Cited 28 times in
Proteomic analysis of pancreatic juice for the identification of biomarkers of pancreatic cancer
DC Field | Value | Language |
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dc.contributor.author | 방승민 | - |
dc.contributor.author | 송시영 | - |
dc.contributor.author | 정주원 | - |
dc.contributor.author | 김선아 | - |
dc.contributor.author | 박승우 | - |
dc.contributor.author | 박정엽 | - |
dc.date.accessioned | 2014-12-20T17:30:12Z | - |
dc.date.available | 2014-12-20T17:30:12Z | - |
dc.date.issued | 2011 | - |
dc.identifier.issn | 0171-5216 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/94721 | - |
dc.description.abstract | INTRODUCTION: Protein profiles of endoscopically collected pancreatic juice from normal, chronic pancreatitis patients and pancreatic cancer patients were compared to identify diagnostic biomarkers of pancreatic cancer. METHODS: Secretin was injected intravenously and pancreatic juice was collected via selective cannulation of the pancreatic duct during endoscopic retrograde cholangiopancreatography. Pancreatic juices consisting of three pooled samples for normal control, chronic pancreatitis, and pancreatic cancer patients were compared using two-dimensional gel electrophoresis, and the proteins were subsequently identified using MALDI-TOF-MS. RESULTS: Thirty-five protein spots were up-regulated twofold in pancreatic cancer compared with the levels in the normal controls, and 85 protein spots were present in pancreatic cancer samples but not in normal controls. After excluding spots that were also expressed in chronic pancreatitis, 26 protein spots that were up-regulated or only expressed in pancreatic cancer samples were identified. Among the identified proteins, we confirmed the expressions of BIG2, PRDX6, and REG1α in pancreatic cancer tissue using immunohistochemistry. ELISA showed that the serum level of REG1α was significantly higher in patients with pancreatic cancer than it was in the normal controls (P = 0.023). With the best cut-off value, the sensitivity and specificity of REG1α to differentiate normal and pancreatic cancer were 82.6 and 81.8%, compared with 69.6 and 100% for CA19-9. CONCLUSIONS: We have shown that pancreatic juice is a good source of pancreatic cancer tumor markers. Further studies are needed to determine the clinical implications of REG1α and other markers. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 1229~1238 | - |
dc.relation.isPartOf | JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Adult | - |
dc.subject.MESH | Aged | - |
dc.subject.MESH | Biomarkers, Tumor/analysis* | - |
dc.subject.MESH | Biomarkers, Tumor/blood | - |
dc.subject.MESH | Chi-Square Distribution | - |
dc.subject.MESH | Cholangiopancreatography, Endoscopic Retrograde | - |
dc.subject.MESH | Electrophoresis, Gel, Two-Dimensional | - |
dc.subject.MESH | Enzyme-Linked Immunosorbent Assay | - |
dc.subject.MESH | Female | - |
dc.subject.MESH | Guanine Nucleotide Exchange Factors/analysis | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Immunohistochemistry | - |
dc.subject.MESH | Lithostathine/analysis* | - |
dc.subject.MESH | Lithostathine/blood | - |
dc.subject.MESH | Male | - |
dc.subject.MESH | Middle Aged | - |
dc.subject.MESH | Pancreatic Juice/chemistry* | - |
dc.subject.MESH | Pancreatic Neoplasms/chemistry* | - |
dc.subject.MESH | Pancreatitis/metabolism | - |
dc.subject.MESH | Peroxiredoxin VI/analysis | - |
dc.subject.MESH | Sensitivity and Specificity | - |
dc.subject.MESH | Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization | - |
dc.title | Proteomic analysis of pancreatic juice for the identification of biomarkers of pancreatic cancer | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Internal Medicine (내과학) | - |
dc.contributor.googleauthor | Jeong Youp Park | - |
dc.contributor.googleauthor | Sun-A Kim | - |
dc.contributor.googleauthor | Joo Won Chung | - |
dc.contributor.googleauthor | Seungmin Bang | - |
dc.contributor.googleauthor | Seung Woo Park | - |
dc.contributor.googleauthor | Young-Ki Paik | - |
dc.contributor.googleauthor | Si Young Song | - |
dc.identifier.doi | 10.1007/s00432-011-0992-2 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A01786 | - |
dc.contributor.localId | A02035 | - |
dc.contributor.localId | A03726 | - |
dc.contributor.localId | A00548 | - |
dc.contributor.localId | A01551 | - |
dc.contributor.localId | A01647 | - |
dc.relation.journalcode | J01283 | - |
dc.identifier.eissn | 1432-1335 | - |
dc.identifier.pmid | 21691750 | - |
dc.identifier.url | http://link.springer.com/article/10.1007%2Fs00432-011-0992-2 | - |
dc.subject.keyword | Pancreatic cancer | - |
dc.subject.keyword | Pancreatic juice | - |
dc.subject.keyword | Biomarker | - |
dc.contributor.alternativeName | Bang, Seung Min | - |
dc.contributor.alternativeName | Song, Si Young | - |
dc.contributor.alternativeName | Chung, Joo Won | - |
dc.contributor.alternativeName | Kim, Sun A | - |
dc.contributor.alternativeName | Park, Seung Woo | - |
dc.contributor.alternativeName | Park, Jeong Youp | - |
dc.contributor.affiliatedAuthor | Bang, Seung Min | - |
dc.contributor.affiliatedAuthor | Song, Si Young | - |
dc.contributor.affiliatedAuthor | Chung, Joo Won | - |
dc.contributor.affiliatedAuthor | Kim, Sun A | - |
dc.contributor.affiliatedAuthor | Park, Seung Woo | - |
dc.contributor.affiliatedAuthor | Park, Jeong Youp | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 137 | - |
dc.citation.number | 8 | - |
dc.citation.startPage | 1229 | - |
dc.citation.endPage | 1238 | - |
dc.identifier.bibliographicCitation | JOURNAL OF CANCER RESEARCH AND CLINICAL ONCOLOGY, Vol.137(8) : 1229-1238, 2011 | - |
dc.identifier.rimsid | 27708 | - |
dc.type.rims | ART | - |
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