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Contribution of cell elongation to the biofilm formation of Pseudomonas aeruginosa during anaerobic respiration

DC Field Value Language
dc.contributor.author박용진-
dc.contributor.author윤상선-
dc.date.accessioned2014-12-20T16:21:25Z-
dc.date.available2014-12-20T16:21:25Z-
dc.date.issued2011-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/92564-
dc.description.abstractPseudomonas aeruginosa, a gram-negative bacterium of clinical importance, forms more robust biofilm during anaerobic respiration, a mode of growth presumed to occur in abnormally thickened mucus layer lining the cystic fibrosis (CF) patient airway. However, molecular basis behind this anaerobiosis-triggered robust biofilm formation is not clearly defined yet. Here, we identified a morphological change naturally accompanied by anaerobic respiration in P. aeruginosa and investigated its effect on the biofilm formation in vitro. A standard laboratory strain, PAO1 was highly elongated during anaerobic respiration compared with bacteria grown aerobically. Microscopic analysis demonstrated that cell elongation likely occurred as a consequence of defective cell division. Cell elongation was dependent on the presence of nitrite reductase (NIR) that reduces nitrite (NO(2) (-)) to nitric oxide (NO) and was repressed in PAO1 in the presence of carboxy-PTIO, a NO antagonist, demonstrating that cell elongation involves a process to respond to NO, a spontaneous byproduct of the anaerobic respiration. Importantly, the non-elongated NIR-deficient mutant failed to form biofilm, while a mutant of nitrate reductase (NAR) and wild type PAO1, both of which were highly elongated, formed robust biofilm. Taken together, our data reveal a role of previously undescribed cell biological event in P. aeruginosa biofilm formation and suggest NIR as a key player involved in such process.-
dc.description.statementOfResponsibilityopen-
dc.format.extente16105-
dc.relation.isPartOfPLOS ONE-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAnaerobiosis-
dc.subject.MESHBiofilms*-
dc.subject.MESHMutant Proteins-
dc.subject.MESHNitric Oxide/metabolism-
dc.subject.MESHNitric Oxide/pharmacology-
dc.subject.MESHNitrite Reductases/genetics-
dc.subject.MESHNitrite Reductases/metabolism-
dc.subject.MESHPseudomonas aeruginosa/cytology*-
dc.subject.MESHPseudomonas aeruginosa/physiology*-
dc.titleContribution of cell elongation to the biofilm formation of Pseudomonas aeruginosa during anaerobic respiration-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentDept. of Microbiology (미생물학)-
dc.contributor.googleauthorMi Young Yoon-
dc.contributor.googleauthorKang-Mu Lee-
dc.contributor.googleauthorYongjin Park-
dc.contributor.googleauthorSang Sun Yoon-
dc.identifier.doi10.1371/journal.pone.0016105-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA02558-
dc.contributor.localIdA01584-
dc.relation.journalcodeJ02540-
dc.identifier.eissn1932-6203-
dc.identifier.pmid21267455-
dc.contributor.alternativeNamePark, Yong Jin-
dc.contributor.alternativeNameYoon, Sang Sun-
dc.contributor.affiliatedAuthorYoon, Sang Sun-
dc.contributor.affiliatedAuthorPark, Yong Jin-
dc.rights.accessRightsfree-
dc.citation.volume6-
dc.citation.number1-
dc.citation.startPagee16105-
dc.identifier.bibliographicCitationPLOS ONE, Vol.6(1) : e16105, 2011-
dc.identifier.rimsid28636-
dc.type.rimsART-
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Microbiology (미생물학교실) > 1. Journal Papers

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