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Relaxin-expressing adenovirus decreases collagen synthesis and up-regulates matrix metalloproteinase expression in keloid fibroblasts: in vitro experiments
DC Field | Value | Language |
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dc.contributor.author | 나동균 | - |
dc.contributor.author | 이원재 | - |
dc.contributor.author | 이주희 | - |
dc.contributor.author | 김용욱 | - |
dc.date.accessioned | 2014-12-19T17:34:40Z | - |
dc.date.available | 2014-12-19T17:34:40Z | - |
dc.date.issued | 2012 | - |
dc.identifier.issn | 0032-1052 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/91671 | - |
dc.description.abstract | BACKGROUND: The hormone relaxin has been shown to affect the extracellular matrix by inhibiting collagen synthesis and expression in fibroblasts stimulated with a profibrotic agent. It also increases matrix metalloproteinase (MMP) expression. To investigate its effect on expression of collagen and MMPs in keloid fibroblasts and human dermal fibroblasts, the authors introduced a relaxin-expressing adenovirus (dE1-RGD/lacZ/RLX) into a human dermal fibroblast cell line and keloid fibroblasts. METHODS: Both fibroblasts were infected with dE1-RGD/lacZ/RLX or control virus, and protein levels of relaxin and secreted transforming growth factor (TGF)-β1 were assessed by enzyme-linked immunosorbent assay, and mRNA levels of collagen type I, collagen type III, MMP-1, and MMP-3 were assessed by real-time reverse-transcriptase polymerase chain reaction and enzyme-linked immunosorbent assay. Expression of Smad3 and phosphorylated Smad3 was also examined, and relaxin's effect on Smad2/3 complex localization was evaluated. RESULTS: When human dermal fibroblasts and keloid fibroblasts were transduced with dE1-RGD/lacZ/RLX or dE1-RGD/lacZ (control), mRNA expression of type I and type III collagen was markedly decreased by relaxin regardless of TGF-β (10 ng/ml) treatment. Expression of Smad3 and phosphorylated Smad3 was reduced in keloid fibroblasts and decreased translocation of Smad 2/3 complex from cytosols to the nucleus of the human dermal fibroblasts with TGF-β after dE1-RGD/lacZ/RLX transduction, suggesting that relaxin reduces collagen synthesis by blocking TGF-β signaling. Analyses revealed that MMP-1 and MMP-3 expression were significantly increased in human dermal fibroblasts and keloid fibroblasts after dE1-RGD/lacZ/RLX transduction. CONCLUSION: These results suggest that the antifibrotic effect of relaxin-expressing adenovirus may have therapeutic effects on keloids. | - |
dc.description.statementOfResponsibility | open | - |
dc.relation.isPartOf | PLASTIC AND RECONSTRUCTIVE SURGERY | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Adenoviridae/metabolism* | - |
dc.subject.MESH | Cell Line | - |
dc.subject.MESH | Cells, Cultured | - |
dc.subject.MESH | Collagen/biosynthesis* | - |
dc.subject.MESH | Dermis/cytology | - |
dc.subject.MESH | Dermis/metabolism | - |
dc.subject.MESH | Dermis/virology | - |
dc.subject.MESH | Extracellular Matrix/metabolism* | - |
dc.subject.MESH | Fibroblasts/metabolism | - |
dc.subject.MESH | Fibroblasts/virology* | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Keloid/metabolism | - |
dc.subject.MESH | Keloid/pathology | - |
dc.subject.MESH | Keloid/virology* | - |
dc.subject.MESH | Metalloproteases/metabolism* | - |
dc.subject.MESH | Relaxin/secretion* | - |
dc.subject.MESH | Smad3 Protein/metabolism | - |
dc.subject.MESH | Up-Regulation | - |
dc.title | Relaxin-expressing adenovirus decreases collagen synthesis and up-regulates matrix metalloproteinase expression in keloid fibroblasts: in vitro experiments | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Dept. of Dermatology (피부과학) | - |
dc.contributor.googleauthor | Lee, Won Jai | - |
dc.contributor.googleauthor | Choi, Il-Kyu | - |
dc.contributor.googleauthor | Lee, Ju Hee | - |
dc.contributor.googleauthor | Lee, Jung-Sun | - |
dc.contributor.googleauthor | Kim, Yong Oock | - |
dc.contributor.googleauthor | Rah, Dong Kyun | - |
dc.contributor.googleauthor | Yun, Chae-Ok | - |
dc.identifier.doi | 22929264 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A01229 | - |
dc.contributor.localId | A03005 | - |
dc.contributor.localId | A00749 | - |
dc.contributor.localId | A03171 | - |
dc.relation.journalcode | J02534 | - |
dc.identifier.eissn | 1529-4242 | - |
dc.identifier.pmid | 22929264 | - |
dc.identifier.url | http://ovidsp.ovid.com/ovidweb.cgi?T=JS&CSC=Y&NEWS=N&PAGE=fulltext&AN=00006534-201209000-00007&LSLINK=80&D=ovft | - |
dc.subject.keyword | Adenoviridae/metabolism* | - |
dc.subject.keyword | Cell Line | - |
dc.subject.keyword | Cells, Cultured | - |
dc.subject.keyword | Collagen/biosynthesis* | - |
dc.subject.keyword | Dermis/cytology | - |
dc.subject.keyword | Dermis/metabolism | - |
dc.subject.keyword | Dermis/virology | - |
dc.subject.keyword | Extracellular Matrix/metabolism* | - |
dc.subject.keyword | Fibroblasts/metabolism | - |
dc.subject.keyword | Fibroblasts/virology* | - |
dc.subject.keyword | Humans | - |
dc.subject.keyword | Keloid/metabolism | - |
dc.subject.keyword | Keloid/pathology | - |
dc.subject.keyword | Keloid/virology* | - |
dc.subject.keyword | Metalloproteases/metabolism* | - |
dc.subject.keyword | Relaxin/secretion* | - |
dc.subject.keyword | Smad3 Protein/metabolism | - |
dc.subject.keyword | Up-Regulation | - |
dc.contributor.alternativeName | Rah, Dong Kyun | - |
dc.contributor.alternativeName | Lee, Won Jai | - |
dc.contributor.alternativeName | Lee, Ju Hee | - |
dc.contributor.alternativeName | Kim, Yong Oock | - |
dc.contributor.affiliatedAuthor | Rah, Dong Kyun | - |
dc.contributor.affiliatedAuthor | Lee, Won Jai | - |
dc.contributor.affiliatedAuthor | Kim, Yong Oock | - |
dc.contributor.affiliatedAuthor | Lee, Ju Hee | - |
dc.citation.volume | 130 | - |
dc.citation.number | 3 | - |
dc.citation.startPage | 407 | - |
dc.citation.endPage | 417 | - |
dc.identifier.bibliographicCitation | PLASTIC AND RECONSTRUCTIVE SURGERY, Vol.130(3) : 407-417, 2012 | - |
dc.identifier.rimsid | 29551 | - |
dc.type.rims | ART | - |
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