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Apoptosis occurs differentially according to glomerular size in diabetic kidney disease
DC Field | Value | Language |
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dc.contributor.author | 강신욱 | - |
dc.contributor.author | 강혜영 | - |
dc.contributor.author | 곽승재 | - |
dc.contributor.author | 남보영 | - |
dc.contributor.author | 박정탁 | - |
dc.contributor.author | 유태현 | - |
dc.contributor.author | 이순하 | - |
dc.contributor.author | 장태익 | - |
dc.contributor.author | 정동섭 | - |
dc.contributor.author | 한승혁 | - |
dc.date.accessioned | 2014-12-19T17:29:43Z | - |
dc.date.available | 2014-12-19T17:29:43Z | - |
dc.date.issued | 2012 | - |
dc.identifier.issn | 0931-0509 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/91514 | - |
dc.description.abstract | BACKGROUND: Apoptosis; which is involved in the process of mesangial cell and podocyte loss in diabetic nephropathy; is known to be regulated by protein kinase B/Akt (Akt). A number of studies have therefore investigated the activity of Akt under diabetic conditions; but the results have not been consistent. In this study; we hypothesized that apoptosis may occur differentially and that Akt may be differentially activated according to glomerular size in diabetic kidney disease. METHODS: Fifty male Sprague-Dawley rats were injected intraperitoneally with diluent (C; n = 25) or streptozotocin (DM; n = 25). After 3 months; glomeruli were isolated using sieves with pore sizes of 250; 150; 125 and 75 μm and then classified into large glomeruli (on the 125-μm sieve; LG) and small glomeruli (on the 75-μm sieve; SG) groups. Western blot analyses for phospho-Akt; apoptosis-related molecules (Bax; Bcl-2; active fragments of Caspase-3 and phospho-p53) and cyclin-dependent kinase inhibitors were performed. CONCLUSIONS: The numbers of total cells and podocytes in isolated glomeruli were determined using transmission electron microscopy. Akt phosphorylation was significantly decreased in DM-LG; while it was significantly increased in DM-SG (P < 0.05). The ratio of Bax/Bcl-2 protein expression and active fragments of Caspase-3 and phospho-p53 protein expression were significantly increased in DM-LG compared to DM-SG and C-SG (P < 0.001 and P < 0.01; respectively). In contrast; the expression of p27(Kip1) and p21(Cip1) was significantly increased in DM-SG compared to DM-LG and C-SG (P < 0.05). The numbers of total glomerular cells and podocytes were significantly decreased in DM-LG (P < 0.05). In conclusion; these data show differential expression of Akt activity and apoptosis-related molecules according to glomerular size in diabetic nephropathy; suggesting that apoptosis may be more operative in more hypertrophic glomeruli; resulting in fewer glomerular cells and podocytes in diabetic nephropathy. | - |
dc.description.statementOfResponsibility | open | - |
dc.relation.isPartOf | NEPHROLOGY DIALYSIS TRANSPLANTATION | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Apoptosis* | - |
dc.subject.MESH | Blotting, Western | - |
dc.subject.MESH | Caspase 3/metabolism | - |
dc.subject.MESH | Diabetes Mellitus, Experimental/metabolism | - |
dc.subject.MESH | Diabetes Mellitus, Experimental/pathology* | - |
dc.subject.MESH | Diabetic Nephropathies/metabolism | - |
dc.subject.MESH | Diabetic Nephropathies/pathology* | - |
dc.subject.MESH | Fluorescent Antibody Technique | - |
dc.subject.MESH | Kidney Glomerulus/metabolism | - |
dc.subject.MESH | Kidney Glomerulus/pathology* | - |
dc.subject.MESH | Male | - |
dc.subject.MESH | Phosphorylation | - |
dc.subject.MESH | Podocytes/metabolism | - |
dc.subject.MESH | Podocytes/pathology* | - |
dc.subject.MESH | Proto-Oncogene Proteins c-akt/metabolism | - |
dc.subject.MESH | Proto-Oncogene Proteins c-bcl-2/metabolism | - |
dc.subject.MESH | Rats | - |
dc.subject.MESH | Rats, Sprague-Dawley | - |
dc.subject.MESH | bcl-2-Associated X Protein/metabolism | - |
