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Nuclear receptor PPARγ-regulated monoacylglycerol O-acyltransferase 1 (MGAT1) expression is responsible for the lipid accumulation in diet-induced hepatic steatosis

 Yoo Jeong Lee  ;  Eun Hee Ko  ;  Ji Eun Kim  ;  Eunha Kim  ;  Hyemin Lee  ;  Hyeonjin Choi  ;  Jung Hwan Yu  ;  Hyo Jung Kim  ;  Je-Kyung Seong  ;  Kyung-Sup Kim  ;  Jae-woo Kim 
 Proceedings of the National Academy of Sciences of the United States of America, Vol.109(34) : 13656-13661, 2012 
Journal Title
 Proceedings of the National Academy of Sciences of the United States of America 
Issue Date
Recently, hepatic peroxisome proliferator-activated receptor (PPAR)γ has been implicated in hepatic lipid accumulation. We found that the C3H mouse strain does not express PPARγ in the liver and, when subject to a high-fat diet, is resistant to hepatic steatosis, compared with C57BL/6 (B6) mice. Adenoviral PPARγ2 injection into B6 and C3H mice caused hepatic steatosis, and microarray analysis demonstrated that hepatic PPARγ2 expression is associated with genes involved in fatty acid transport and the triglyceride synthesis pathway. In particular, hepatic PPARγ2 expression significantly increased the expression of monoacylglycerol O-acyltransferase 1 (MGAT1). Promoter analysis by luciferase assay and electrophoretic mobility shift assay as well as chromatin immunoprecipitation assay revealed that PPARγ2 directly regulates the MGAT1 promoter activity. The MGAT1 overexpression in cultured hepatocytes enhanced triglyceride synthesis without an increase of PPARγ expression. Importantly, knockdown of MGAT1 in the liver significantly reduced hepatic steatosis in 12-wk-old high-fat-fed mice as well as ob/ob mice, accompanied by weight loss and improved glucose tolerance. These results suggest that the MGAT1 pathway induced by hepatic PPARγ is critically important in the development of hepatic steatosis during diet-induced obesity.
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1. Journal Papers (연구논문) > 1. College of Medicine (의과대학) > Dept. of Biochemistry and Molecular Biology (생화학-분자생물학교실)
Yonsei Authors
김경섭(Kim, Kyung Sup) ORCID logo https://orcid.org/0000-0001-8483-8537
김재우(Kim, Jae Woo) ORCID logo https://orcid.org/0000-0001-5456-9495
김효정(Kim, Hyo Jung) ORCID logo https://orcid.org/0000-0002-3514-1247
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