2 136

Cited 17 times in

Upstream stimulatory factors regulate the C/EBPα gene during differentiation of 3T3-L1 preadipocytes

Authors
 Jae-woo Kim  ;  Henrik Monila  ;  M. Daniel Lane  ;  Akhilesh Pandey 
Citation
 Biochemical and Biophysical Research Communications, Vol.354(2) : 517-521, 2007 
Journal Title
 Biochemical and Biophysical Research Communications 
ISSN
 0006-291X 
Issue Date
2007
Abstract
During adipocyte differentiation, CCAAT/enhancer-binding protein α (C/EBPα) functions as a pleiotropic transcriptional activator of numerous adipocyte genes. The promoter of the C/EBPα gene has an E-box upstream of C/EBP binding site. Deletion or mutation of the E-box decreases promoter activity, suggesting that the E-box participates in the regulation of C/EBPα expression. Protein binding to the E-box during the adipocyte differentiation is increased as indicated by EMSA and UV cross-linking. Purification of the E-box binding proteins from differentiated 3T3-L1 adipocytes, showed that USF and AP-4 are associated with the E-box. Supershift analysis showed that USF1 and USF2 bind to this element as heterodimers, whereas the addition of anti-AP-4 antibody enhanced the binding complex, suggesting that AP-4 negatively regulates the promoter activity. The expression of AP-4 is reciprocally regulated with USF-1 during adipocyte differentiation. These findings suggest that USF-1 and 2 play roles in C/EBPα expression, whereas the AP-4 represses it.
Full Text
http://www.sciencedirect.com/science/article/pii/S0006291X07000393
DOI
10.1016/j.bbrc.2007.01.008
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Biochemistry and Molecular Biology (생화학-분자생물학교실) > 1. Journal Papers
Yonsei Authors
김재우(Kim, Jae Woo) ORCID logo https://orcid.org/0000-0001-5456-9495
Export
RIS (EndNote)
XLS (Excel)
XML
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/95688
사서에게 알리기
  feedback

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse