3 220

Cited 31 times in

Nuclear localization signals of the E-cadherin transcriptional repressor snail

 Hyeonseok Ko  ;  Hyun Sil Kim  ;  Jong In Yook  ;  Su Hyung Hong  ;  Kun Hong Kim  ;  Sang Hwy Lee  ;  Nam Hee Kim 
 Cells Tissues Organs, Vol.185(1-3) : 66-72, 2007 
Journal Title
 Cells Tissues Organs 
Issue Date
The E-cadherin transcriptional repressor, Snail, plays a critical role in driving the epithelial-mesenchymal transition programs that mark gastrulation as well as invasion of cancer cells. Recent data suggest that Snail is phosphorylated by GSK3-β, resulting in β-TRCP-mediated ubiquitination and proteasomal degradation. Accordingly, Wnt signaling inhibits Snail phosphorylation, and consequently increases Snail protein levels. In the present study, we examine the function of nuclear localization motifs embedded within the Snail sequence. A typical bipartite nuclear localization signal (NLS) motif is located at the N-terminal of Snail, where it overlaps with the SNAG domain (residues 8–16), while a basic cluster NLS motif is found proximal to zinc finger domains (residues 151–152). Mutational inactivation of these NLS signals resulted in decreased levels of nuclear and total Snail protein as well as attenuated Snail repressor activity on an E-cadherin promoter construct, suggesting that NLS motifs are essential for proper function. In the presence of GSK3 inhibitor LiCl, the cytoplasmic levels of the NLS mutants increased, suggesting that cytosolic Snail undergoes rapid phosphorylation and degradation. Given the highly conserved nature of the Snail NLS motifs (from Xenopus to human), these results indicate that nuclear localization signals regulate Snail expression and subcellular localization via GSK3-β-dependent phosphorylation.
Full Text
Appears in Collections:
5. Research Institutes (연구소) > Oral Cancer Research Institute (구강종양연구소) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Oral and Maxillofacial Surgery (구강악안면외과학교실) > 1. Journal Papers
2. College of Dentistry (치과대학) > Dept. of Oral Pathology (구강병리학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Biochemistry and Molecular Biology (생화학-분자생물학교실) > 1. Journal Papers
Yonsei Authors
김건홍(Kim, Kun Hong) ORCID logo https://orcid.org/0000-0001-5639-6372
김남희(Kim, Nam Hee) ORCID logo https://orcid.org/0000-0002-3087-5276
김현실(Kim, Hyun Sil) ORCID logo https://orcid.org/0000-0003-3614-1764
육종인(Yook, Jong In) ORCID logo https://orcid.org/0000-0002-7318-6112
이상휘(Lee, Sang Hwy) ORCID logo https://orcid.org/0000-0002-9438-2489
RIS (EndNote)
XLS (Excel)
사서에게 알리기


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.