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Myogenic Differentiation of Human Adipose-Derived Stem Cells
DC Field | Value | Language |
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dc.contributor.author | 박윤길 | - |
dc.contributor.author | 최정화 | - |
dc.date.accessioned | 2014-12-20T17:45:02Z | - |
dc.date.available | 2014-12-20T17:45:02Z | - |
dc.date.issued | 2011 | - |
dc.identifier.issn | 1225-584X | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/95191 | - |
dc.description.abstract | OBJECTIVE: Cell therapy has been extensively studied as a gene complementation approach in muscular dystrophy including Duchenne muscular dystrophy (DMD), and adipose tissue has recently been identified as a uniquely abundant and adequately accessible source of pluripotent cells. In the present work, we investigated myogenic potentials of adipose-derived stem cells (ADSCs) depending on culture media and isolation with using surface markers. METHOD: Human ADSCs were obtained by liposuction and cultured in two different media; control and myogenic media. In addition we attempted to isolate ADSCs by utilizing surface markers: CD45 and CD133. The following observations were made to evaluate myogenic differentiation as the expression of myogenic regulatory factors (MyoD, Myf-5 and Myf-6) and desmin by RT-PCR and immunoflurescence study. RESULTS: Conversion of ADSCs to myogenic phenotype was observed by indirect immunoflurescence study of MyoD and Myf-5 in regardless of media type and isolation method. In addition mRNA of MyoD and Myf-5 were positive in both culture media, and there were no differences of MyoD and Myf-5 responses between CD45- and CD45-CD133-ADSCs. However, secondary myogenic regulatory factor (Myf-6) was not expressed constantly, and desmin were negative in all cultural condition. CONCLUSION: Our findings suggest that human ADSCs might have myogenic potentials. However, further studies are needed to express the secondary myogenic regulatory factors and proteins in myoblasts. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 8~13 | - |
dc.relation.isPartOf | Journal of the Korean Academy of Rehabilitation Medicine (대한재활의학회지) | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | Myogenic Differentiation of Human Adipose-Derived Stem Cells | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Rehabilitation Medicine (재활의학) | - |
dc.contributor.googleauthor | Yoon Ghil Park | - |
dc.contributor.googleauthor | Ah Mi Baek | - |
dc.contributor.googleauthor | Byung Rok Do | - |
dc.contributor.googleauthor | Jung Hwa Choi | - |
dc.contributor.googleauthor | Sun Do Kim | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A01596 | - |
dc.contributor.localId | A04181 | - |
dc.relation.journalcode | J01803 | - |
dc.subject.keyword | Muscular dystrophy | - |
dc.subject.keyword | Stem cell | - |
dc.subject.keyword | Differentiation | - |
dc.subject.keyword | Myogenic regulatory factor | - |
dc.subject.keyword | Desmin | - |
dc.contributor.alternativeName | Park, Yoon Ghil | - |
dc.contributor.alternativeName | Choi, Jung Hwa | - |
dc.contributor.affiliatedAuthor | Park, Yoon Ghil | - |
dc.contributor.affiliatedAuthor | Choi, Jung Hwa | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 35 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 8 | - |
dc.citation.endPage | 13 | - |
dc.identifier.bibliographicCitation | Journal of the Korean Academy of Rehabilitation Medicine (대한재활의학회지), Vol.35(1) : 8-13, 2011 | - |
dc.identifier.rimsid | 28162 | - |
dc.type.rims | ART | - |
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