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Peroxisome proliferator-activated receptor {alpha} is responsible for the up-regulation of hepatic glucose-6-phosphatase gene expression in fasting and db/db Mice.

Authors
 Seung-Soon Im  ;  Mi-Young Kim  ;  Sool-Ki Kwon  ;  Tae-Hyun Kim  ;  Jin-Sik Bae  ;  Hail Kim  ;  Kyung-Sup Kim  ;  Goo-Taeg Oh  ;  Yong-Ho Ahn 
Citation
 JOURNAL OF BIOLOGICAL CHEMISTRY, Vol.286(2) : 1157-1164, 2011 
Journal Title
JOURNAL OF BIOLOGICAL CHEMISTRY
ISSN
 0021-9258 
Issue Date
2011
MeSH
Animals ; Diabetes Mellitus, Type 2/genetics ; Diabetes Mellitus, Type 2/metabolism* ; Disease Models, Animal ; Eating/physiology ; Fasting/physiology ; Gene Expression Regulation, Enzymologic* ; Gluconeogenesis/physiology ; Glucose-6-Phosphatase/genetics* ; Hep G2 Cells ; Humans ; Liver/enzymology ; Male ; Mice ; Mice, Inbred C57BL ; Mice, Mutant Strains ; PPAR alpha/genetics ; PPAR alpha/metabolism* ; Phosphoenolpyruvate Carboxykinase (GTP)/metabolism ; Promoter Regions, Genetic/physiology ; RNA, Messenger/metabolism ; Up-Regulation/physiology
Keywords
Gene Expression ; Gluconeogenesis ; Liver Metabolism ; PPAR ; Transcription ; Glucose-6-phosphatase ; Phosphoenolpyruvate Carboxykinase ; Type 2 Diabetes
Abstract
Glucose-6-phosphatase (G6Pase) is a key enzyme that is responsible for the production of glucose in the liver during fasting or in type 2 diabetes mellitus (T2DM). During fasting or in T2DM, peroxisome proliferator-activated receptor α (PPARα) is activated, which may contribute to increased hepatic glucose output. However, the mechanism by which PPARα up-regulates hepatic G6Pase gene expression in these states is not well understood. We evaluated the mechanism by which PPARα up-regulates hepatic G6Pase gene expression in fasting and T2DM states. In PPARα-null mice, both hepatic G6Pase and phosphoenolpyruvate carboxykinase levels were not increased in the fasting state. Moreover, treatment of primary cultured hepatocytes with Wy14,643 or fenofibrate increased the G6Pase mRNA level. In addition, we have localized and characterized a PPAR-responsive element in the promoter region of the G6Pase gene. Chromatin immunoprecipitation (ChIP) assay revealed that PPARα binding to the putative PPAR-responsive element of the G6Pase promoter was increased in fasted wild-type mice and db/db mice. These results indicate that PPARα is responsible for glucose production through the up-regulation of hepatic G6Pase gene expression during fasting or T2DM animal models.
Files in This Item:
T201104774.pdf Download
DOI
10.1074/jbc.M110.157875
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Biochemistry and Molecular Biology (생화학-분자생물학교실) > 1. Journal Papers
Yonsei Authors
Kwon, Sool Ki(권슬기)
Kim, Kyung Sup(김경섭) ORCID logo https://orcid.org/0000-0001-8483-8537
Kim, Mi Young(김미영)
Kim, Tae Hyun(김태현)
Kim, Ha Il(김하일)
Bae, Jin Sik(배진식)
Ahn, Yong Ho(안용호) ORCID logo https://orcid.org/0000-0002-4133-0757
Im, Seung Soon(임승순)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/94822
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