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Genome-wide YFP fluorescence complementation screen identifies new regulators for telomere signaling in human cells
DC Field | Value | Language |
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dc.contributor.author | 이옥희 | - |
dc.date.accessioned | 2014-12-20T17:22:55Z | - |
dc.date.available | 2014-12-20T17:22:55Z | - |
dc.date.issued | 2011 | - |
dc.identifier.issn | 1535-9476 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/94495 | - |
dc.description.abstract | Detection of low-affinity or transient interactions can be a bottleneck in our understanding of signaling networks. To address this problem, we developed an arrayed screening strategy based on protein complementation to systematically investigate protein-protein interactions in live human cells, and performed a large-scale screen for regulators of telomeres. Maintenance of vertebrate telomeres requires the concerted action of members of the Telomere Interactome, built upon the six core telomeric proteins TRF1, TRF2, RAP1, TIN2, TPP1, and POT1. Of the ∼12,000 human proteins examined, we identified over 300 proteins that associated with the six core telomeric proteins. The majority of the identified proteins have not been previously linked to telomere biology, including regulators of post-translational modifications such as protein kinases and ubiquitin E3 ligases. Results from this study shed light on the molecular niche that is fundamental to telomere regulation in humans, and provide a valuable tool to investigate signaling pathways in mammalian cells. | - |
dc.description.statementOfResponsibility | open | - |
dc.format | application/pdf | - |
dc.relation.isPartOf | MOLECULAR & CELLULAR PROTEOMICS | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Bacterial Proteins/chemistry* | - |
dc.subject.MESH | Flow Cytometry/methods | - |
dc.subject.MESH | Genetic Complementation Test | - |
dc.subject.MESH | Genome | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Luminescent Proteins/chemistry* | - |
dc.subject.MESH | Protein Interaction Mapping | - |
dc.subject.MESH | Proteins/chemistry | - |
dc.subject.MESH | Proteome | - |
dc.subject.MESH | Retroviridae/genetics | - |
dc.subject.MESH | Signal Transduction | - |
dc.subject.MESH | Telomere/ultrastructure* | - |
dc.subject.MESH | Telomere-Binding Proteins/chemistry | - |
dc.subject.MESH | Ubiquitin/chemistry | - |
dc.subject.MESH | Ubiquitin-Protein Ligases/chemistry | - |
dc.title | Genome-wide YFP fluorescence complementation screen identifies new regulators for telomere signaling in human cells | - |
dc.type | Article | - |
dc.contributor.college | Researcher Institutes (부설 연구소) | - |
dc.contributor.department | Yonsei Integrative Research Institute for Cerebral & Cardiovascular Disease (뇌심혈관질환융합연구사업단) | - |
dc.contributor.googleauthor | Ok-Hee Lee | - |
dc.contributor.googleauthor | Hyeung Kim | - |
dc.contributor.googleauthor | Quanyuan He | - |
dc.contributor.googleauthor | Hwa Jin Baek | - |
dc.contributor.googleauthor | Dong Yang | - |
dc.contributor.googleauthor | Liuh-Yow Chen | - |
dc.contributor.googleauthor | Jiancong Liang | - |
dc.contributor.googleauthor | Heekyung Kate Chae | - |
dc.contributor.googleauthor | Amin Safari | - |
dc.contributor.googleauthor | Dan Liu | - |
dc.contributor.googleauthor | Zhou Songyang | - |
dc.identifier.doi | 10.1074/mcp.M110.001628–1 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A02970 | - |
dc.relation.journalcode | J02240 | - |
dc.identifier.eissn | 1535-9484 | - |
dc.identifier.pmid | 21044950 | - |
dc.contributor.alternativeName | Lee, Ok Hee | - |
dc.contributor.affiliatedAuthor | Lee, Ok Hee | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 10 | - |
dc.citation.number | 2 | - |
dc.citation.startPage | M110.001628 | - |
dc.identifier.bibliographicCitation | MOLECULAR & CELLULAR PROTEOMICS, Vol.10(2) : M110.001628, 2011 | - |
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