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Highly pure and expandable PSA-NCAM-positive neural precursors from human ESC and iPSC-derived neural rosettes
DC Field | Value | Language |
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dc.contributor.author | 김한수 | - |
dc.contributor.author | 남택상 | - |
dc.contributor.author | 임중우 | - |
dc.contributor.author | 장지호 | - |
dc.contributor.author | 조성래 | - |
dc.contributor.author | 강훈철 | - |
dc.contributor.author | 김대성 | - |
dc.contributor.author | 김동욱 | - |
dc.date.accessioned | 2014-12-19T17:35:55Z | - |
dc.date.available | 2014-12-19T17:35:55Z | - |
dc.date.issued | 2012 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/91711 | - |
dc.description.abstract | Homogeneous culture of neural precursor cells (NPCs) derived from human pluripotent stem cells (hPSCs) would provide a powerful tool for biomedical applications. However, previous efforts to expand mechanically dissected neural rosettes for cultivation of NPCs remain concerns regarding non-neural cell contamination. In addition, several attempts to purify NPCs using cell surface markers have not demonstrated the expansion capability of the sorted cells. In the present study, we show that polysialic acid-neural cell adhesion molecule (PSA-NCAM) is detected in neural rosette cells derived from hPSCs, and employ PSA-NCAM as a marker for purifying expandable primitive NPCs from the neural rosettes. PSA-NCAM-positive NPCs (termed hNPC(PSA-NCAM+)) were isolated from the heterogeneous cell population of mechanically harvested neural rosettes using magnetic-based cell sorting. The hNPC(PSA-NCAM+) extensively expressed neural markers such as Sox1, Sox2, Nestin, and Musashi-1 (80∼98% of the total cells) and were propagated for multiple passages while retaining their primitive characteristics in our culture condition. Interestingly, PSA-NCAM-negative cells largely exhibited characteristics of neural crest cells. The hNPC(PSA-NCAM+) showed multipotency and responsiveness to instructive cues towards region-specific neuronal subtypes in vitro. When transplanted into the rat striatum, hNPC(PSA-NCAM+) differentiated into neurons, astrocytes, and oligodendrocytes without particular signs of tumorigenesis. Furthermore, Ki67-positive proliferating cells and non-neural lineage cells were rarely detected in the grafts of hNPC(PSA-NCAM+) compared to those of neural rosette cells. Our results suggest that PSA-NCAM-mediated cell isolation provides a highly expandable population of pure primitive NPCs from hPSCs that will lend themselves as a promising strategy for drug screening and cell therapy for neurodegenerative disorders. | - |
dc.description.statementOfResponsibility | open | - |
dc.relation.isPartOf | PLOS ONE | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Brain/cytology | - |
dc.subject.MESH | Cell Culture Techniques | - |
dc.subject.MESH | Cell Differentiation | - |
dc.subject.MESH | Cell Proliferation | - |
dc.subject.MESH | Cell Survival | - |
dc.subject.MESH | Cells, Cultured | - |
dc.subject.MESH | Female | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Immunomagnetic Separation | - |
dc.subject.MESH | Induced Pluripotent Stem Cells/cytology | - |
dc.subject.MESH | Induced Pluripotent Stem Cells/metabolism* | - |
dc.subject.MESH | Induced Pluripotent Stem Cells/transplantation | - |
dc.subject.MESH | Neural Cell Adhesion Molecules/metabolism* | - |
dc.subject.MESH | Neural Stem Cells/cytology | - |
dc.subject.MESH | Neural Stem Cells/metabolism* | - |
dc.subject.MESH | Neural Stem Cells/transplantation | - |
dc.subject.MESH | Neurons/cytology | - |
dc.subject.MESH | Neurons/metabolism | - |
dc.subject.MESH | Rats | - |
dc.subject.MESH | Rats, Sprague-Dawley | - |
