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Gene expression profiling of human hepatoblastoma using archived formalin-fixed and paraffin-embedded tissues.

Authors
 Eun Shin  ;  Kyoung-Bun Lee  ;  Soo-Young Park  ;  Soo-Hee Kim  ;  Han-Suk Ryu  ;  Young-Nyun Park  ;  Eunsil Yu  ;  Ja-June Jang 
Citation
 VIRCHOWS ARCHIV, Vol.458(4) : 453-465, 2011 
Journal Title
VIRCHOWS ARCHIV
ISSN
 0945-6317 
Issue Date
2011
MeSH
Adolescent ; Biomarkers, Tumor/analysis* ; Biomarkers, Tumor/genetics ; Child ; Child, Preschool ; Female ; Formaldehyde ; Gene Expression ; Gene Expression Profiling/methods* ; Hepatoblastoma/genetics* ; Hepatoblastoma/mortality ; Hepatoblastoma/pathology ; Humans ; Immunohistochemistry ; Infant ; Kaplan-Meier Estimate ; Liver Neoplasms/genetics* ; Liver Neoplasms/mortality ; Liver Neoplasms/pathology ; Male ; Oligonucleotide Array Sequence Analysis ; Paraffin Embedding ; Prognosis ; RNA, Messenger/analysis ; RNA, Messenger/isolation & purification ; Reverse Transcriptase Polymerase Chain Reaction ; Tissue Fixation
Keywords
Hepatoblastoma ; DASL chip assay ; YY1 ; Prognosis
Abstract
We elucidated the genetic profile of hepatoblastomas (HBLs) to identify diagnostic and prognostic markers. RNA was extracted from 32 formalin-fixed, paraffin-embedded HBLs and corresponding nonneoplastic liver (NNL) tissues, and cDNA-mediated annealing, selection, extension, and ligation (DASL) chip assays were performed. Immunohistochemistry was performed to confirm the expression of Yin Yang 1 (YY1) protein in HBL. Twenty-four genes that were associated with signal transduction, cell-cell adhesion, cell cycle regulation, and apoptosis were differentially expressed in HBL and NNL tissues. Two apoptosis-associated genes, MYCN and BIRC5, were highly upregulated in HBL. Eight genes, including YY1 and IGF1, were upregulated in HBL cases that had a poor prognosis. Thirty-eight genes, including YY1, were differentially expressed according to histologic differentiation of HBL, and the immunohistochemical expression of YY1 was correlated with poor HBL differentiation. Thus, using DASL chip assays, we report the gene expression profiles of HBL, which suggest new candidate prognostic and diagnostic genetic markers and putative therapeutic targets for HBL.
Full Text
http://link.springer.com/article/10.1007%2Fs00428-011-1043-8
DOI
10.1007/s00428-011-1043-8
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers
Yonsei Authors
Park, Young Nyun(박영년) ORCID logo https://orcid.org/0000-0003-0357-7967
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/94194
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