Cited 84 times in
Contribution of cell elongation to the biofilm formation of Pseudomonas aeruginosa during anaerobic respiration
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 박용진 | - |
dc.contributor.author | 윤상선 | - |
dc.date.accessioned | 2014-12-20T16:21:25Z | - |
dc.date.available | 2014-12-20T16:21:25Z | - |
dc.date.issued | 2011 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/92564 | - |
dc.description.abstract | Pseudomonas aeruginosa, a gram-negative bacterium of clinical importance, forms more robust biofilm during anaerobic respiration, a mode of growth presumed to occur in abnormally thickened mucus layer lining the cystic fibrosis (CF) patient airway. However, molecular basis behind this anaerobiosis-triggered robust biofilm formation is not clearly defined yet. Here, we identified a morphological change naturally accompanied by anaerobic respiration in P. aeruginosa and investigated its effect on the biofilm formation in vitro. A standard laboratory strain, PAO1 was highly elongated during anaerobic respiration compared with bacteria grown aerobically. Microscopic analysis demonstrated that cell elongation likely occurred as a consequence of defective cell division. Cell elongation was dependent on the presence of nitrite reductase (NIR) that reduces nitrite (NO(2) (-)) to nitric oxide (NO) and was repressed in PAO1 in the presence of carboxy-PTIO, a NO antagonist, demonstrating that cell elongation involves a process to respond to NO, a spontaneous byproduct of the anaerobic respiration. Importantly, the non-elongated NIR-deficient mutant failed to form biofilm, while a mutant of nitrate reductase (NAR) and wild type PAO1, both of which were highly elongated, formed robust biofilm. Taken together, our data reveal a role of previously undescribed cell biological event in P. aeruginosa biofilm formation and suggest NIR as a key player involved in such process. | - |
dc.description.statementOfResponsibility | open | - |
dc.format.extent | e16105 | - |
dc.relation.isPartOf | PLOS ONE | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Anaerobiosis | - |
dc.subject.MESH | Biofilms* | - |
dc.subject.MESH | Mutant Proteins | - |
dc.subject.MESH | Nitric Oxide/metabolism | - |
dc.subject.MESH | Nitric Oxide/pharmacology | - |
dc.subject.MESH | Nitrite Reductases/genetics | - |
dc.subject.MESH | Nitrite Reductases/metabolism | - |
dc.subject.MESH | Pseudomonas aeruginosa/cytology* | - |
dc.subject.MESH | Pseudomonas aeruginosa/physiology* | - |
dc.title | Contribution of cell elongation to the biofilm formation of Pseudomonas aeruginosa during anaerobic respiration | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Microbiology (미생물학) | - |
dc.contributor.googleauthor | Mi Young Yoon | - |
dc.contributor.googleauthor | Kang-Mu Lee | - |
dc.contributor.googleauthor | Yongjin Park | - |
dc.contributor.googleauthor | Sang Sun Yoon | - |
dc.identifier.doi | 10.1371/journal.pone.0016105 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A02558 | - |
dc.contributor.localId | A01584 | - |
dc.relation.journalcode | J02540 | - |
dc.identifier.eissn | 1932-6203 | - |
dc.identifier.pmid | 21267455 | - |
dc.contributor.alternativeName | Park, Yong Jin | - |
dc.contributor.alternativeName | Yoon, Sang Sun | - |
dc.contributor.affiliatedAuthor | Yoon, Sang Sun | - |
dc.contributor.affiliatedAuthor | Park, Yong Jin | - |
dc.rights.accessRights | free | - |
dc.citation.volume | 6 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | e16105 | - |
dc.identifier.bibliographicCitation | PLOS ONE, Vol.6(1) : e16105, 2011 | - |
dc.identifier.rimsid | 28636 | - |
dc.type.rims | ART | - |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.