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Apoptosis occurs differentially according to glomerular size in diabetic kidney disease

DC FieldValueLanguage
dc.contributor.author강신욱-
dc.contributor.author강혜영-
dc.contributor.author곽승재-
dc.contributor.author남보영-
dc.contributor.author박정탁-
dc.contributor.author유태현-
dc.contributor.author이순하-
dc.contributor.author장태익-
dc.contributor.author정동섭-
dc.contributor.author한승혁-
dc.date.accessioned2014-12-19T17:29:43Z-
dc.date.available2014-12-19T17:29:43Z-
dc.date.issued2012-
dc.identifier.issn0931-0509-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/91514-
dc.description.abstractBACKGROUND: Apoptosis; which is involved in the process of mesangial cell and podocyte loss in diabetic nephropathy; is known to be regulated by protein kinase B/Akt (Akt). A number of studies have therefore investigated the activity of Akt under diabetic conditions; but the results have not been consistent. In this study; we hypothesized that apoptosis may occur differentially and that Akt may be differentially activated according to glomerular size in diabetic kidney disease. METHODS: Fifty male Sprague-Dawley rats were injected intraperitoneally with diluent (C; n = 25) or streptozotocin (DM; n = 25). After 3 months; glomeruli were isolated using sieves with pore sizes of 250; 150; 125 and 75 μm and then classified into large glomeruli (on the 125-μm sieve; LG) and small glomeruli (on the 75-μm sieve; SG) groups. Western blot analyses for phospho-Akt; apoptosis-related molecules (Bax; Bcl-2; active fragments of Caspase-3 and phospho-p53) and cyclin-dependent kinase inhibitors were performed. CONCLUSIONS: The numbers of total cells and podocytes in isolated glomeruli were determined using transmission electron microscopy. Akt phosphorylation was significantly decreased in DM-LG; while it was significantly increased in DM-SG (P < 0.05). The ratio of Bax/Bcl-2 protein expression and active fragments of Caspase-3 and phospho-p53 protein expression were significantly increased in DM-LG compared to DM-SG and C-SG (P < 0.001 and P < 0.01; respectively). In contrast; the expression of p27(Kip1) and p21(Cip1) was significantly increased in DM-SG compared to DM-LG and C-SG (P < 0.05). The numbers of total glomerular cells and podocytes were significantly decreased in DM-LG (P < 0.05). In conclusion; these data show differential expression of Akt activity and apoptosis-related molecules according to glomerular size in diabetic nephropathy; suggesting that apoptosis may be more operative in more hypertrophic glomeruli; resulting in fewer glomerular cells and podocytes in diabetic nephropathy.-
dc.description.statementOfResponsibilityopen-
dc.relation.isPartOfNEPHROLOGY DIALYSIS TRANSPLANTATION-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.subject.MESHAnimals-
dc.subject.MESHApoptosis*-
dc.subject.MESHBlotting, Western-
dc.subject.MESHCaspase 3/metabolism-
dc.subject.MESHDiabetes Mellitus, Experimental/metabolism-
dc.subject.MESHDiabetes Mellitus, Experimental/pathology*-
dc.subject.MESHDiabetic Nephropathies/metabolism-
dc.subject.MESHDiabetic Nephropathies/pathology*-
dc.subject.MESHFluorescent Antibody Technique-
dc.subject.MESHKidney Glomerulus/metabolism-
dc.subject.MESHKidney Glomerulus/pathology*-
dc.subject.MESHMale-
dc.subject.MESHPhosphorylation-
dc.subject.MESHPodocytes/metabolism-
dc.subject.MESHPodocytes/pathology*-
dc.subject.MESHProto-Oncogene Proteins c-akt/metabolism-
dc.subject.MESHProto-Oncogene Proteins c-bcl-2/metabolism-
dc.subject.MESHRats-
dc.subject.MESHRats, Sprague-Dawley-
dc.subject.MESHbcl-2-Associated X Protein/metabolism-
