Cited 344 times in
Nano-fibrous scaffolding promotes osteoblast differentiation and biomineralization
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 전지혜 | - |
dc.date.accessioned | 2014-12-21T16:26:50Z | - |
dc.date.available | 2014-12-21T16:26:50Z | - |
dc.date.issued | 2007 | - |
dc.identifier.issn | 0142-9612 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/95709 | - |
dc.description.abstract | Nano-fibrous poly(l-lactic acid) (PLLA) scaffolds with interconnected pores were developed under the hypothesis that nano-fibrous scaffolding would mimic a morphological function of collagen fibrils to create a more favorable microenvironment for cells versus solid-walled scaffolds. In this study, an in vitro system was used to examine biological properties of the nano-fibrous scaffolds compared with those of solid-walled scaffolds for their potential use in bone tissue engineering. Biomineralization was enhanced substantially on the nano-fibous scaffolds compared to solid-walled scaffolds, and this was confirmed by von Kossa staining, measurement of calcium contents, and transmission electron microscopy. In support of this finding, osteoblasts cultured on the nano-fibrous scaffolds exhibited higher alkaline phosphatase activity and an earlier and enhanced expression of the osteoblast phenotype versus solid-walled scaffolds. Most notable were the increases in runx2 protein and in bone sialoprotein mRNA in cells cultured on nano-fibrous scaffolds versus solid-walled scaffolds. At the day 1 of culture, α2 and β1 integrins as well as αv and β3 integrins were highly expressed on the surface of cells seeded on nano-fibrous scaffolds, and linked to this were higher levels of phospho-Paxillin and phospho-FAK in cell lysates. In contrast, cells seeded on solid-walled scaffolds expressed significantly lower levels of these integrins, phospho-Paxillin, and phospho-FAK. To further examine the role of nano-fibrous architecture, we inhibited the formation of collagen fibrils by adding 3,4-dehydroproline to cultures and then assayed cells for expression of α2 integrin. Cells seeded on nano-fibrous scaffolds sustained expression of α2 integrin in the presence of dehydroproline, while suppression of α2 integrin was evident in cells seeded on solid-walled scaffolds. These results provide initial evidence that synthetic nano fibers may exhibit certain properties that are comparable to natural collagen fibers, and thus, the nano-fibrous architecture may serve as a superior scaffolding versus solid-walled architecture for promoting osteoblast differentiation and biomineralization. | - |
dc.description.statementOfResponsibility | open | - |
dc.relation.isPartOf | BIOMATERIALS | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | Nano-fibrous scaffolding promotes osteoblast differentiation and biomineralization | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Life Science (의생명과학부) | - |
dc.contributor.googleauthor | Kyung Mi Woo | - |
dc.contributor.googleauthor | Ji-Hae Jun | - |
dc.contributor.googleauthor | Peter X. Ma | - |
dc.contributor.googleauthor | Martha J. Somerman | - |
dc.contributor.googleauthor | Gwan-Shik Kim | - |
dc.contributor.googleauthor | Hyun-Mo Ryoo | - |
dc.contributor.googleauthor | Jeong-Hwa Baek | - |
dc.contributor.googleauthor | Jihye Seo | - |
dc.contributor.googleauthor | Victor J. Chen | - |
dc.identifier.doi | 10.1016/j.biomaterials.2006.06.013 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A03551 | - |
dc.relation.journalcode | J00312 | - |
dc.identifier.eissn | 1878-5905 | - |
dc.identifier.url | http://www.sciencedirect.com/science/article/pii/S0142961206005680 | - |
dc.contributor.alternativeName | Jun, Ji Hae | - |
dc.contributor.affiliatedAuthor | Jun, Ji Hae | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 28 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | 335 | - |
dc.citation.endPage | 343 | - |
dc.identifier.bibliographicCitation | BIOMATERIALS, Vol.28(2) : 335-343, 2007 | - |
dc.identifier.rimsid | 45038 | - |
dc.type.rims | ART | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.