Cited 15 times in
Application of the 16-kDa buckwheat 2 S storage albumin protein for diagnosis of clinical reactivity
DC Field | Value | Language |
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dc.contributor.author | 박중원 | - |
dc.contributor.author | 손정호 | - |
dc.contributor.author | 이용원 | - |
dc.contributor.author | 이은경 | - |
dc.contributor.author | 최수영 | - |
dc.contributor.author | 홍천수 | - |
dc.date.accessioned | 2014-12-21T16:23:36Z | - |
dc.date.available | 2014-12-21T16:23:36Z | - |
dc.date.issued | 2007 | - |
dc.identifier.issn | 1081-1206 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/95608 | - |
dc.description.abstract | BACKGROUND: The 16-kDa protein of buckwheat (BW) has been implicated as a major allergen in BW allergy. OBJECTIVE: To characterize the 16-kDa allergen and evaluate its clinical significance as an indicator of BW allergy. METHODS: Complementary DNA from the 16-kDa allergen was cloned and expressed in Escherichia coli. Allergenicity was confirmed with IgE immunoblotting or with an enzyme-linked immunosorbent assay. The clinical utility of the recombinant protein (r16 kDa) for diagnosis of BW reactivity was evaluated in 18 BW-allergic and in 20 asymptomatic BW-sensitized subjects. RESULTS: The 16-kDa allergen, composed of 127 amino acids, has 50% homology to the reported 8-kDa BW allergen, which belongs to the 2 S storage albumin. The r16-kDa protein can inhibit specific IgE (sIgE) antibody binding to the native BW 16-kDa allergen but minimally inhibited sIgE binding to crude BW extract. Approximately 77.8% of patients with the BW allergy produced sIgE antibodies to the r16-kDa protein, compared with a complete lack of reactivity in the 20 asymptomatic BW-sensitized subjects. The areas of the receiver operating characteristic curves for the skin prick test (mean, 0.93; 95% confidence interval, 0.85 to approximately 1.01; P < .001) and the rl6-kDa enzyme-linked immunosorbent assay (mean, 0.93; 95% confidence interval, 0.84 to approximately 1.01; P < .001) were higher than the area of the BW IgE measurement curve determined by ImmunoCAP (a system for assaying serum IgE) (mean, 0.80; 95% confidence interval, 0.66 to approximately 0.94; P = .002). CONCLUSIONS: The 16-kDa allergen belongs to the 2 S storage albumin. Measurement of rl6-kDa sIgE was more discriminating than measurement of ImmunoCAP sIgE in whole BW extracts for the diagnosis of clinical reactivity to BW. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 254~260 | - |
dc.relation.isPartOf | ANNALS OF ALLERGY ASTHMA & IMMUNOLOGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | Application of the 16-kDa buckwheat 2 S storage albumin protein for diagnosis of clinical reactivity | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Medical Research Center (임상의학연구센터) | - |
dc.contributor.googleauthor | Soo-Young Choi | - |
dc.contributor.googleauthor | Jung-Ho Sohn | - |
dc.contributor.googleauthor | Jung-Won Park | - |
dc.contributor.googleauthor | Chein-Soo Hong | - |
dc.contributor.googleauthor | Eun-Kyung Lee | - |
dc.contributor.googleauthor | Yong-Won Lee | - |
dc.identifier.doi | 10.1016/S1081-1206(10)60661-8 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A01681 | - |
dc.contributor.localId | A01993 | - |
dc.contributor.localId | A02981 | - |
dc.contributor.localId | A03033 | - |
dc.contributor.localId | A04093 | - |
dc.contributor.localId | A04448-1 | - |
dc.relation.journalcode | J00153 | - |
dc.identifier.eissn | 1534-4436 | - |
dc.identifier.url | http://www.sciencedirect.com/science/article/pii/S1081120610606618 | - |
dc.contributor.alternativeName | Park, Jung Won | - |
dc.contributor.alternativeName | Sohn, Jung Ho | - |
dc.contributor.alternativeName | Lee, Yong Won | - |
dc.contributor.alternativeName | Lee, Eun Kyung | - |
dc.contributor.alternativeName | Choi, Soo Young | - |
dc.contributor.alternativeName | Hong, Chein Soo | - |
dc.contributor.affiliatedAuthor | Park, Jung Won | - |
dc.contributor.affiliatedAuthor | Sohn, Jung Ho | - |
dc.contributor.affiliatedAuthor | Lee, Yong Won | - |
dc.contributor.affiliatedAuthor | Lee, Eun Kyung | - |
dc.contributor.affiliatedAuthor | Choi, Soo Young | - |
dc.contributor.affiliatedAuthor | Hong, Chein Soo | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 99 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 254 | - |
dc.citation.endPage | 260 | - |
dc.identifier.bibliographicCitation | ANNALS OF ALLERGY ASTHMA & IMMUNOLOGY, Vol.99(3) : 254-260, 2007 | - |
dc.identifier.rimsid | 44961 | - |
dc.type.rims | ART | - |
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