Cited 18 times in
Dose- and time-dependent effects of recombinant human bone morphogenetic protein-2 on the osteogenic and adipogenic potentials of alveolar bone-derived stromal cells
DC Field | Value | Language |
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dc.contributor.author | 김창성 | - |
dc.contributor.author | 박정철 | - |
dc.contributor.author | 조규성 | - |
dc.date.accessioned | 2014-12-19T17:14:58Z | - |
dc.date.available | 2014-12-19T17:14:58Z | - |
dc.date.issued | 2012 | - |
dc.identifier.issn | 0022-3484 | - |
dc.identifier.uri | https://ir.ymlib.yonsei.ac.kr/handle/22282913/91058 | - |
dc.description.abstract | BACKGROUND AND OBJECTIVE: Recombinant human bone morphogenetic protein-2 (rhBMP-2) is a well-known growth factor that can induce robust bone formation, and recent studies have shown that rhBMP-2-induced osteogenesis is closely related to adipogenesis. The aim of the present study was to determine the dose- and time-dependent effects of rhBMP-2 on the osteogenic and adipogenic differentiation of human alveolar bone-derived stromal cells (hABCs) in vivo and in vitro. MATERIAL AND METHODS: hABCs were isolated and cultured, and then transplanted using a carrier treated either with or without rhBMP-2 (100 μg/mL) into an ectopic subcutaneous mouse model. Comprehensive histologic and histometric analyses were performed after an 8-wk healing period. To further understand the dose-dependent (0, 10, 50, 200, 500 and 1000 ng/mL) and time-dependent (0, 3, 5, 7 and 14 d) effects of rhBMP-2 on osteogenic and adipogenic differentiation, in vitro osteogenic and adipogenic differentiation of hABCs were evaluated, and the expression of related mRNAs, including those for alkaline phosphatase, osteocalcin, bone sialoprotein, peroxisome-proliferator-activated receptor gamma-2 and lipoprotein lipase, were assessed using quantitative RT-PCR. RESULTS: rhBMP-2 significantly promoted the osteogenic and adipogenic differentiation of hABCs in vivo, and gradually increased both the osteogenic and adipogenic potential in a dose- and time-dependent manner with minimal deviation in vitro. The expression of osteogenesis- and adipogenesis-associated mRNAs were concomitantly up-regulated by rhBMP-2. CONCLUSION: The findings of the present study showed that rhBMP-2 significantly enhanced the adipogenic as well as the osteogenic potential of hABCs in dose- and time-dependent manner. The control of adipogenic differentiation of hABCs should be considered when regenerating the alveolar bone using rhBMP-2. | - |
dc.description.statementOfResponsibility | open | - |
dc.relation.isPartOf | JOURNAL OF PERIODONTAL RESEARCH | - |
dc.rights | CC BY-NC-ND 2.0 KR | - |
dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/2.0/kr/ | - |
dc.title | Dose- and time-dependent effects of recombinant human bone morphogenetic protein-2 on the osteogenic and adipogenic potentials of alveolar bone-derived stromal cells | - |
dc.type | Article | - |
dc.contributor.college | College of Dentistry (치과대학) | - |
dc.contributor.department | Dept. of Periodontology (치주과학) | - |
dc.contributor.googleauthor | J-C. Park | - |
dc.contributor.googleauthor | J.C. Kim | - |
dc.contributor.googleauthor | B-K. Kim | - |
dc.contributor.googleauthor | K-S. Cho | - |
dc.contributor.googleauthor | G-I. Im | - |
dc.contributor.googleauthor | B-S. Kim | - |
dc.contributor.googleauthor | C-S. Kim | - |
dc.identifier.doi | 10.1111/j.1600-0765.2012.01477.x | - |
dc.admin.author | false | - |
dc.admin.mapping | false | - |
dc.contributor.localId | A01041 | - |
dc.contributor.localId | A01653 | - |
dc.contributor.localId | A03810 | - |
dc.relation.journalcode | J01696 | - |
dc.identifier.eissn | 1600-0765 | - |
dc.identifier.pmid | adipogenesis ; osteogenesis ; recombinant human bone morphogeneticprotein-2 ; stem cells ; tissue engineering | - |
dc.identifier.url | http://onlinelibrary.wiley.com/doi/10.1111/j.1600-0765.2012.01477.x/abstract | - |
dc.subject.keyword | adipogenesis | - |
dc.subject.keyword | osteogenesis | - |
dc.subject.keyword | recombinant human bone morphogeneticprotein-2 | - |
dc.subject.keyword | stem cells | - |
dc.subject.keyword | tissue engineering | - |
dc.contributor.alternativeName | Kim, Chang Sung | - |
dc.contributor.alternativeName | Park, Jung Chul | - |
dc.contributor.alternativeName | Cho, Kyoo Sung | - |
dc.contributor.affiliatedAuthor | Kim, Chang Sung | - |
dc.contributor.affiliatedAuthor | Park, Jung Chul | - |
dc.contributor.affiliatedAuthor | Cho, Kyoo Sung | - |
dc.citation.volume | 47 | - |
dc.citation.number | 5 | - |
dc.citation.startPage | 645 | - |
dc.citation.endPage | 654 | - |
dc.identifier.bibliographicCitation | JOURNAL OF PERIODONTAL RESEARCH, Vol.47(5) : 645-654, 2012 | - |
dc.identifier.rimsid | 33306 | - |
dc.type.rims | ART | - |
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