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A single serum dilution enzyme-linked immunosorbent assay for determining anti-human papillomavirus (HPV) antibody titres in humans immunised with prophylactic HPV vaccines

Authors
 Yingji Jin  ;  Hyoung Jin Kim  ;  Ga Won Yim  ;  Young Tae Kim  ;  Don Yong Chang  ;  Hong-Jin Kima 
Citation
 JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, Vol.66 : 352-355, 2012 
Journal Title
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
ISSN
 0731-7085 
Issue Date
2012
MeSH
Adult ; Aged ; Child ; Child, Preschool ; Enzyme-Linked Immunosorbent Assay/methods* ; Female ; Human papillomavirus 16/immunology* ; Human papillomavirus 18/immunology* ; Humans ; Infant ; Middle Aged ; Papillomavirus Vaccines/administration & dosage ; Papillomavirus Vaccines/immunology* ; Reproducibility of Results ; Young Adult
Keywords
Enzyme-linked immunosorbent assay ; Human papillomavirus ; Serology ; Virus-like particle
Abstract
Two types of prophylactic human papillomavirus (HPV) vaccines are currently available. However, there is no simple monitoring system for assessing acquired immunity that can cope simultaneously with large numbers of serum samples. Approximately 30% of women with normal cytology are known to be seropositive for HPV types 16 and 18 because of the high prevalence of these HPV types. Therefore, to be useful the monitoring system has to discriminate clearly between vaccine recipients and other serology groups. However, there has never been any focus on developing a method to satisfy this condition. In this study, we developed a high-throughput single-serum-dilution enzyme-linked immunoassay (ELISA) system for determining anti-HPV antibody titres following vaccination. We optimised the conditions for each ELISA step to increase its accuracy and precision and to avoid the high background of non-specific reactions that is a major problem for serology assays. The new ELISA system has superior linearity, accuracy and reproducibility. Moreover, it clearly discriminated between antibody levels in vaccine recipients and those in other serology groups such as individuals with normal cervical cytology and those with cervical cancer. Therefore, this single-serum-dilution ELISA should be very useful for assessing the acquired immunity of HPV vaccine recipients
Full Text
http://www.sciencedirect.com/science/article/pii/S0731708512001343
DOI
22469434
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Obstetrics and Gynecology (산부인과학교실) > 1. Journal Papers
Yonsei Authors
Kim, Young Tae(김영태) ORCID logo https://orcid.org/0000-0002-7347-1052
Yim, Ga Won(임가원)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/91059
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