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Effect of Slc26a6 deletion on apical Cl−/HCO3− exchanger activity and cAMP-stimulated bicarbonate secretion in pancreatic duct

DC FieldValueLanguage
dc.contributor.author이민구-
dc.date.accessioned2014-12-21T16:22:08Z-
dc.date.available2014-12-21T16:22:08Z-
dc.date.issued2007-
dc.identifier.issn0193-1857-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/95561-
dc.description.abstractThe role of Slc26a6 (PAT1) on apical Cl-/HCO3- exchange and bicarbonate secretion in pancreatic duct cells was investigated using Slc26a6 null and wild-type (WT) mice. Apical Cl-/HCO3- exchange activity was measured with the pH-sensitive dye BCECF in microperfused interlobular ducts. The HCO3(-)-influx mode of apical [Cl-]i/[HCO3-]o exchange (where brackets denote concentration and subscripts i and o denote intra- and extracellular, respectively) was dramatically upregulated in Slc26a6 null mice (P < 0.01 vs. WT), whereas the HCO3(-)-efflux mode of apical [Cl-]o/[HCO3-]i exchange was decreased in Slc26a6 null mice (P < 0.05 vs. WT), suggesting the unidirectionality of the Slc26a6-mediated HCO3- transport. Fluid secretory rate in interlobular ducts were comparable in WT and Slc26a6 null mice (P > 0.05). In addition, when pancreatic juice was collected from whole animal in basal and secretin-stimulated conditions, neither juice volume nor its pH showed differences between WT and Slc26a6 null mice. Semiquantitative RT-PCR demonstrated more than fivefold upregulation in Slc26a3 (DRA) expression in Slc26a6 knockout pancreas. In conclusion, these results point to the role of Slc26a6 in HCO3- efflux at the apical membrane and also suggest the presence of a robust Slc26a3 compensatory upregulation, which can replace the function of Slc26a6 in pancreatic ducts.-
dc.description.statementOfResponsibilityopen-
dc.format.extentG447~G455-
dc.relation.isPartOfAMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.titleEffect of Slc26a6 deletion on apical Cl−/HCO3− exchanger activity and cAMP-stimulated bicarbonate secretion in pancreatic duct-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Pharmacology (약리학)-
dc.contributor.googleauthorHiroshi Ishiguro-
dc.contributor.googleauthorWan Namkung-
dc.contributor.googleauthorManoocher Soleimani-
dc.contributor.googleauthorMin Goo Lee-
dc.contributor.googleauthorJie Xu-
dc.contributor.googleauthorRoger T. Worrell-
dc.contributor.googleauthorZhaohui Wang-
dc.contributor.googleauthorAkiko Yamamoto-
dc.identifier.doi10.1152/ajpgi.00286.2006-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA02781-
dc.relation.journalcodeJ00104-
dc.identifier.eissn1522-1547-
dc.contributor.alternativeNameLee, Min Goo-
dc.contributor.affiliatedAuthorLee, Min Goo-
dc.rights.accessRightsfree-
dc.citation.volume292-
dc.citation.number1-
dc.citation.startPage447-
dc.citation.endPage455-
dc.identifier.bibliographicCitationAMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY, Vol.292(1) : 447-455, 2007-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Pharmacology (약리학교실) > 1. Journal Papers

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