epithelial morphology. Expression of cytokeratin 19 and conjunctival epithelial specific carbohydrate residue were detected. Air-lifted NHCE cells demonstrated an increase in stratification and differentiation into goblet cells up to 3 weeks under air-liquid interface (ALI) culture condition. NHCE cells expressed MUC1, MUC4, MUC16, and MUC5AC mRNA, and MUC5AC production and secretion increased in a time dependent manner after culture under ALI conditions. Exposure of cells to proinflammatory cytokines (TNF-α or IFN-γ) resulted in upregulation of MUC1, MUC4, MUC16, and MUC5AC gene expression. In conclusion, differentiated NHCE cells showed features of a multi-layered conjunctival epithelium, including goblet cells, and retained functional characteristics similar to those seen in vivo. This cell culture system can better facilitate investigation of conjunctival epithelial cell biology and goblet cell differentiation. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 226~233 | - |
dc.relation.isPartOf | EXPERIMENTAL EYE RESEARCH | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Adult | - |
dc.subject.MESH | Aged | - |
dc.subject.MESH | CA-125 Antigen/biosynthesis | - |
dc.subject.MESH | CA-125 Antigen/genetics | - |
dc.subject.MESH | Cell Culture Techniques/methods | - |
dc.subject.MESH | Cell Differentiation | - |
dc.subject.MESH | Cell Proliferation | - |
dc.subject.MESH | Cell Size | - |
dc.subject.MESH | Conjunctiva/cytology* | - |
dc.subject.MESH | Conjunctiva/metabolism | - |
dc.subject.MESH | Conjunctiva/ultrastructure | - |
dc.subject.MESH | Cytokines/pharmacology | - |
dc.subject.MESH | Epithelial Cells/metabolism | - |
dc.subject.MESH | Epithelial Cells/ultrastructure | - |
dc.subject.MESH | Eye Proteins/biosynthesis* | - |
dc.subject.MESH | Female | - |
dc.subject.MESH | Gene Expression Regulation/drug effects | - |
dc.subject.MESH | Goblet Cells/cytology | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Inflammation Mediators/pharmacology | - |
dc.subject.MESH | Male | - |
dc.subject.MESH | Membrane Proteins/biosynthesis | - |
dc.subject.MESH | Membrane Proteins/genetics | - |
dc.subject.MESH | Middle Aged | - |
dc.subject.MESH | Mucin 5AC | - |
dc.subject.MESH | Mucin-1/biosynthesis | - |
dc.subject.MESH | Mucin-1/genetics | - |
dc.subject.MESH | Mucins/biosynthesis* | - |
dc.subject.MESH | Mucins/genetics | - |
dc.subject.MESH | RNA, Messenger/genetics | - |
dc.title | Multi-layered culture of primary human conjunctival epithelial cells producing MUC5AC | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Ophthalmology (안과학) | - |
dc.contributor.googleauthor | So-Hyang Chung | - |
dc.contributor.googleauthor | Joon H. Lee | - |
dc.contributor.googleauthor | Kyoung Yul Seo | - |
dc.contributor.googleauthor | Hyung Keun Lee | - |
dc.contributor.googleauthor | Joo-Heon Yoon | - |
dc.identifier.doi | 10.1016/j.exer.2007.04.005 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A03303 | - |
dc.contributor.localId | A01870 | - |
dc.contributor.localId | A02604 | - |
dc.relation.journalcode | J00868 | - |
dc.identifier.eissn | 1096-0007 | - |
dc.identifier.pmid | 17568580 | - |
dc.identifier.url | http://www.sciencedirect.com/science/article/pii/S001448350700111X | - |
dc.contributor.alternativeName | Lee, Hyung Keun | - |
dc.contributor.alternativeName | Seo, Kyuong Yul | - |
dc.contributor.alternativeName | Yoon, Joo Heon | - |
dc.contributor.affiliatedAuthor | Lee, Hyung Keun | - |
dc.contributor.affiliatedAuthor | Seo, Kyuong Yul | - |
dc.contributor.affiliatedAuthor | Yoon, Joo Heon | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 85 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 226 | - |
dc.citation.endPage | 233 | - |
dc.identifier.bibliographicCitation | EXPERIMENTAL EYE RESEARCH, Vol.85(2) : 226-233, 2007 | - |
dc.identifier.rimsid | 35378 | - |
dc.type.rims | ART | - |
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