3 521

Cited 5 times in

A new versatile peptide-based size exclusion chromatography platform for global profiling and quantitation of candidate biomarkers in hepatocellular carcinoma specimens

 Hyoung-Joo Lee  ;  Keun Na  ;  Min-Seok Kwon  ;  Taesung Park  ;  Kyung Sik Kim  ;  Hoguen Kim  ;  Young-Ki Paik 
 PROTEOMICS, Vol.11(10) : 1976-1984, 2011 
Journal Title
Issue Date
Amino Acid Sequence ; Biomarkers, Tumor/analysis* ; Biomarkers, Tumor/blood ; Carcinoma, Hepatocellular/blood ; Carcinoma, Hepatocellular/chemistry* ; Carcinoma, Hepatocellular/metabolism ; Chromatography, Gel/methods* ; Chromatography, Liquid ; Glycopeptides/analysis ; Humans ; Liver/chemistry ; Liver Neoplasms/blood ; Liver Neoplasms/chemistry* ; Liver Neoplasms/metabolism ; Molecular Sequence Data ; Neoplasm Proteins/analysis ; Neoplasm Proteins/metabolism ; Peptide Fragments/analysis* ; Peptide Fragments/metabolism ; Peptide Mapping ; Proteomics ; Tandem Mass Spectrometry
Disease biomarkers are predicted to be in low abundance; thus, the most crucial step of biomarker discovery is the efficient fractionation of clinical samples into protein sets that define disease stages and/or predict disease development. For this purpose, we developed a new platform that uses peptide-based size exclusion chromatography (pep-SEC) to quantify disease biomarker candidates. This new platform has many advantages over previously described biomarker profiling platforms, including short run time, high resolution, and good reproducibility, which make it suitable for large-scale analysis. We combined this platform with isotope labeling and label-free methods to identify and quantitate differentially expressed proteins in hepatocellular carcinoma (HCC) tissues. When we combined pep-SEC with a gas phase fractionation method, which broadens precursor ion selection, the protein coverage was significantly increased, which is critical for the global profiling of HCC specimens. Furthermore, pep-SEC-LC-MS/MS analysis enhanced the detection of low-abundance proteins (e.g. insulin receptor substrate 2 and carboxylesterase 1) and glycopeptides in HCC plasma. Thus, our pep-SEC platform is an efficient and versatile pre-fractionation system for the large-scale profiling and quantitation of candidate biomarkers in complex disease proteomes.
Full Text
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Surgery (외과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Kyung Sik(김경식) ORCID logo https://orcid.org/0000-0001-9498-284X
Kim, Hogeun(김호근)
사서에게 알리기


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.