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Diphenyleneiodonium inhibits the activation of mitogen-activated protein kinases and the expression of monocyte chemoattractant protein-1 in Helicobacter pylori-infected gastric epithelial AGS cells.
DC Field | Value | Language |
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dc.contributor.author | 김경환 | - |
dc.date.accessioned | 2014-12-20T16:47:03Z | - |
dc.date.available | 2014-12-20T16:47:03Z | - |
dc.date.issued | 2011 | - |
dc.identifier.issn | 1023-3830 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/93370 | - |
dc.description.abstract | OBJECTIVE: To investigate whether NADPH oxidase induces MCP-1 expression and the activation of mitogen-activated protein kinases (MAPKs) in H. pylori-infected gastric epithelial cells. MATERIAL: H. pylori in Korean isolates, human gastric epithelial AGS cells TREATMENT: AGS cells pretreated with or without an NADPH oxidase inhibitor diphenyleneiodonium (DPI) are cultured in the presence of H. pylori at a bacterium/cell ratio of 300:1. METHODS: Reactive oxygen species (ROS) and MCP-1 were determined by confocal microscopy and enzyme-linked immonosorbent assay. NADPH oxidase activity was measured by lucigenin assay. mRNA expression of MCP-1 was analyzed by reverse transcription-polymerase chain reaction. Levels of MAPKs were assessed by Western blot analysis. RESULTS: H. pylori induced increase in ROS, NADPH oxidase activity, MCP-1 expression, and the activation of MAPKs including extracellular signal-regulated kinases, p38, and jun N-terminal kinases in AGS cells, which was inhibited by DPI. CONCLUSION: Inhibiting NADPH oxidase by DPI suppresses H. pylori-induced activation of MAPKs and MCP-1 expression in AGS cells | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | 501~507 | - |
dc.relation.isPartOf | INFLAMMATION RESEARCH | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Cell Line | - |
dc.subject.MESH | Chemokine CCL2/metabolism* | - |
dc.subject.MESH | Cytokines/metabolism | - |
dc.subject.MESH | Dose-Response Relationship, Drug | - |
dc.subject.MESH | Enzyme Activation | - |
dc.subject.MESH | Epithelial Cells/cytology* | - |
dc.subject.MESH | Helicobacter pylori/metabolism* | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Inflammation | - |
dc.subject.MESH | Korea | - |
dc.subject.MESH | MAP Kinase Signaling System* | - |
dc.subject.MESH | NADPH Oxidases/antagonists & inhibitors | - |
dc.subject.MESH | NADPH Oxidases/metabolism | - |
dc.subject.MESH | Onium Compounds/pharmacology | - |
dc.subject.MESH | Reactive Oxygen Species | - |
dc.subject.MESH | Stomach/metabolism* | - |
dc.title | Diphenyleneiodonium inhibits the activation of mitogen-activated protein kinases and the expression of monocyte chemoattractant protein-1 in Helicobacter pylori-infected gastric epithelial AGS cells. | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Pharmacology (약리학) | - |
dc.contributor.googleauthor | Soon Ok Cho | - |
dc.contributor.googleauthor | Joo Weon Lim | - |
dc.contributor.googleauthor | Kyung Hwan Kim | - |
dc.contributor.googleauthor | Hyeyoung Kim | - |
dc.identifier.doi | 10.1007/s00011-010-0297-y | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A00311 | - |
dc.relation.journalcode | J01059 | - |
dc.identifier.eissn | 1420-908X | - |
dc.identifier.pmid | 21181544 | - |
dc.identifier.url | http://link.springer.com/article/10.1007%2Fs00011-010-0297-y | - |
dc.subject.keyword | Helicobacter pylori | - |
dc.subject.keyword | NADPH oxidase | - |
dc.subject.keyword | Monocyte chemoattractant protein-1 | - |
dc.subject.keyword | Mitogen-activated protein kinase | - |
dc.subject.keyword | Gastric epithelial cells | - |
dc.contributor.alternativeName | Kim, Kyung Hwan | - |
dc.contributor.affiliatedAuthor | Kim, Kyung Hwan | - |
dc.rights.accessRights | not free | - |
dc.citation.volume | 60 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 501 | - |
dc.citation.endPage | 507 | - |
dc.identifier.bibliographicCitation | INFLAMMATION RESEARCH, Vol.60(5) : 501-507, 2011 | - |
dc.identifier.rimsid | 27168 | - |
dc.type.rims | ART | - |
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