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In vitro activity of xanthorrhizol against Candida glabrata, C. guilliermondii, and C. parapsilosis biofilms

Authors
 Yaya Rukayadi ; Sunghwa Han ; Jae-Kwan Hwang ; Dongeun Yong 
Citation
 Medical Mycology, Vol.49(1) : 1~9, 2011 
Journal Title
 Medical Mycology 
ISSN
 1369-3786 
Issue Date
2011
Abstract
The formation of Candida biofilms has important clinical ramifications, because these biofilms exhibit increased resistance to conventional antifungal therapies. The aim of this study was to investigate the activity of xanthorrhizol on biofilms produced by non-C. albicans Candida (NCAC) species, including C. glabrata, C. guilliermondii, and C. parapsilosis. NCAC biofilms were generated in flat-bottom 96-well microtiter plates and quantified using the XTT (2, 3 - bis (2-methoxy-4-nitro-5-sulfophenyl)-5-[(phenyl amino) carbonyl]-2H-tetrazolium hydroxide) reduction assay. The NCAC biofilms at adherent, intermediate, and mature growth phases were treated with 0.5-512 μg/ml of xanthorrhizol for 24 h. The ranges of sessile minimum inhibitory concentrations (SMICs) of xanthorrhizol against C. glabrata, C. guilliermondii, and C. parapsilosis biofilms were 8-32 μg/ml, 8-16 μg/ml, and 8-64 μg/ml, respectively. Xanthorrhizol affected cell density that had an indirect effect on the biofilm OD(490). The compound eradicated the viable cells of the C. glabrata and C. parapsilosis biofilms at the adherent growth phase at 16 μg/ml and that of C. guilliermondii at 8 μg/ml. Treatment with 128 μg/ml of xanthorrhizol reduced the OD(490) of C. glabrata, C. guilliermondii, and C. parapsilosis biofilms at the mature growth phase by 77.8%, 88.5%, and 64.5%, respectively. These results indicate that xanthorrhizol exhibits potent activity against NCAC biofilms in vitro. Therefore, xanthorrhizol has potential therapeutic value in treating biofilm-associated NCAC infections and should be further evaluated in vivo.
URI
http://ir.ymlib.yonsei.ac.kr/handle/22282913/93372
DOI
10.3109/13693786.2010.492482
Appears in Collections:
1. 연구논문 > 1. College of Medicine > Dept. of Laboratory Medicine
Yonsei Authors
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Link
 http://informahealthcare.com/doi/abs/10.3109/13693786.2010.492482
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