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Emergence of Clostridium difficile ribotype 027 in Korea.

Authors
 Heejung Kim, M.D.  ;  Yangsoon Lee, M.D.  ;  Hee-Won Moon, M.D.  ;  Chae Seung Lim, M.D.  ;  Kyungwon Lee, M.D.  ;  Yunsop Chong, M.D. 
Citation
 KOREAN JOURNAL OF LABORATORY MEDICINE , Vol.31(3) : 191-196, 2011 
Journal Title
 KOREAN JOURNAL OF LABORATORY MEDICINE 
ISSN
 1598-6535 
Issue Date
2011
MeSH
Adult ; Aged ; Aged, 80 and over ; Bacterial Proteins/genetics ; Bacterial Proteins/metabolism ; Bacterial Toxins/genetics ; Bacterial Toxins/metabolism ; Clostridium difficile/genetics ; Clostridium difficile/isolation & purification* ; Drug Resistance, Bacterial ; Electrophoresis, Gel, Pulsed-Field ; Enterocolitis, Pseudomembranous/microbiology ; Enterotoxins/genetics ; Enterotoxins/metabolism ; Feces/microbiology ; Female ; Humans ; Male ; Microbial Sensitivity Tests ; Middle Aged ; Mutation ; Polymerase Chain Reaction ; Republic of Korea ; Ribotyping*
Keywords
Clostridium difficile ; Multilocus variable-number tandem-repeat analysis ; PCR-ribotype 027 ; Pulsed-field gel electrophoresis
Abstract
BACKGROUND: Clostridium difficile infection (CDI) has markedly risen and is associated with hypervirulent ribotype 027 outbreaks in North America and Europe since 2003. The aims of this study were to determine the prevalence of ribotype 027 among C. difficile isolates in Korea, to characterize the ribotype 027 isolates, and to determine the clinical severity of CDI in patients infected with these isolates. METHODS: A total of 1,251 isolates of C. difficile recovered from stool specimens of suspected CDI patients at two tertiary-care hospitals and one commercial laboratory between 2002 and 2009. Genes for toxin A (tcdA), toxin B (tcdB), and binary toxin (cdtA and cdtB) were detected by PCR. Mutation in the tcdC gene was detected by sequencing after PCR amplification. For molecular genotyping, we performed PCR-ribotyping, pulsed-field gel electrophoresis (PFGE), and multilocus variable-number tandem-repeat analysis (MLVA). Minimum inhibitory concentrations of moxifloxacin were determined using Etest strips (AB bioMérieux, Sweden). RESULTS: We identified 7 isolates as ribotype 027. These isolates had the same tcdC mutation as the epidemic strain, and 6 of them were resistant to moxifloxacin. The isolates were categorized into 3 different PFGE types and 7 different MLVA types. All the 7 cases had occurred sporadically. CONCLUSIONS: C. difficile ribotype 027 is uncommon, but it has emerged in Korea. The spread of this ribotype should be closely monitored in order to avoid an outbreak of CDI in Korea.
Files in This Item:
T201102702.pdf Download
DOI
10.3343/kjlm.2011.31.3.191
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
Yonsei Authors
Kim, Heejung(김희정) ORCID logo https://orcid.org/0000-0002-0190-703X
Lee, Kyungwon(이경원) ORCID logo https://orcid.org/0000-0003-3788-2134
Lee, Yang Soon(이양순)
Chong, Yun Sop(정윤섭)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/93624
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