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Molecular characterization of Clonorchis sinensis tetraspanin 2 extracellular loop 2

Authors
 Tae Yun Kim  ;  Eun Joo Chung  ;  Woon-Mok Sohn  ;  Sung-Hong Hong  ;  Tai-Soon Yong 
Citation
 PARASITOLOGY RESEARCH, Vol.110(2) : 707-711, 2012 
Journal Title
 PARASITOLOGY RESEARCH 
ISSN
 0932-0113 
Issue Date
2012
MeSH
Amino Acid Motifs ; Animals ; Antibodies, Helminth/blood ; Chromatography, Affinity ; Cloning, Molecular ; Clonorchiasis/immunology ; Clonorchis sinensis/genetics* ; Conserved Sequence ; Gene Expression ; Helminth Proteins/genetics* ; Humans ; Recombinant Fusion Proteins/genetics ; Recombinant Fusion Proteins/isolation & purification ; Recombinant Fusion Proteins/metabolism ; Tetraspanins/genetics*
Keywords
Schistosoma ; Extracellular Loop ; Human Seron ; Cysticercosis ; Liver Fluke
Abstract
In the course of examining EST of the liver fluke Clonorchis sinensis, a cDNA encoding the protein similar to tetraspanin 2 (TSP-2) of blood fluke schistosome was identified as CsTSP2. TSPs are a family of eukaryotic cell membrane-spanning proteins thought to anchor multiple proteins to one area of the cell membrane. Multiple sequence alignment of CsTSP2 revealed over 40% of identities with those of schistosomes. The CCC, PXSC, and CG motives characteristic to extracellular loop 2 (EC2) region of TSP-2 were well conserved. PCR-amplified EC2 of CsTSP2 (CsTSP2EC2) was subcloned into pEXP5 NT/TOPO bacterial expression plasmid vector. Recombinant CsTSP2EC2 protein (rCsTSP2EC2) fused with 6X His tag was overexpressed bacterially and purified by using Ni-NTA affinity chromatography under denaturing condition. The purified rCsTSP2EC2 was recognized specifically by the sera from human infected with C. sinensis. Considering biological role and specific immunity of TSPs, rCsTSP2EC2 might be a probable vaccine candidate against clonorchiasis. Protective effects of immunizing rCsTSP2EC2 should be further studied
Full Text
http://link.springer.com/article/10.1007%2Fs00436-011-2546-6
DOI
21842393
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Environmental Medical Biology (환경의생물학교실) > 1. Journal Papers
Yonsei Authors
Kim,​ Tae Yun(김태윤)
Yong, Tai Soon(용태순) ORCID logo https://orcid.org/0000-0002-3445-0769
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/91638
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