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Application of the 16-kDa buckwheat 2 S storage albumin protein for diagnosis of clinical reactivity

DC FieldValueLanguage
dc.contributor.author박중원-
dc.contributor.author손정호-
dc.contributor.author이용원-
dc.contributor.author이은경-
dc.contributor.author최수영-
dc.contributor.author홍천수-
dc.date.accessioned2014-12-21T16:23:36Z-
dc.date.available2014-12-21T16:23:36Z-
dc.date.issued2007-
dc.identifier.issn1081-1206-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/95608-
dc.description.abstractBACKGROUND: The 16-kDa protein of buckwheat (BW) has been implicated as a major allergen in BW allergy. OBJECTIVE: To characterize the 16-kDa allergen and evaluate its clinical significance as an indicator of BW allergy. METHODS: Complementary DNA from the 16-kDa allergen was cloned and expressed in Escherichia coli. Allergenicity was confirmed with IgE immunoblotting or with an enzyme-linked immunosorbent assay. The clinical utility of the recombinant protein (r16 kDa) for diagnosis of BW reactivity was evaluated in 18 BW-allergic and in 20 asymptomatic BW-sensitized subjects. RESULTS: The 16-kDa allergen, composed of 127 amino acids, has 50% homology to the reported 8-kDa BW allergen, which belongs to the 2 S storage albumin. The r16-kDa protein can inhibit specific IgE (sIgE) antibody binding to the native BW 16-kDa allergen but minimally inhibited sIgE binding to crude BW extract. Approximately 77.8% of patients with the BW allergy produced sIgE antibodies to the r16-kDa protein, compared with a complete lack of reactivity in the 20 asymptomatic BW-sensitized subjects. The areas of the receiver operating characteristic curves for the skin prick test (mean, 0.93; 95% confidence interval, 0.85 to approximately 1.01; P < .001) and the rl6-kDa enzyme-linked immunosorbent assay (mean, 0.93; 95% confidence interval, 0.84 to approximately 1.01; P < .001) were higher than the area of the BW IgE measurement curve determined by ImmunoCAP (a system for assaying serum IgE) (mean, 0.80; 95% confidence interval, 0.66 to approximately 0.94; P = .002). CONCLUSIONS: The 16-kDa allergen belongs to the 2 S storage albumin. Measurement of rl6-kDa sIgE was more discriminating than measurement of ImmunoCAP sIgE in whole BW extracts for the diagnosis of clinical reactivity to BW.-
dc.description.statementOfResponsibilityopen-
dc.format.extent254~260-
dc.relation.isPartOfANNALS OF ALLERGY ASTHMA & IMMUNOLOGY-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.titleApplication of the 16-kDa buckwheat 2 S storage albumin protein for diagnosis of clinical reactivity-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentMedical Research Center (임상의학연구센터)-
dc.contributor.googleauthorSoo-Young Choi-
dc.contributor.googleauthorJung-Ho Sohn-
dc.contributor.googleauthorJung-Won Park-
dc.contributor.googleauthorChein-Soo Hong-
dc.contributor.googleauthorEun-Kyung Lee-
dc.contributor.googleauthorYong-Won Lee-
dc.identifier.doi10.1016/S1081-1206(10)60661-8-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA01681-
dc.contributor.localIdA01993-
dc.contributor.localIdA02981-
dc.contributor.localIdA03033-
dc.contributor.localIdA04093-
dc.contributor.localIdA04448-1-
dc.relation.journalcodeJ00153-
dc.identifier.eissn1534-4436-
dc.identifier.urlhttp://www.sciencedirect.com/science/article/pii/S1081120610606618-
dc.contributor.alternativeNamePark, Jung Won-
dc.contributor.alternativeNameSohn, Jung Ho-
dc.contributor.alternativeNameLee, Yong Won-
dc.contributor.alternativeNameLee, Eun Kyung-
dc.contributor.alternativeNameChoi, Soo Young-
dc.contributor.alternativeNameHong, Chein Soo-
dc.contributor.affiliatedAuthorPark, Jung Won-
dc.contributor.affiliatedAuthorSohn, Jung Ho-
dc.contributor.affiliatedAuthorLee, Yong Won-
dc.contributor.affiliatedAuthorLee, Eun Kyung-
dc.contributor.affiliatedAuthorChoi, Soo Young-
dc.contributor.affiliatedAuthorHong, Chein Soo-
dc.rights.accessRightsnot free-
dc.citation.volume99-
dc.citation.number3-
dc.citation.startPage254-
dc.citation.endPage260-
dc.identifier.bibliographicCitationANNALS OF ALLERGY ASTHMA & IMMUNOLOGY, Vol.99(3) : 254-260, 2007-
Appears in Collections:
1. College of Medicine (의과대학) > Medical Research Center (임상의학연구센터) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers

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