Cited 30 times in
Apoptosis signal-regulating kinase 1 (Ask1) targeted small interfering RNA on ischemic neuronal cell death
DC Field | Value | Language |
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dc.contributor.author | 김경환 | - |
dc.contributor.author | 김현우 | - |
dc.contributor.author | 이수경 | - |
dc.contributor.author | 조경주 | - |
dc.date.accessioned | 2014-12-20T16:57:49Z | - |
dc.date.available | 2014-12-20T16:57:49Z | - |
dc.date.issued | 2011 | - |
dc.identifier.issn | 0006-8993 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/93710 | - |
dc.description.abstract | Apoptosis signal-regulating kinase 1 (Ask1) is one of mitogen-activated protein kinase kinase kinase (MAPKKK) for cell differentiation and apoptosis. The aim of the present study is to evaluate whether RNA interference against Ask1 (Ask1-siRNA) down-regulates the expression of Ask1 and prevents apoptotic neuronal cell death after ischemia/reperfusion (I/R) in mice. Mice were subjected to intraluminal suture occlusion of the middle cerebral artery for 1h, followed by reperfusion. The Ask1-siRNA or a control-siRNA was introduced using osmotic pump intracerebroventricularly at 3days before I/R. The expression and mRNA of Ask1 were evaluated by Western blot and RT-PCR after I/R with time. Immunohistochemistry and TUNEL assay were also investigated to evaluate the effect of Ask1 on cerebral infarction by Ask1-siRNA treatment. The expression of Ask1 was increased significantly at 8h after I/R. The level of mRNA and protein of Ask1 down-regulated after treatment of Ask1-siRNA and subsequently cerebral infarction volume was reduced. Our results suggest the increased Ask1 expression induce apoptotic cell death after I/R. And we also demonstrated that Ask1-siRNA attenuates upregulation of Ask1, which was followed by the reduction of infarction in ischemic brain after I/R. Ask1-siRNA could represent a molecular target for prevention of ischemic stroke. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 73~78 | - |
dc.relation.isPartOf | BRAIN RESEARCH | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Brain/metabolism | - |
dc.subject.MESH | Brain/pathology* | - |
dc.subject.MESH | Brain Ischemia/genetics* | - |
dc.subject.MESH | Brain Ischemia/metabolism | - |
dc.subject.MESH | Brain Ischemia/pathology | - |
dc.subject.MESH | Cell Death | - |
dc.subject.MESH | MAP Kinase Kinase Kinase 5/genetics* | - |
dc.subject.MESH | MAP Kinase Kinase Kinase 5/metabolism | - |
dc.subject.MESH | Male | - |
dc.subject.MESH | Mice | - |
dc.subject.MESH | Neurons/metabolism | - |
dc.subject.MESH | Neurons/pathology* | - |
dc.subject.MESH | Phosphorylation | - |
dc.subject.MESH | RNA, Small Interfering | - |
dc.subject.MESH | Reperfusion | - |
dc.subject.MESH | Stroke/genetics* | - |
dc.subject.MESH | Stroke/metabolism | - |
dc.subject.MESH | Stroke/pathology | - |
dc.title | Apoptosis signal-regulating kinase 1 (Ask1) targeted small interfering RNA on ischemic neuronal cell death | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Yonsei Biomedical Research Center (연세의생명연구원) | - |
dc.contributor.googleauthor | Hyun-Woo Kim | - |
dc.contributor.googleauthor | Kyoung-Joo Cho | - |
dc.contributor.googleauthor | Su Kyoung Lee | - |
dc.contributor.googleauthor | Gyung W. Kim | - |
dc.identifier.doi | 10.1016/j.brainres.2011.07.018 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A00310 | - |
dc.contributor.localId | A02888 | - |
dc.contributor.localId | A03804 | - |
dc.contributor.localId | A01125 | - |
dc.relation.journalcode | J00392 | - |
dc.identifier.eissn | 1872-6240 | - |
dc.identifier.pmid | 21803338 | - |
dc.identifier.url | http://www.sciencedirect.com/science/article/pii/S0006899311012765 | - |
dc.subject.keyword | Stroke | - |
dc.subject.keyword | Cerebral infarction | - |
dc.subject.keyword | Apoptosis signal-regulating kinase 1 | - |
dc.subject.keyword | RNA interference | - |
dc.subject.keyword | Mice | - |
dc.contributor.alternativeName | Kim, Gyung Whan | - |
dc.contributor.alternativeName | Kim, Hyun Woo | - |
dc.contributor.alternativeName | Lee, Su Kyoung | - |
dc.contributor.alternativeName | Cho, Kyuong Joo | - |
dc.contributor.affiliatedAuthor | Kim, Gyung Whan | - |
dc.contributor.affiliatedAuthor | Lee, Su Kyoung | - |
dc.contributor.affiliatedAuthor | Cho, Kyuong Joo | - |
dc.contributor.affiliatedAuthor | Kim, Hyun Woo | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 1412 | - |
dc.citation.startPage | 73 | - |
dc.citation.endPage | 78 | - |
dc.identifier.bibliographicCitation | BRAIN RESEARCH, Vol.1412 : 73-78, 2011 | - |
dc.identifier.rimsid | 28410 | - |
dc.type.rims | ART | - |
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