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NOX1 participates in ROS-dependent cell death of colon epithelial Caco2 cells induced by Entamoeba histolytica

Authors
 Kyeong Ah Kim  ;  Ju Young Kim  ;  Young Ah Lee  ;  Kyoung-Ju Song  ;  Deulle Min  ;  Myeong Heon Shin 
Citation
 MICROBES AND INFECTION, Vol.13(12-13) : 1052-1061, 2011 
Journal Title
MICROBES AND INFECTION
ISSN
 1286-4579 
Issue Date
2011
MeSH
Animals ; Caco-2Cells ; CellDeath ; Entamoebahistolytica/cytology ; Entamoebahistolytica/physiology* ; Entamoebiasis/microbiology* ; Enzyme Inhibitors/pharmacology* ; Humans ; NADPH Oxidase 1 ; NADPH Oxidases/antagonists & inhibitors ; NADPH Oxidases/genetics ; NADPH Oxidases/immunology ; NADPH Oxidases/metabolism* ; Onium Compounds/pharmacology* ; RNA, Small Interfering/physiology ; Rabbits ; Reactive Oxygen Species/metabolism* ; Signal Transduction
Keywords
Entamoeba histolytica ; Cell death ; Colon epithelial Caco2 cells ; ROS ; NOX1 (NADPH oxidase 1)
Abstract
Entamoeba histolytica, which causes amebic colitis and occasional liver abscesses in humans, can induce host cell death through apoptosis and necrosis. Recently, we have demonstrated that E. histolytica can induce cell death in neutrophils via diphenyleneiodonium-sensitive NADPH oxidase (NOX)-derived reactive oxygen species (ROS). Although there are enzyme systems similar to the phagocyte NADPH oxidase system in many non-phagocytic cell types, the signaling role of NOX-derived ROS in cell death of human colon epithelial cells induced by E. histolytica remains obscure. Incubation of colon epithelial Caco2 tumor cell lines with amebic trophozoites resulted in intracellular ROS generation and cell death in a caspase-independent manner. Pretreatment with DPI, an inhibitor of NOX, strongly decreased E. histolytica-induced cell death in Caco2 cells. As identified by RT-PCR, NOX1 transcripts were highly expressed in Caco2 cells. siRNA-mediated suppression of NOX1 protein significantly inhibited E. histolytica-induced cell death and ROS response in Caco2 cells. These results suggest that NOX1 participates in the ROS-dependent cell death of colon epithelial cells induced by amebic adhesion during the early phase of intestinal amebiasis.
Full Text
http://www.sciencedirect.com/science/article/pii/S1286457911001456
DOI
10.1016/j.micinf.2011.06.001
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Tropica Medicine (열대의학교실) > 1. Journal Papers
Yonsei Authors
Kim, Kyung Ah(김경아)
Kim, Ju Yeong(김주영) ORCID logo https://orcid.org/0000-0003-2456-6298
Min, A Rim(민아림) ORCID logo https://orcid.org/0000-0003-2938-9630
Song, Kyoung Ju(송경주)
Shin, Myeong Heon(신명헌) ORCID logo https://orcid.org/0000-0001-8207-6110
Lee, Young Ah(이영아) ORCID logo https://orcid.org/0000-0002-0414-842X
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/94125
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