Cited 21 times in
Carmustine induces ERK- and JNK-dependent cell death of neuronally-differentiated PC12 cells via generation of reactive oxygen species
DC Field | Value | Language |
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dc.contributor.author | 서정택 | - |
dc.contributor.author | 신동민 | - |
dc.contributor.author | 안정미 | - |
dc.date.accessioned | 2014-12-20T16:55:44Z | - |
dc.date.available | 2014-12-20T16:55:44Z | - |
dc.date.issued | 2011 | - |
dc.identifier.issn | 0887-2333 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/93646 | - |
dc.description.abstract | Accumulation of reactive oxygen species (ROS) caused by the inhibition of glutathione reductase (GR) has been proposed as one of the mechanisms responsible for carmustine (1,3-bis(2-chloroethyl)-1-nitrosourea, BCNU)-induced cytotoxicity. Since mitogen-activated protein kinases (MAPKs) are known to mediate ROS-dependent cell death in multiple cell types, we examined whether redox-sensitive MAPK activation mediated the carmustine-induced cell death of neuronally differentiated PC12 cells. Carmustine induced a concentration- and time-dependent cell death, which was associated with increased caspase-3 activation, a reduction in GR activity accompanied by a concomitant decrease in reduced glutathione levels, and accumulation of ROS. Carmustine-induced caspase-3 activation and cell death were prevented by pretreatment with anti-oxidants or a reducing agent, indicating that carmustine-induced caspase-3 activation and cell death occur via redox-dependent processes. Carmustine induced phosphorylation of the MAPKs, such as extracellular signal-regulated kinase (ERK), c-Jun N-terminal kinase (JNK), and p38. The activation of these kinases was inhibited by pretreatment with N-acetyl-L-cysteine (NAC). Although all the MAPKs were activated by carmustine, only the inhibitors of JNK and ERK prevented carmustine-induced cell death and caspase-3 activation. Our data suggest that carmustine-induced neurotoxicity is, at least in part, due to the activation of ROS-dependent JNK and ERK signaling. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 1359~1365 | - |
dc.relation.isPartOf | TOXICOLOGY IN VITRO | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Acetylcysteine/pharmacology | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Antineoplastic Agents, Alkylating/pharmacology | - |
dc.subject.MESH | Carmustine/pharmacology* | - |
dc.subject.MESH | Caspase 3/metabolism | - |
dc.subject.MESH | Cell Death/drug effects | - |
dc.subject.MESH | Enzyme Activation | - |
dc.subject.MESH | Extracellular Signal-Regulated MAP Kinases/antagonists & inhibitors | - |
dc.subject.MESH | Extracellular Signal-Regulated MAP Kinases/genetics | - |
dc.subject.MESH | Extracellular Signal-Regulated MAP Kinases/metabolism* | - |
dc.subject.MESH | Glutathione Reductase/antagonists & inhibitors | - |
dc.subject.MESH | MAP Kinase Kinase 4/antagonists & inhibitors | - |
dc.subject.MESH | MAP Kinase Kinase 4/genetics | - |
dc.subject.MESH | MAP Kinase Kinase 4/metabolism* | - |
dc.subject.MESH | Neurons/cytology | - |
dc.subject.MESH | Neurons/drug effects* | - |
dc.subject.MESH | PC12 Cells | - |
dc.subject.MESH | Phosphorylation | - |
dc.subject.MESH | Rats | - |
dc.subject.MESH | Reactive Oxygen Species/metabolism* | - |
dc.subject.MESH | p38 Mitogen-Activated Protein Kinases/genetics | - |
dc.subject.MESH | p38 Mitogen-Activated Protein Kinases/metabolism | - |
dc.title | Carmustine induces ERK- and JNK-dependent cell death of neuronally-differentiated PC12 cells via generation of reactive oxygen species | - |
dc.type | Article | - |
dc.contributor.college | College of Dentistry (치과대학) | - |
dc.contributor.department | Dept. of Oral Biology (구강생물학) | - |
dc.contributor.googleauthor | Jeong Mi An | - |
dc.contributor.googleauthor | Seon Sook Kim | - |
dc.contributor.googleauthor | Jin Hak Rhie | - |
dc.contributor.googleauthor | Dong Min Shin | - |
dc.contributor.googleauthor | Su Ryeon Seo | - |
dc.contributor.googleauthor | Jeong Taeg Seo | - |
dc.identifier.doi | 10.1016/j.tiv.2011.05.006 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A01905 | - |
dc.contributor.localId | A02091 | - |
dc.contributor.localId | A02257 | - |
dc.relation.journalcode | J02743 | - |
dc.identifier.eissn | 1879-3177 | - |
dc.identifier.pmid | 21600974 | - |
dc.identifier.url | http://www.sciencedirect.com/science/article/pii/S0887233311001317 | - |
dc.subject.keyword | Carmustine | - |
dc.subject.keyword | Reactive oxygen species | - |
dc.subject.keyword | ERK | - |
dc.subject.keyword | JNK | - |
dc.contributor.alternativeName | Seo, Jeong Taeg | - |
dc.contributor.alternativeName | Shin, Dong Min | - |
dc.contributor.alternativeName | An, Jeong Mi | - |
dc.contributor.affiliatedAuthor | Seo, Jeong Taeg | - |
dc.contributor.affiliatedAuthor | Shin, Dong Min | - |
dc.contributor.affiliatedAuthor | An, Jeong Mi | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 25 | - |
dc.citation.number | 7 | - |
dc.citation.startPage | 1359 | - |
dc.citation.endPage | 1365 | - |
dc.identifier.bibliographicCitation | TOXICOLOGY IN VITRO, Vol.25(7) : 1359-1365, 2011 | - |
dc.identifier.rimsid | 28364 | - |
dc.type.rims | ART | - |
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