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TTF-1 mRNA-positive circulating tumor cells in the peripheral blood predict poor prognosis in surgically resected non-small cell lung cancer patients.

Authors
 Sun Och Yoon  ;  Young Tae Kim  ;  Kyeong Cheon Jung  ;  Yoon Kyung Jeon  ;  Baek-Hui Kim  ;  Chul-Woo Kim 
Citation
 LUNG CANCER, Vol.71(2) : 209-216, 2011 
Journal Title
LUNG CANCER
ISSN
 0169-5002 
Issue Date
2011
MeSH
Adult ; Aged ; Aged, 80 and over ; Biomarkers, Tumor* ; Carcinoma, Non-Small-Cell Lung/pathology* ; Disease Progression ; Female ; Humans ; Keratin-19/genetics ; Keratin-19/metabolism ; Longitudinal Studies ; Lung Neoplasms/pathology* ; Male ; Middle Aged ; Neoplasm Staging ; Neoplastic Cells, Circulating/metabolism* ; Nuclear Proteins/genetics* ; Nuclear Proteins/metabolism ; Prognosis ; RNA, Messenger/metabolism ; Reproducibility of Results ; Survival Analysis ; Thyroid Nuclear Factor 1 ; Transcription Factors/genetics* ; Transcription Factors/metabolism
Keywords
Circulating tumor cell ; Non-small cell lung cancer ; Thyroid transcription factor-1 ; Cytokeratin 19 ; Real-time reverse transcription-PCR ; Metastasis
Abstract
Circulating tumor cells (CTCs) have been identified in peripheral blood of cancer patients, and reproducible detection of CTCs has demonstrated the potential as useful diagnostic and prognostic tools in several cancers. Present study aimed to determine the clinical relevance of CTCs in surgically resected non-small cell lung cancer (NSCLC) patients. CTC status in presurgery and postsurgery peripheral blood samples from 79 surgically resected NSCLC patients was investigated using thyroid transcription factor-1 (TTF-1) and cytokeratin19 (CK19) mRNA markers by nested real-time RT (reverse transcription)-PCR assay. Detection of TTF-1((+))CTCs was found to be specific to NSCLC patients. TTF-1((+))CTCs were detected in 36.1% (22/61) of patients before surgery and in 37.5% (18/48) after surgery. For CK19 mRNA-expressing CTCs (CK19((+))CTCs), the detection rate was 42.6% (26/61) before surgery, and 25.0% (12/48) after surgery. Cases with postsurgery TTF-1((+)) and/or CK19((+))TCs was more associated with disease progression (P=0.004) and shorter disease progression-free survival (P=0.006) as compared to those without postsurgery CTCs. As an individual marker, postsurgery TTF-1((+))CTCs-positive status was more associated with disease progression (P=0.004) and shorter disease progression-free survival (P=0.004) as compared to postsurgery TTF-1((+))CTCs-negative status. Particularly, patients with postsurgery TTF-1((+))CTCs, but not presurgery (Pre((-))Post((+)) cases) showed marked shorter disease progression-free survival than other patients (P<0.001). On the other hand, a CK19((+))CTC status individually did not show significant clinical relevance, and presurgery CK19((+))CTC status did not either. Present study suggests that TTF-1 mRNA-expressing CTCs might be a useful surrogate predictor of disease progression before clinical manifestations are apparent, and that monitoring of TTF-1((+))CTCs status after surgery may be useful for identifying high-risk patients among surgically resected NSCLC cases.
Full Text
http://www.sciencedirect.com/science/article/pii/S0169500210002047
DOI
10.1016/j.lungcan.2010.04.017
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Pathology (병리학교실) > 1. Journal Papers
Yonsei Authors
Yoon, Sun Och(윤선옥) ORCID logo https://orcid.org/0000-0002-5115-1402
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/92985
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