517 573

Cited 31 times in

A novel combination treatment of armed oncolytic adenovirus expressing IL-12 and GM-CSF with radiotherapy in murine hepatocarcinoma

DC Field Value Language
dc.contributor.author금웅섭-
dc.contributor.author김원우-
dc.contributor.author성진실-
dc.contributor.author오해진-
dc.contributor.author최경주-
dc.date.accessioned2014-12-20T17:18:37Z-
dc.date.available2014-12-20T17:18:37Z-
dc.date.issued2011-
dc.identifier.issn0449-3060-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/94357-
dc.description.abstractIn this study, a novel combination treatment of armed oncolytic adenovirus expressing interleukin 12 (IL-12) and granulocyte-macrophage colony-stimulating factor (GM-CSF) with radiation was investigated for antitumor and antimetastatic effect in a murine hepatic cancer (HCa-I) model. Tumor bearing syngeneic mice were treated with radiation, armed oncolytic virus Ad-ΔE1Bmt7 (dB7) expressing both IL-12 and GM-CSF (armed dB7), or a combination of both. The adenovirus was administered by intratumoral injection 1 × 10(8) PFU per tumor in 50 µl of PBS four times every other day. Tumor response to treatment was determined by a tumor growth delay assay. Metastatic potential was evaluated by a lung metastasis model. To understand the underlying mechanism, the level of apoptosis was examined as well as the change in microvessel density and expression of immunological markers: CD4+, CD8+ and Cd11c. The combination of armed dB7 and radiation resulted in significant growth delay of murine hepatic cancer, HCa-1, with an enhancement factor of 4.3. The combination treatment also resulted in significant suppression of lung metastasis. Increase of apoptosis level as well as decrease of microvessel density was shown in the combination treatment, suggesting an underlying mechanism for the enhancement of antitumor effect. Expression of immunological markers: CD4+, CD8+ and Cd11c also increased in the combination treatment. This study showed that a novel combination treatment of radiotherapy with armed oncolytic adenovirus expressing IL-12 and GM-CSF was effective in suppressing primary tumor growth.-
dc.description.statementOfResponsibilityopen-
dc.format.extent646~654-
dc.relation.isPartOfJOURNAL OF RADIATION RESEARCH-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAdenoviridae/genetics-
dc.subject.MESHAdenoviridae/pathogenicity-
dc.subject.MESHAnimals-
dc.subject.MESHApoptosis-
dc.subject.MESHCombined Modality Therapy-
dc.subject.MESHDendritic Cells/immunology-
dc.subject.MESHDendritic Cells/pathology-
dc.subject.MESHGranulocyte-Macrophage Colony-Stimulating Factor/genetics-
dc.subject.MESHInterleukin-12/genetics-
dc.subject.MESHLiver Neoplasms, Experimental/pathology-
dc.subject.MESHLiver Neoplasms, Experimental/radiotherapy*-
dc.subject.MESHLiver Neoplasms, Experimental/secondary-
dc.subject.MESHLiver Neoplasms, Experimental/therapy*-
dc.subject.MESHLung Neoplasms/secondary-
dc.subject.MESHLymphocytes, Tumor-Infiltrating/immunology-
dc.subject.MESHLymphocytes, Tumor-Infiltrating/pathology-
dc.subject.MESHMale-
dc.subject.MESHMice-
dc.subject.MESHMice, Inbred C3H-
dc.subject.MESHOncolyticVirotherapy*-
dc.subject.MESHOncolyticViruses/genetics-
dc.subject.MESHOncolyticViruses/pathogenicity-
dc.titleA novel combination treatment of armed oncolytic adenovirus expressing IL-12 and GM-CSF with radiotherapy in murine hepatocarcinoma-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Radiation Oncology (방사선종양학)-
dc.contributor.googleauthorWonwoo KIM-
dc.contributor.googleauthorJinsil SEONG-
dc.contributor.googleauthorHae Jin OH-
dc.contributor.googleauthorWoong Sub KOOM-
dc.contributor.googleauthorKyung-Joo CHOI-
dc.contributor.googleauthorChae-Ok YUN-
dc.identifier.doi10.1269/jrr.10185-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA00273-
dc.contributor.localIdA00768-
dc.contributor.localIdA01956-
dc.contributor.localIdA02413-
dc.contributor.localIdA04034-
dc.relation.journalcodeJ01727-
dc.identifier.eissn1349-9157-
dc.identifier.pmid21952320-
dc.subject.keywordOncolytic adenovirus-
dc.subject.keywordInterleukin 12-
dc.subject.keywordGranulocyte-macrophage colony-stimulating factor-
dc.subject.keywordRadiation-
dc.subject.keywordApoptosis-
dc.contributor.alternativeNameKoom, Woong Sub-
dc.contributor.alternativeNameKim, Won Woo-
dc.contributor.alternativeNameSeong, Jin Sil-
dc.contributor.alternativeNameOh, Hae Jin-
dc.contributor.alternativeNameChoi, Kyung Ju-
dc.contributor.affiliatedAuthorKoom, Woong Sub-
dc.contributor.affiliatedAuthorKim, Won Woo-
dc.contributor.affiliatedAuthorSeong, Jin Sil-
dc.contributor.affiliatedAuthorOh, Hae Jin-
dc.contributor.affiliatedAuthorChoi, Kyung Ju-
dc.rights.accessRightsfree-
dc.citation.volume52-
dc.citation.number5-
dc.citation.startPage646-
dc.citation.endPage654-
dc.identifier.bibliographicCitationJOURNAL OF RADIATION RESEARCH, Vol.52(5) : 646-654, 2011-
dc.identifier.rimsid27569-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Radiation Oncology (방사선종양학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers

qrcode

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.