dc.title | Apoptosis occurs differentially according to glomerular size in diabetic kidney disease | - |
dc.type | Article | - |
dc.contributor.college | College of Medicine (의과대학) | - |
dc.contributor.department | Medical Research Center (임상의학연구센터) | - |
dc.contributor.googleauthor | Dong-Sub Jung | - |
dc.contributor.googleauthor | Sun Ha Lee | - |
dc.contributor.googleauthor | Seung-Jae Kwak | - |
dc.contributor.googleauthor | Jin Ji Li | - |
dc.contributor.googleauthor | Do Hee Kim | - |
dc.contributor.googleauthor | Bo-Young Nam | - |
dc.contributor.googleauthor | Hye Young Kang | - |
dc.contributor.googleauthor | Tae Ik Chang | - |
dc.contributor.googleauthor | Jung Tak Park | - |
dc.contributor.googleauthor | Seung Hyeok Han | - |
dc.contributor.googleauthor | Tae-Hyun Yoo | - |
dc.contributor.googleauthor | Shin-Wook Kang | - |
dc.identifier.doi | 21652547 | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A00053 | - |
dc.contributor.localId | A00096 | - |
dc.contributor.localId | A00170 | - |
dc.contributor.localId | A01251 | - |
dc.contributor.localId | A01654 | - |
dc.contributor.localId | A02526 | - |
dc.contributor.localId | A02908 | - |
dc.contributor.localId | A03486 | - |
dc.contributor.localId | A03594 | - |
dc.contributor.localId | A04304 | - |
dc.relation.journalcode | J02316 | - |
dc.identifier.eissn | 1460-2385 | - |
dc.identifier.pmid | 21652547 | - |
dc.identifier.url | http://ndt.oxfordjournals.org/content/27/1/259.long | - |
dc.subject.keyword | Animals | - |
dc.subject.keyword | Apoptosis* | - |
dc.subject.keyword | Blotting, Western | - |
dc.subject.keyword | Caspase 3/metabolism | - |
dc.subject.keyword | Diabetes Mellitus, Experimental/metabolism | - |
dc.subject.keyword | Diabetes Mellitus, Experimental/pathology* | - |
dc.subject.keyword | Diabetic Nephropathies/metabolism | - |
dc.subject.keyword | Diabetic Nephropathies/pathology* | - |
dc.subject.keyword | Fluorescent Antibody Technique | - |
dc.subject.keyword | Kidney Glomerulus/metabolism | - |
dc.subject.keyword | Kidney Glomerulus/pathology* | - |
dc.subject.keyword | Male | - |
dc.subject.keyword | Phosphorylation | - |
dc.subject.keyword | Podocytes/metabolism | - |
dc.subject.keyword | Podocytes/pathology* | - |
dc.subject.keyword | Proto-Oncogene Proteins c-akt/metabolism | - |
dc.subject.keyword | Proto-Oncogene Proteins c-bcl-2/metabolism | - |
dc.subject.keyword | Rats | - |
dc.subject.keyword | Rats, Sprague-Dawley | - |
dc.subject.keyword | bcl-2-Associated X Protein/metabolism | - |
dc.contributor.alternativeName | Kang, Shin Wook | - |
dc.contributor.alternativeName | Kang, Hye Young | - |
dc.contributor.alternativeName | Kwak, Seung Jae | - |
dc.contributor.alternativeName | Nam, Bo Young | - |
dc.contributor.alternativeName | Park, Jung Tak | - |
dc.contributor.alternativeName | Yoo, Tae Hyun | - |
dc.contributor.alternativeName | Lee, Sun Ha | - |
dc.contributor.alternativeName | Chang, Tae Ik | - |
dc.contributor.alternativeName | Jung, Dong Sub | - |
dc.contributor.alternativeName | Han, Seung Hyeok | - |
dc.contributor.affiliatedAuthor | Kang, Shin Wook | - |
dc.contributor.affiliatedAuthor | Kang, Hye Young | - |
dc.contributor.affiliatedAuthor | Kwak, Seung Jae | - |
dc.contributor.affiliatedAuthor | Nam, Bo Young | - |
dc.contributor.affiliatedAuthor | Park, Jung Tak | - |
dc.contributor.affiliatedAuthor | Yoo, Tae Hyun | - |
dc.contributor.affiliatedAuthor | Lee, Sun Ha | - |
dc.contributor.affiliatedAuthor | Chang, Tae Ik | - |
dc.contributor.affiliatedAuthor | Jung, Dong Sub | - |
dc.contributor.affiliatedAuthor | Han, Seung Hyeok | - |
dc.citation.volume | 27 | - |
dc.citation.number | 1 | - |
dc.citation.startPage | 259 | - |
dc.citation.endPage | 266 | - |
dc.identifier.bibliographicCitation | NEPHROLOGY DIALYSIS TRANSPLANTATION, Vol.27(1) : 259-266, 2012 | - |
dc.identifier.rimsid | 31324 | - |
dc.type.rims | ART | - |
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