dc.titleApoptosis occurs differentially according to glomerular size in diabetic kidney disease-
dc.typeArticle-
dc.contributor.collegeCollege of Medicine (의과대학)-
dc.contributor.departmentMedical Research Center (임상의학연구센터)-
dc.contributor.googleauthorDong-Sub Jung-
dc.contributor.googleauthorSun Ha Lee-
dc.contributor.googleauthorSeung-Jae Kwak-
dc.contributor.googleauthorJin Ji Li-
dc.contributor.googleauthorDo Hee Kim-
dc.contributor.googleauthorBo-Young Nam-
dc.contributor.googleauthorHye Young Kang-
dc.contributor.googleauthorTae Ik Chang-
dc.contributor.googleauthorJung Tak Park-
dc.contributor.googleauthorSeung Hyeok Han-
dc.contributor.googleauthorTae-Hyun Yoo-
dc.contributor.googleauthorShin-Wook Kang-
dc.identifier.doi21652547-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA00053-
dc.contributor.localIdA00096-
dc.contributor.localIdA00170-
dc.contributor.localIdA01251-
dc.contributor.localIdA01654-
dc.contributor.localIdA02526-
dc.contributor.localIdA02908-
dc.contributor.localIdA03486-
dc.contributor.localIdA03594-
dc.contributor.localIdA04304-
dc.relation.journalcodeJ02316-
dc.identifier.eissn1460-2385-
dc.identifier.pmid21652547-
dc.identifier.urlhttp://ndt.oxfordjournals.org/content/27/1/259.long-
dc.subject.keywordAnimals-
dc.subject.keywordApoptosis*-
dc.subject.keywordBlotting, Western-
dc.subject.keywordCaspase 3/metabolism-
dc.subject.keywordDiabetes Mellitus, Experimental/metabolism-
dc.subject.keywordDiabetes Mellitus, Experimental/pathology*-
dc.subject.keywordDiabetic Nephropathies/metabolism-
dc.subject.keywordDiabetic Nephropathies/pathology*-
dc.subject.keywordFluorescent Antibody Technique-
dc.subject.keywordKidney Glomerulus/metabolism-
dc.subject.keywordKidney Glomerulus/pathology*-
dc.subject.keywordMale-
dc.subject.keywordPhosphorylation-
dc.subject.keywordPodocytes/metabolism-
dc.subject.keywordPodocytes/pathology*-
dc.subject.keywordProto-Oncogene Proteins c-akt/metabolism-
dc.subject.keywordProto-Oncogene Proteins c-bcl-2/metabolism-
dc.subject.keywordRats-
dc.subject.keywordRats, Sprague-Dawley-
dc.subject.keywordbcl-2-Associated X Protein/metabolism-
dc.contributor.alternativeNameKang, Shin Wook-
dc.contributor.alternativeNameKang, Hye Young-
dc.contributor.alternativeNameKwak, Seung Jae-
dc.contributor.alternativeNameNam, Bo Young-
dc.contributor.alternativeNamePark, Jung Tak-
dc.contributor.alternativeNameYoo, Tae Hyun-
dc.contributor.alternativeNameLee, Sun Ha-
dc.contributor.alternativeNameChang, Tae Ik-
dc.contributor.alternativeNameJung, Dong Sub-
dc.contributor.alternativeNameHan, Seung Hyeok-
dc.contributor.affiliatedAuthorKang, Shin Wook-
dc.contributor.affiliatedAuthorKang, Hye Young-
dc.contributor.affiliatedAuthorKwak, Seung Jae-
dc.contributor.affiliatedAuthorNam, Bo Young-
dc.contributor.affiliatedAuthorPark, Jung Tak-
dc.contributor.affiliatedAuthorYoo, Tae Hyun-
dc.contributor.affiliatedAuthorLee, Sun Ha-
dc.contributor.affiliatedAuthorChang, Tae Ik-
dc.contributor.affiliatedAuthorJung, Dong Sub-
dc.contributor.affiliatedAuthorHan, Seung Hyeok-
dc.citation.volume27-
dc.citation.number1-
dc.citation.startPage259-
dc.citation.endPage266-
dc.identifier.bibliographicCitationNEPHROLOGY DIALYSIS TRANSPLANTATION, Vol.27(1) : 259-266, 2012-
Appears in Collections:
1. College of Medicine (의과대학) > Medical Research Center (임상의학연구센터) > 1. Journal Papers
1. College of Medicine (의과대학) > Dept. of Internal Medicine (내과학교실) > 1. Journal Papers
1. College of Medicine (의과대학) > Yonsei Biomedical Research Center (연세의생명연구원) > 1. Journal Papers

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