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Chromosomal cephalosporinase in Enterobacter hormaechei as an ancestor of ACT-1 plasmid-mediated AmpC β-lactamase

Authors
 Kyoung Ho Roh  ;  Wonkeun Song  ;  Hae-Sun Chung  ;  Yang Soon Lee  ;  Jong Hwa Yum  ;  Ha Na Yi  ;  Jong Sik Chun  ;  Dongeun Yong  ;  Kyungwon Lee  ;  Yunsop Chong 
Citation
 JOURNAL OF MEDICAL MICROBIOLOGY, Vol.61(pt 1) : 94-100, 2012 
Journal Title
 JOURNAL OF MEDICAL MICROBIOLOGY 
ISSN
 0022-2615 
Issue Date
2012
MeSH
Amino Acid Sequence ; Anti-Bacterial Agents/pharmacology ; Bacterial Proteins/genetics* ; Cefoxitin/pharmacology ; Cephalosporinase/genetics* ; Chromosomes, Bacterial/genetics* ; Enterobacter/classification ; Enterobacter/drug effects ; Enterobacter/genetics* ; Enterobacteriaceae Infections/microbiology ; Humans ; Microbial Sensitivity Tests ; Molecular Sequence Data ; Phylogeny* ; Plasmids/genetics* ; RNA, Ribosomal, 16S/genetics ; Sequence Analysis, DNA ; Species Specificity ; beta-Lactamases/genetics*
Keywords
Amino Acid Sequence ; Anti-Bacterial Agents/pharmacology ; Bacterial Proteins/genetics* ; Cefoxitin/pharmacology ; Cephalosporinase/genetics* ; Chromosomes, Bacterial/genetics* ; Enterobacter/classification ; Enterobacter/drug effects ; Enterobacter/genetics* ; Enterobacteriaceae Infections/microbiology ; Humans ; Microbial Sensitivity Tests ; Molecular Sequence Data ; Phylogeny* ; Plasmids/genetics* ; RNA, Ribosomal, 16S/genetics ; Sequence Analysis, DNA ; Species Specificity ; beta-Lactamases/genetics*
Abstract
In this study of the diversity of AmpC β-lactamase in clinical isolates of Enterobacter spp., a strain was found carrying the plasmid-mediated AmpC β-lactamase ACT-1 gene on its chromosome. The strain was identified as Enterobacter hormaechei using phylogenetic analysis of 16S rRNA and hsp60 genes. In addition, the species was confirmed by DNA-DNA hybridization. The genetic environment of the bla(ACT-1) gene was characterized, including the ampR and ampG genes, using a two-step PCR. The amino acid sequences of AmpR at serine 35, arginine 86, glycine 102, aspartic acid 135 and tyrosine 264 were conserved. Measurement of the transcription level of the bla(ACT-1) gene using real-time quantitative PCR showed that it increased 1.98-fold following cefoxitin induction. These results suggest that the plasmid-mediated bla(ACT-1) gene originated from the chromosome of E. hormaechei.
Files in This Item:
T201200491.pdf Download
DOI
21873382
Appears in Collections:
1. College of Medicine (의과대학) > Dept. of Laboratory Medicine (진단검사의학교실) > 1. Journal Papers
Yonsei Authors
Yong, Dong Eun(용동은) ORCID logo https://orcid.org/0000-0002-1225-8477
Lee, Kyungwon(이경원) ORCID logo https://orcid.org/0000-0003-3788-2134
Lee, Yang Soon(이양순)
Chong, Yun Sop(정윤섭)
Chung, Hae Sun(정혜선)
URI
https://ir.ymlib.yonsei.ac.kr/handle/22282913/90963
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