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Inhibitory effects of broccoli extract on Escherichia coli O157:H7 quorum sensing and in vivo virulence.

DC Field Value Language
dc.contributor.author박용진-
dc.contributor.author윤상선-
dc.contributor.author이강무-
dc.date.accessioned2014-12-20T17:45:48Z-
dc.date.available2014-12-20T17:45:48Z-
dc.date.issued2011-
dc.identifier.issn0378-1097-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/95215-
dc.description.abstractBroccoli extract (BE) has numerous beneficial effects on human health including anticancer activity. Quorum sensing (QS), mediated by self-produced autoinducer (AI) molecules, is a key process for the production of virulence determinants in pathogenic bacteria. BE suppressed AI-2 synthesis and AI-2-mediated bacterial motility in a dose-dependent manner in Escherichia coli O157:H7. In addition, expression of the ler gene that regulates AI-3 QS system was also diminished in response to treatment with BE. Furthermore, in an in vivo efficacy test using Caenorhabditis elegans as a host organism, C. elegans fed on E. coli O157:H7 in the presence of BE survived longer than those fed solely on the pathogenic bacteria. Quantitative real-time PCR analysis indicated that quercetin was the most active among the tested broccoli-derived compounds in downregulating virulence gene expression, while treatment with myricetin significantly suppressed the expression of the eae gene involved in type III secretion system. These data suggest that BE and its flavonoid constituents can inhibit expression of QS-associated genes, thereby downregulating the virulence attributes of E. coli O157:H7 both in vitro and in vivo. This study clearly elucidates BE's QS-inhibitory activity and suggests that BE has the potential to be developed as an anti-infective agent.-
dc.description.statementOfResponsibilityopen-
dc.format.extent67~74-
dc.relation.isPartOfFEMS MICROBIOLOGY LETTERS-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAdrenergic alpha-Agonists/pharmacology-
dc.subject.MESHAnimals-
dc.subject.MESHAnti-Bacterial Agents/pharmacology*-
dc.subject.MESHBrassica/chemistry*-
dc.subject.MESHCaenorhabditis elegans/microbiology*-
dc.subject.MESHDown-Regulation/drug effects-
dc.subject.MESHEscherichia coli O157/drug effects*-
dc.subject.MESHEscherichia coli O157/genetics-
dc.subject.MESHEscherichia coli O157/pathogenicity*-
dc.subject.MESHEscherichia coli Proteins/genetics-
dc.subject.MESHEscherichia coli Proteins/metabolism-
dc.subject.MESHFlavonoids/pharmacology-
dc.subject.MESHGene Expression Regulation, Bacterial/drug effects-
dc.subject.MESHHomoserine/analogs & derivatives-
dc.subject.MESHHomoserine/genetics-
dc.subject.MESHHomoserine/metabolism-
dc.subject.MESHLactones/metabolism-
dc.subject.MESHNorepinephrine/pharmacology-
dc.subject.MESHPlant Extracts/pharmacology*-
dc.subject.MESHPromoter Regions, Genetic/genetics-
dc.subject.MESHQuorum Sensing/drug effects*-
dc.subject.MESHQuorum Sensing/genetics-
dc.subject.MESHTrans-Activators/genetics-
dc.subject.MESHTrans-Activators/metabolism-
dc.subject.MESHVirulence/drug effects-
dc.titleInhibitory effects of broccoli extract on Escherichia coli O157:H7 quorum sensing and in vivo virulence.-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Microbiology (미생물학)-
dc.contributor.googleauthorKang-Mu Lee-
dc.contributor.googleauthorJeesun Lim-
dc.contributor.googleauthorSunyoung Nam-
dc.contributor.googleauthorMi Young Yoon-
dc.contributor.googleauthorYong-Kuk Kwon-
dc.contributor.googleauthorByeong Yeal Jung-
dc.contributor.googleauthorYongJin Park-
dc.contributor.googleauthorSungsu Park-
dc.contributor.googleauthorSang Sun Yoon-
dc.identifier.doi10.1111/j.1574-6968.2011.02311.x-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA02558-
dc.contributor.localIdA02638-
dc.contributor.localIdA01584-
dc.relation.journalcodeJ00892-
dc.identifier.eissn1574-6968-
dc.identifier.pmid21592195-
dc.identifier.urlhttp://onlinelibrary.wiley.com/doi/10.1111/j.1574-6968.2011.02311.x/abstract-
dc.subject.keywordbroccoli extract-
dc.subject.keywordquorum sensing-
dc.subject.keywordEscherichia coli O157:H7-
dc.subject.keywordCaenorhabditis elegans-
dc.contributor.alternativeNamePark, Yong Jin-
dc.contributor.alternativeNameYoon, Sang Sun-
dc.contributor.alternativeNameLee, Kang Mu-
dc.contributor.affiliatedAuthorYoon, Sang Sun-
dc.contributor.affiliatedAuthorLee, Kang Mu-
dc.contributor.affiliatedAuthorPark, Yong Jin-
dc.rights.accessRightsnot free-
dc.citation.volume321-
dc.citation.number1-
dc.citation.startPage67-
dc.citation.endPage74-
dc.identifier.bibliographicCitationFEMS MICROBIOLOGY LETTERS, Vol.321(1) : 67-74, 2011-
dc.identifier.rimsid28182-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Microbiology (미생물학교실) > 1. Journal Papers

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