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Dose- and time-dependent effects of recombinant human bone morphogenetic protein-2 on the osteogenic and adipogenic potentials of alveolar bone-derived stromal cells

DC FieldValueLanguage
dc.contributor.author김창성-
dc.contributor.author박정철-
dc.contributor.author조규성-
dc.date.accessioned2014-12-19T17:14:58Z-
dc.date.available2014-12-19T17:14:58Z-
dc.date.issued2012-
dc.identifier.issn0022-3484-
dc.identifier.urihttps://ir.ymlib.yonsei.ac.kr/handle/22282913/91058-
dc.description.abstractBACKGROUND AND OBJECTIVE: Recombinant human bone morphogenetic protein-2 (rhBMP-2) is a well-known growth factor that can induce robust bone formation, and recent studies have shown that rhBMP-2-induced osteogenesis is closely related to adipogenesis. The aim of the present study was to determine the dose- and time-dependent effects of rhBMP-2 on the osteogenic and adipogenic differentiation of human alveolar bone-derived stromal cells (hABCs) in vivo and in vitro. MATERIAL AND METHODS: hABCs were isolated and cultured, and then transplanted using a carrier treated either with or without rhBMP-2 (100 μg/mL) into an ectopic subcutaneous mouse model. Comprehensive histologic and histometric analyses were performed after an 8-wk healing period. To further understand the dose-dependent (0, 10, 50, 200, 500 and 1000 ng/mL) and time-dependent (0, 3, 5, 7 and 14 d) effects of rhBMP-2 on osteogenic and adipogenic differentiation, in vitro osteogenic and adipogenic differentiation of hABCs were evaluated, and the expression of related mRNAs, including those for alkaline phosphatase, osteocalcin, bone sialoprotein, peroxisome-proliferator-activated receptor gamma-2 and lipoprotein lipase, were assessed using quantitative RT-PCR. RESULTS: rhBMP-2 significantly promoted the osteogenic and adipogenic differentiation of hABCs in vivo, and gradually increased both the osteogenic and adipogenic potential in a dose- and time-dependent manner with minimal deviation in vitro. The expression of osteogenesis- and adipogenesis-associated mRNAs were concomitantly up-regulated by rhBMP-2. CONCLUSION: The findings of the present study showed that rhBMP-2 significantly enhanced the adipogenic as well as the osteogenic potential of hABCs in dose- and time-dependent manner. The control of adipogenic differentiation of hABCs should be considered when regenerating the alveolar bone using rhBMP-2.-
dc.description.statementOfResponsibilityopen-
dc.relation.isPartOfJOURNAL OF PERIODONTAL RESEARCH-
dc.rightsCC BY-NC-ND 2.0 KR-
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/2.0/kr/-
dc.titleDose- and time-dependent effects of recombinant human bone morphogenetic protein-2 on the osteogenic and adipogenic potentials of alveolar bone-derived stromal cells-
dc.typeArticle-
dc.contributor.collegeCollege of Dentistry (치과대학)-
dc.contributor.departmentDept. of Periodontology (치주과학)-
dc.contributor.googleauthorJ-C. Park-
dc.contributor.googleauthorJ.C. Kim-
dc.contributor.googleauthorB-K. Kim-
dc.contributor.googleauthorK-S. Cho-
dc.contributor.googleauthorG-I. Im-
dc.contributor.googleauthorB-S. Kim-
dc.contributor.googleauthorC-S. Kim-
dc.identifier.doi10.1111/j.1600-0765.2012.01477.x-
dc.admin.authorfalse-
dc.admin.mappingfalse-
dc.contributor.localIdA01041-
dc.contributor.localIdA01653-
dc.contributor.localIdA03810-
dc.relation.journalcodeJ01696-
dc.identifier.eissn1600-0765-
dc.identifier.pmidadipogenesis ; osteogenesis ; recombinant human bone morphogeneticprotein-2 ; stem cells ; tissue engineering-
dc.identifier.urlhttp://onlinelibrary.wiley.com/doi/10.1111/j.1600-0765.2012.01477.x/abstract-
dc.subject.keywordadipogenesis-
dc.subject.keywordosteogenesis-
dc.subject.keywordrecombinant human bone morphogeneticprotein-2-
dc.subject.keywordstem cells-
dc.subject.keywordtissue engineering-
dc.contributor.alternativeNameKim, Chang Sung-
dc.contributor.alternativeNamePark, Jung Chul-
dc.contributor.alternativeNameCho, Kyoo Sung-
dc.contributor.affiliatedAuthorKim, Chang Sung-
dc.contributor.affiliatedAuthorPark, Jung Chul-
dc.contributor.affiliatedAuthorCho, Kyoo Sung-
dc.citation.volume47-
dc.citation.number5-
dc.citation.startPage645-
dc.citation.endPage654-
dc.identifier.bibliographicCitationJOURNAL OF PERIODONTAL RESEARCH, Vol.47(5) : 645-654, 2012-
Appears in Collections:
2. College of Dentistry (치과대학) > Dept. of Periodontics (치주과학교실) > 1. Journal Papers

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