Cited 69 times in
Identification of lipopolysaccharide-binding peptide regions within HMGB1 and their effects on subclinical endotoxemia in a mouse model.
DC Field | Value | Language |
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dc.contributor.author | 곽만섭 | - |
dc.contributor.author | 신전수 | - |
dc.contributor.author | 윤주호 | - |
dc.date.accessioned | 2014-12-20T16:54:47Z | - |
dc.date.available | 2014-12-20T16:54:47Z | - |
dc.date.issued | 2011 | - |
dc.identifier.issn | 0014-2980 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/93615 | - |
dc.description.abstract | Lipopolysaccharide (LPS) triggers deleterious systemic inflammatory responses when released into the circulation. LPS-binding protein (LBP) in the serum plays an important role in modifying LPS toxicity by facilitating its interaction with LPS signaling receptors, which are expressed on the surface of LPS-responsive cells. We have previously demonstrated that high mobility group box 1 (HMGB1) can bind to and transfer LPS, consequently increasing LPS-induced TNF-α production in human peripheral blood mononuclear cells (PBMCs). We report here on the identification of two LPS-binding domains within HMGB1. Furthermore, using 12 synthetic HMGB1 peptides, we define the LPS-binding regions within each domain. Among them, synthetic peptides HPep1 and HPep6, which are located in the A and B box domains of HMGB1, bind to the polysaccharide and lipid A moieties of LPS respectively. Both HPep1 and HPep6 peptides inhibited binding of LPS to LBP and HMGB1, LBP-mediated LPS transfer to CD14, and cellular uptake of LPS in RAW264.7 cells. These peptides also inhibited LPS-induced TNF-α release in human PBMCs and induced lower levels of TNF-α in the serum in a subclinical endotoxemia mouse model. These results indicate that HMGB1 has two LPS-binding peptide regions that can be utilized to design anti-sepsis or LPS-neutralizing therapeutics | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 2753~2762 | - |
dc.relation.isPartOf | EUROPEAN JOURNAL OF IMMUNOLOGY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Acute-Phase Proteins/antagonists & inhibitors | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Binding Sites/drug effects | - |
dc.subject.MESH | Carrier Proteins/antagonists & inhibitors | - |
dc.subject.MESH | Cell Line | - |
dc.subject.MESH | Disease Models, Animal | - |
dc.subject.MESH | Endotoxemia/blood | - |
dc.subject.MESH | Endotoxemia/immunology* | - |
dc.subject.MESH | HMGB1 Protein/chemistry | - |
dc.subject.MESH | HMGB1 Protein/immunology | - |
dc.subject.MESH | HMGB1 Protein/metabolism* | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Leukocytes, Mononuclear/drug effects | - |
dc.subject.MESH | Leukocytes, Mononuclear/immunology | - |
dc.subject.MESH | Leukocytes, Mononuclear/metabolism* | - |
dc.subject.MESH | Leukocytes, Mononuclear/pathology | - |
dc.subject.MESH | Lipopolysaccharides/immunology | - |
dc.subject.MESH | Lipopolysaccharides/metabolism* | - |
dc.subject.MESH | Membrane Glycoproteins/antagonists & inhibitors | - |
dc.subject.MESH | Mice | - |
dc.subject.MESH | Mice, Inbred BALB C | - |
dc.subject.MESH | Peptide Fragments/administration & dosage | - |
dc.subject.MESH | Peptide Fragments/chemical synthesis | - |
dc.subject.MESH | Peptide Fragments/metabolism* | - |
dc.subject.MESH | Protein Binding/drug effects | - |
dc.subject.MESH | Tumor Necrosis Factor-alpha/blood | - |
dc.title | Identification of lipopolysaccharide-binding peptide regions within HMGB1 and their effects on subclinical endotoxemia in a mouse model. | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Microbiology (미생물학) | - |
dc.contributor.googleauthor | Ju Ho Youn | - |
dc.contributor.googleauthor | Man Sup Kwak | - |
dc.contributor.googleauthor | Jie Wu | - |
dc.contributor.googleauthor | Eun Sook Kim | - |
dc.contributor.googleauthor | Yeounjung Ji | - |
dc.contributor.googleauthor | Hyun Jin Min | - |
dc.contributor.googleauthor | Ji-Ho Yoo | - |
dc.contributor.googleauthor | Ji Eun Choi | - |
dc.contributor.googleauthor | Hyun-Soo Cho | - |
dc.contributor.googleauthor | Jeon-Soo Shin | - |
dc.identifier.doi | 10.1002/eji.201141391 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A00166 | - |
dc.contributor.localId | A02144 | - |
dc.contributor.localId | A02605 | - |
dc.relation.journalcode | J00825 | - |
dc.identifier.eissn | 1521-4141 | - |
dc.identifier.pmid | 21660935 | - |
dc.subject.keyword | Endotoxin shock | - |
dc.subject.keyword | High mobility group box 1 | - |
dc.subject.keyword | Inflammation | - |
dc.subject.keyword | Lipopoly saccharide | - |
dc.contributor.alternativeName | Kwak, Man Sup | - |
dc.contributor.alternativeName | Shin, Jeon Soo | - |
dc.contributor.alternativeName | Youn, Ju Ho | - |
dc.contributor.affiliatedAuthor | Kwak, Man Sup | - |
dc.contributor.affiliatedAuthor | Shin, Jeon Soo | - |
dc.contributor.affiliatedAuthor | Youn, Ju Ho | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 41 | - |
dc.citation.number | 9 | - |
dc.citation.startPage | 2753 | - |
dc.citation.endPage | 2762 | - |
dc.identifier.bibliographicCitation | EUROPEAN JOURNAL OF IMMUNOLOGY, Vol.41(9) : 2753-2762, 2011 | - |
dc.identifier.rimsid | 28342 | - |
dc.type.rims | ART | - |
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