4 188

Cited 67 times in

A small molecule that binds to an ATPase domain of Hsc70 promotes membrane trafficking of mutant cystic fibrosis transmembrane conductance regulator.

DC FieldValueLanguage
dc.contributor.author이민구-
dc.date.accessioned2014-12-20T17:30:06Z-
dc.date.available2014-12-20T17:30:06Z-
dc.date.issued2011-
dc.identifier.issn0002-7863-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/94718-
dc.description.abstractCystic fibrosis transmembrane conductance regulator (CFTR) is a cell-surface anion channel that permeates chloride and bicarbonate ions. The most frequent mutation of CFTR that causes cystic fibrosis is the deletion of phenylalanine at position 508 (ΔF508), which leads to defects in protein folding and cellular trafficking to the plasma membrane. The lack of the cell-surface CFTR results in a reduction in the lifespan due to chronic lung infection with progressive deterioration of lung function. Hsc70 plays a crucial role in degradation of mutant CFTR by the ubiquitin-proteasome system. To date, various Hsc70 inhibitors and transcription regulators have been tested to determine whether they correct the defective activity of mutant CFTR. However, they exhibited limited or questionable effects on restoring the chloride channel activity in cystic fibrosis cells. Herein, we show that a small molecule apoptozole (Az) has high cellular potency to promote membrane trafficking of mutant CFTR and its chloride channel activity in cystic fibrosis cells. Results from affinity chromatography and ATPase activity assay indicate that Az inhibits the ATPase activity of Hsc70 by binding to its ATPase domain. In addition, a ligand-directed protein labeling and molecular modeling studies also suggest the binding of Az to an ATPase domain, in particular, an ATP-binding pocket. It is proposed that Az suppresses ubiquitination of ΔF508-CFTR maybe by blocking interaction of the mutant with Hsc70 and CHIP, and, as a consequence, it enhances membrane trafficking of the mutant.-
dc.description.statementOfResponsibilityopen-
dc.format.extent20267~20276-
dc.relation.isPartOfJOURNAL OF THE AMERICAN CHEMICAL SOCIETY-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAdenosine Triphosphatases/chemistry-
dc.subject.MESHAdenosine Triphosphatases/metabolism*-
dc.subject.MESHBenzamides/chemistry-
dc.subject.MESHBenzamides/metabolism*-
dc.subject.MESHBinding Sites-
dc.subject.MESHCell Line-
dc.subject.MESHCystic Fibrosis Transmembrane Conductance Regulator/genetics-
dc.subject.MESHCystic Fibrosis Transmembrane Conductance Regulator/metabolism*-
dc.subject.MESHHSC70 Heat-Shock Proteins/metabolism*-
dc.subject.MESHHumans-
dc.subject.MESHImidazoles/chemistry-
dc.subject.MESHImidazoles/metabolism*-
dc.subject.MESHMagnetic Resonance Spectroscopy-
dc.subject.MESHMutation*-
dc.subject.MESHProtein Transport-
dc.subject.MESHSpectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization-
dc.subject.MESHUbiquitination-
dc.titleA small molecule that binds to an ATPase domain of Hsc70 promotes membrane trafficking of mutant cystic fibrosis transmembrane conductance regulator.-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Pharmacology (약리학)-
dc.contributor.googleauthorHyungseoph J. Cho-
dc.contributor.googleauthorHeon Yung Gee-
dc.contributor.googleauthorKyung-Hwa Baek-
dc.contributor.googleauthorSung-Kyun Ko-
dc.contributor.googleauthorJong-Moon Park-
dc.contributor.googleauthorHookeun Lee-
dc.contributor.googleauthorNam-Doo Kim-
dc.contributor.googleauthorMin Goo Lee-
dc.contributor.googleauthorInjae Shin-
dc.identifier.doi10.1021/ja206762p-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA02781-
dc.contributor.localIdA03971-
dc.relation.journalcodeJ01769-
dc.identifier.eissn1520-5126-
dc.identifier.pmid22074182-
dc.identifier.urlhttp://pubs.acs.org/doi/abs/10.1021/ja206762p-
dc.contributor.alternativeNameLee, Min Goo-
dc.contributor.affiliatedAuthorLee, Min Goo-
dc.contributor.affiliatedAuthorGee, Heon Yung-
dc.rights.accessRightsnot free-
dc.citation.volume133-
dc.citation.number50-
dc.citation.startPage20267-
dc.citation.endPage20276-
dc.identifier.bibliographicCitationJOURNAL OF THE AMERICAN CHEMICAL SOCIETY, Vol.133(50) : 20267-20276, 2011-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Pharmacology (약리학교실) > 1. Journal Papers

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.