dc.subject.MESH | Sialic Acids/metabolism* | - |
dc.subject.MESH | Stem Cell Transplantation | - |
dc.title | Highly pure and expandable PSA-NCAM-positive neural precursors from human ESC and iPSC-derived neural rosettes | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Rehabilitation Medicine (재활의학) | - |
dc.contributor.googleauthor | Dae-Sung Kim | - |
dc.contributor.googleauthor | Dongjin R. Lee | - |
dc.contributor.googleauthor | Han-Soo Kim | - |
dc.contributor.googleauthor | Jeong-Eun Yoo | - |
dc.contributor.googleauthor | Sung Jun Jung | - |
dc.contributor.googleauthor | Bo Young Lim | - |
dc.contributor.googleauthor | Jiho Jang | - |
dc.contributor.googleauthor | Hoon-Chul Kang | - |
dc.contributor.googleauthor | Seungkwon You | - |
dc.contributor.googleauthor | Dong-Youn Hwang | - |
dc.contributor.googleauthor | Joong Woo Leem | - |
dc.contributor.googleauthor | Taick Sang Nam | - |
dc.contributor.googleauthor | Sung-Rae Cho | - |
dc.contributor.googleauthor | Dong-Wook Kim | - |
dc.identifier.doi | 22911689 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A01100 | - |
dc.contributor.localId | A01271 | - |
dc.contributor.localId | A03409 | - |
dc.contributor.localId | A03480 | - |
dc.contributor.localId | A03831 | - |
dc.contributor.localId | A00102 | - |
dc.contributor.localId | A00367 | - |
dc.contributor.localId | A00406 | - |
dc.relation.journalcode | J02540 | - |
dc.identifier.eissn | 1932-6203 | - |
dc.identifier.pmid | 22911689 | - |
dc.subject.keyword | Animals | - |
dc.subject.keyword | Brain/cytology | - |
dc.subject.keyword | Cell Culture Techniques | - |
dc.subject.keyword | Cell Differentiation | - |
dc.subject.keyword | Cell Proliferation | - |
dc.subject.keyword | Cell Survival | - |
dc.subject.keyword | Cells, Cultured | - |
dc.subject.keyword | Female | - |
dc.subject.keyword | Humans | - |
dc.subject.keyword | Immunomagnetic Separation | - |
dc.subject.keyword | Induced Pluripotent Stem Cells/cytology | - |
dc.subject.keyword | Induced Pluripotent Stem Cells/metabolism* | - |
dc.subject.keyword | Induced Pluripotent Stem Cells/transplantation | - |
dc.subject.keyword | Neural Cell Adhesion Molecules/metabolism* | - |
dc.subject.keyword | Neural Stem Cells/cytology | - |
dc.subject.keyword | Neural Stem Cells/metabolism* | - |
dc.subject.keyword | Neural Stem Cells/transplantation | - |
dc.subject.keyword | Neurons/cytology | - |
dc.subject.keyword | Neurons/metabolism | - |
dc.subject.keyword | Rats | - |
dc.subject.keyword | Rats, Sprague-Dawley | - |
dc.subject.keyword | Sialic Acids/metabolism* | - |
dc.subject.keyword | Stem Cell Transplantation | - |
dc.contributor.alternativeName | Kim, Han Soo | - |
dc.contributor.alternativeName | Nam, Taick Sang | - |
dc.contributor.alternativeName | Leem, Joong Woo | - |
dc.contributor.alternativeName | Jang, Ji Ho | - |
dc.contributor.alternativeName | Cho, Sung Rae | - |
dc.contributor.alternativeName | Kang, Hoon Chul | - |
dc.contributor.alternativeName | Kim, Dae Sung | - |
dc.contributor.alternativeName | Kim, Dong Wook | - |
dc.contributor.affiliatedAuthor | Kim, Han Soo | - |
dc.contributor.affiliatedAuthor | Nam, Taick Sang | - |
dc.contributor.affiliatedAuthor | Leem, Joong Woo | - |
dc.contributor.affiliatedAuthor | Jang, Ji Ho | - |
dc.contributor.affiliatedAuthor | Cho, Sung Rae | - |
dc.contributor.affiliatedAuthor | Kang, Hoon Chul | - |
dc.contributor.affiliatedAuthor | Kim, Dae Sung | - |
dc.contributor.affiliatedAuthor | Kim, Dong Wook | - |
dc.citation.volume | 7 | - |
dc.citation.number | 7 | - |
dc.citation.startPage | e39715 | - |
dc.identifier.bibliographicCitation | PLOS ONE, Vol.7(7) : e39715, 2012 | - |
dc.identifier.rimsid | 29571 | - |
dc.type.rims | ART | - |
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