Cited 73 times in
Rapid detection of Mycobacterium tuberculosis biomarkers in a sandwich immunoassay format using a waveguide-based optical biosensor
DC Field | Value | Language |
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dc.contributor.author | 조상래 | - |
dc.date.accessioned | 2014-12-19T17:44:54Z | - |
dc.date.available | 2014-12-19T17:44:54Z | - |
dc.date.issued | 2012 | - |
dc.identifier.issn | 1472-9792 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/91981 | - |
dc.description.abstract | Early diagnosis of active tuberculosis (TB) remains an elusive challenge, especially in individuals with disseminated TB and HIV co-infection. Recent studies have shown a promise for the direct detection of pathogen-specific biomarkers such as lipoarabinomannan (LAM) for the diagnosis of TB in HIV-positive individuals. Currently, traditional immunoassay platforms that suffer from poor sensitivity and high non-specific interactions are used for the detection of such biomarkers. In this manuscript, we demonstrate the development of sandwich immunoassays for the direct detection of three TB-specific biomarkers, namely LAM, early secretory antigenic target 6 (ESAT6) and antigen 85 complex (Ag85), using a waveguide-based optical biosensor platform. Combining detection within the evanescent field of a planar optical waveguide with functional surfaces that reduce non-specific interactions allows for the ultra-sensitive and quantitative detection of biomarkers (an order of magnitude enhanced sensitivity, as compared to plate-based ELISA) in complex patient samples (urine, serum) within a short time. We also demonstrate the detection of LAM in urine from a small sample of subjects being treated for TB using this approach with excellent sensitivity and 100% corroboration with disease status. These results suggest that pathogen-specific biomarkers can be applied for the rapid and effective diagnosis of disease. It is likely that detection of a combination of biomarkers offers greater reliability of diagnosis, rather than detection of any single pathogen biomarker. | - |
dc.description.statementOfResponsibility | open | - |
dc.relation.isPartOf | TUBERCULOSIS | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Acyltransferases/metabolism* | - |
dc.subject.MESH | Antigens, Bacterial/metabolism* | - |
dc.subject.MESH | Bacterial Proteins/metabolism* | - |
dc.subject.MESH | Biomarkers/metabolism | - |
dc.subject.MESH | Biosensing Techniques* | - |
dc.subject.MESH | Coinfection | - |
dc.subject.MESH | Enzyme-Linked Immunosorbent Assay | - |
dc.subject.MESH | Female | - |
dc.subject.MESH | HIV Seropositivity/epidemiology | - |
dc.subject.MESH | HIV Seropositivity/metabolism* | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Immunoassay | - |
dc.subject.MESH | Lipopolysaccharides/metabolism* | - |
dc.subject.MESH | Male | - |
dc.subject.MESH | Mycobacterium tuberculosis/isolation & purification* | - |
dc.subject.MESH | Mycobacterium tuberculosis/pathogenicity | - |
dc.subject.MESH | Reagent Kits, Diagnostic | - |
dc.subject.MESH | Reproducibility of Results | - |
dc.subject.MESH | Sensitivity and Specificity | - |
dc.subject.MESH | Tuberculosis, Pulmonary/diagnosis | - |
dc.subject.MESH | Tuberculosis, Pulmonary/epidemiology | - |
dc.subject.MESH | Tuberculosis, Pulmonary/metabolism* | - |
dc.title | Rapid detection of Mycobacterium tuberculosis biomarkers in a sandwich immunoassay format using a waveguide-based optical biosensor | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Microbiology (미생물학) | - |
dc.contributor.googleauthor | Harshini Mukundan | - |
dc.contributor.googleauthor | Sandeep Kumar | - |
dc.contributor.googleauthor | Dominique N. Price | - |
dc.contributor.googleauthor | Sonja M. Ray | - |
dc.contributor.googleauthor | Ye-Jin Lee | - |
dc.contributor.googleauthor | Seonyeong Min | - |
dc.contributor.googleauthor | Seokyong Eum | - |
dc.contributor.googleauthor | Jessica Kubicek-Sutherland | - |
dc.contributor.googleauthor | Jesse M. Resnick | - |
dc.contributor.googleauthor | W. Kevin Grace | - |
dc.contributor.googleauthor | Aaron S. Anderson | - |
dc.contributor.googleauthor | Soo Hee Hwang | - |
dc.contributor.googleauthor | Sang Nae Cho | - |
dc.contributor.googleauthor | Laura E. Via | - |
dc.contributor.googleauthor | Clifton Barry III | - |
dc.contributor.googleauthor | Ramamurthy Sakamuri | - |
dc.contributor.googleauthor | Basil I. Swanson | - |
dc.identifier.doi | 10.1016/j.tube.2012.05.009 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A03824 | - |
dc.relation.journalcode | J02760 | - |
dc.identifier.eissn | 1873-281X | - |
dc.identifier.pmid | 22710249 | - |
dc.identifier.url | http://www.sciencedirect.com/science/article/pii/S1472979212001278 | - |
dc.subject.keyword | Tuberculosis | - |
dc.subject.keyword | Diagnosis | - |
dc.subject.keyword | Lipoarabinomannan | - |
dc.subject.keyword | Early secretory antigenic target 6 | - |
dc.subject.keyword | Antigen 85 complex | - |
dc.subject.keyword | Waveguide-based optical biosensor | - |
dc.contributor.alternativeName | Cho, Sang Nae | - |
dc.contributor.affiliatedAuthor | Cho, Sang Nae | - |
dc.citation.volume | 92 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 407 | - |
dc.citation.endPage | 416 | - |
dc.identifier.bibliographicCitation | TUBERCULOSIS, Vol.92(5) : 407-416, 2012 | - |
dc.identifier.rimsid | 30044 | - |
dc.type.rims | ART | - |
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