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Effect of Slc26a6 deletion on apical Cl−/HCO3− exchanger activity and cAMP-stimulated bicarbonate secretion in pancreatic duct
DC Field | Value | Language |
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dc.contributor.author | 이민구 | - |
dc.date.accessioned | 2014-12-21T16:22:08Z | - |
dc.date.available | 2014-12-21T16:22:08Z | - |
dc.date.issued | 2007 | - |
dc.identifier.issn | 0193-1857 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/95561 | - |
dc.description.abstract | The role of Slc26a6 (PAT1) on apical Cl-/HCO3- exchange and bicarbonate secretion in pancreatic duct cells was investigated using Slc26a6 null and wild-type (WT) mice. Apical Cl-/HCO3- exchange activity was measured with the pH-sensitive dye BCECF in microperfused interlobular ducts. The HCO3(-)-influx mode of apical [Cl-]i/[HCO3-]o exchange (where brackets denote concentration and subscripts i and o denote intra- and extracellular, respectively) was dramatically upregulated in Slc26a6 null mice (P < 0.01 vs. WT), whereas the HCO3(-)-efflux mode of apical [Cl-]o/[HCO3-]i exchange was decreased in Slc26a6 null mice (P < 0.05 vs. WT), suggesting the unidirectionality of the Slc26a6-mediated HCO3- transport. Fluid secretory rate in interlobular ducts were comparable in WT and Slc26a6 null mice (P > 0.05). In addition, when pancreatic juice was collected from whole animal in basal and secretin-stimulated conditions, neither juice volume nor its pH showed differences between WT and Slc26a6 null mice. Semiquantitative RT-PCR demonstrated more than fivefold upregulation in Slc26a3 (DRA) expression in Slc26a6 knockout pancreas. In conclusion, these results point to the role of Slc26a6 in HCO3- efflux at the apical membrane and also suggest the presence of a robust Slc26a3 compensatory upregulation, which can replace the function of Slc26a6 in pancreatic ducts. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | G447~G455 | - |
dc.relation.isPartOf | AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | Effect of Slc26a6 deletion on apical Cl−/HCO3− exchanger activity and cAMP-stimulated bicarbonate secretion in pancreatic duct | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Pharmacology (약리학) | - |
dc.contributor.googleauthor | Hiroshi Ishiguro | - |
dc.contributor.googleauthor | Wan Namkung | - |
dc.contributor.googleauthor | Manoocher Soleimani | - |
dc.contributor.googleauthor | Min Goo Lee | - |
dc.contributor.googleauthor | Jie Xu | - |
dc.contributor.googleauthor | Roger T. Worrell | - |
dc.contributor.googleauthor | Zhaohui Wang | - |
dc.contributor.googleauthor | Akiko Yamamoto | - |
dc.identifier.doi | 10.1152/ajpgi.00286.2006 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A02781 | - |
dc.relation.journalcode | J00104 | - |
dc.identifier.eissn | 1522-1547 | - |
dc.contributor.alternativeName | Lee, Min Goo | - |
dc.contributor.affiliatedAuthor | Lee, Min Goo | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 292 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 447 | - |
dc.citation.endPage | 455 | - |
dc.identifier.bibliographicCitation | AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY, Vol.292(1) : 447-455, 2007 | - |
dc.identifier.rimsid | 43333 | - |
dc.type.rims | ART | - |
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