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Gene expression profiling of human hepatoblastoma using archived formalin-fixed and paraffin-embedded tissues.

Authors
 Kyoung-Bun Lee ; Eun Shin ; Ja-June Jang ; Eunsil Yu ; Young-Nyun Park ; Han-Suk Ryu ; Soo-Hee Kim ; Soo-Young Park 
Citation
 Virchows Archiv, Vol.458(4) : 453~465, 2011 
Journal Title
 Virchows Archiv 
ISSN
 0945-6317 
Issue Date
2011
Abstract
We elucidated the genetic profile of hepatoblastomas (HBLs) to identify diagnostic and prognostic markers. RNA was extracted from 32 formalin-fixed, paraffin-embedded HBLs and corresponding nonneoplastic liver (NNL) tissues, and cDNA-mediated annealing, selection, extension, and ligation (DASL) chip assays were performed. Immunohistochemistry was performed to confirm the expression of Yin Yang 1 (YY1) protein in HBL. Twenty-four genes that were associated with signal transduction, cell-cell adhesion, cell cycle regulation, and apoptosis were differentially expressed in HBL and NNL tissues. Two apoptosis-associated genes, MYCN and BIRC5, were highly upregulated in HBL. Eight genes, including YY1 and IGF1, were upregulated in HBL cases that had a poor prognosis. Thirty-eight genes, including YY1, were differentially expressed according to histologic differentiation of HBL, and the immunohistochemical expression of YY1 was correlated with poor HBL differentiation. Thus, using DASL chip assays, we report the gene expression profiles of HBL, which suggest new candidate prognostic and diagnostic genetic markers and putative therapeutic targets for HBL.
URI
http://ir.ymlib.yonsei.ac.kr/handle/22282913/94194
DOI
10.1007/s00428-011-1043-8
Appears in Collections:
1. 연구논문 > 1. College of Medicine > Dept. of Pathology
Yonsei Authors
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Link
 http://link.springer.com/article/10.1007%2Fs00428-011-1043-8
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